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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS03280 [old locus tag: SACOL0635 ]
  • pan locus tag?: SAUPAN002369000
  • symbol: SACOL_RS03280
  • pan gene symbol?: lipL
  • synonym:
  • product: biotin/lipoate A/B protein ligase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS03280 [old locus tag: SACOL0635 ]
  • symbol: SACOL_RS03280
  • product: biotin/lipoate A/B protein ligase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 666808..667572
  • length: 765
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (666808..667572) NCBI
  • BioCyc: SACOL_RS03280 BioCyc
  • MicrobesOnline: see SACOL0635

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGCAATCTTTCGCATTCGATGATACATTTTGCGAAAGTGTGGGTAAAGATATATCAGAT
    AATGTTGTGCGTACTTGGATTCATCAACATACTGTTATTCTTGGTATTCATGATTCAAGA
    TTGCCGTTTTTAAAAGATGGCATTGATTATTTAACGAATGAGATTGGTTATAATGCCATT
    GTTAGAAATTCTGGTGGCTTAGGTGTCGTTCTAGATCAAGGTGTATTAAATATATCGCTG
    ATGTTCAAAGGACAAACAGAAACAACGATTGATGAAGCGTTTACTGTGATGTACCTCTTA
    ATTAGCAAAATGTTCGAAAATGAGAATGTTGATATTGATACGATGGAAATTGAACATTCT
    TATTGCCCAGGAAAATTTGACTTAAGTATCGATGGTAAGAAATTTGCAGGCATATCGCAA
    CGAAGAGTTAGAGGCGGTATTGCTGTACAAATTTATCTTTGTGTTGAAGGCTCTGGTTCA
    GAACGTGCATTGATGATGCAAACATTTTATGAACATGCTTTAAAAGGTGAAGTGACTAAA
    TTTAAATATCCTGAAATTGAACCATCTTGTATGGCCTCATTAGAGACATTGCTTAACAAA
    ACGATTACTGTTCAAGATGTAATGTTTTTACTATTATATGCAATCAAAGATCTTGGCGGT
    GTATTAAATATGACGCCAATTACTCAAGAAGAATGGCAAAGATACGATACGTATTTTGAT
    AAAATGATTGAAAGAAACAAGAAAATGATAGATCAAATGCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    765

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS03280 [old locus tag: SACOL0635 ]
  • symbol: SACOL_RS03280
  • description: biotin/lipoate A/B protein ligase family protein
  • length: 254
  • theoretical pI: 4.6258
  • theoretical MW: 28953.1
  • GRAVY: -0.118898

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.204?  ExPASy
    Octanoyl-[GcvH]:protein N-octanoyltransferase [Glycine cleavage system H]-N6-octanoyl-L-lysine + a [lipoyl-carrier protein] = glycine cleavage system H + a [lipoyl-carrier protein]-N6-octanoyl-L-lysine
  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair lipoyltransferase and lipoate-protein ligase (TIGR00545; HMM-score: 14.8)
  • TheSEED: see SACOL0635
  • PFAM:
    tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 45.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00439
    • TAT(Tat/SPI): 0.00014
    • LIPO(Sec/SPII): 0.000678
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQSFAFDDTFCESVGKDISDNVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNISLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFAGISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASLETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQMQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: see SACOL0635
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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