⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA2159 [new locus tag: SA_RS12395 ]
pan locus tag^?: SAUPAN005885000
symbol: SA2159
pan gene symbol^?: paiA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2159 [new locus tag: SA_RS12395 ]
• symbol: SA2159
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2426607..2427122
• length: 516
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125087 NCBI
• RefSeq: NP_375482 NCBI
• BioCyc: see SA_RS12395
• MicrobesOnline: 104508 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
  
  ATGGCTGGGATAATCAAAGAAATTTCAGAACAAAATGCTAGTGAATTAGTTGAATTAGCA
  ACTAGAACATTTTATGACACGTTTGGTTCTTACTATGATGACAAAGATTTTGATCAATTT
  TTTAAAGACAATTATACTGTAGAAAAATTTACACAAGAGATTAACCATGTAGATTCATTT
  CATTATTTTTATCAAGAAGATGGTGCGAATGTTGGTTATATAAAGATGAATATTAATAGT
  GCTCAAACTGAAGAAATGGGGGAGACCTATTTAGAAGTGCAGCGCATATATTTTTTGAAA
  GACTTTCAAGGTGGCGGAAGAGGTTCACAATTGATAGAATTGGCCGAAAAAATTGCTCAA
  GAACATAATAAACATAAAATTTGGCTAGGAGTTTGGGAGCATAATCCTCGTGCTCAAGCA
  TTTTATAAACGTCATGGATTTAAAGTGGTTGGTGAACATCATTTTCAAACAGGTGATGTA
  ACGGATACAGATTTAATTATGGAAAAAGAGTTATAA
  60
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  516
  

⊟Protein

⊟General

• locus tag: SA2159 [new locus tag: SA_RS12395 ]
• symbol: SA2159
• description: hypothetical protein
• length: 171
• theoretical pI: 4.71992
• theoretical MW: 20071.1
• GRAVY: -0.690643

⊟Function

• ⊞⊞TIGRFAM:
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal-protein-alanine acetyltransferase (TIGR01575; EC 2.3.1.128; HMM-score: 49.4)
  and 2 more
  UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase (TIGR03585; EC 2.3.1.202; HMM-score: 27.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs mycothiol synthase (TIGR03448; EC 2.3.1.189; HMM-score: 18.1)
• TheSEED  :
  • N1-spermidine/spermine acetyltransferase PaiA
• ⊞⊞PFAM:
  Acetyltrans (CL0257) Acetyltransf_1; Acetyltransferase (GNAT) family (PF00583; HMM-score: 67.2)
  and 8 more
  Acetyltransf_7; Acetyltransferase (GNAT) domain (PF13508; HMM-score: 51.4)
  Acetyltransf_10; Acetyltransferase (GNAT) domain (PF13673; HMM-score: 49.7)
  Acetyltransf_4; Acetyltransferase (GNAT) domain (PF13420; HMM-score: 28.4)
  Acetyltransf_3; Acetyltransferase (GNAT) domain (PF13302; HMM-score: 25.5)
  FR47; FR47-like protein (PF08445; HMM-score: 24.3)
  FeeM; N-acyl amino acid synthase FeeM (PF21926; HMM-score: 18.6)
  no clan defined NKWYS; Putative capsular polysaccharide synthesis protein (PF10364; HMM-score: 16.1)
  Acetyltrans (CL0257) Acetyltransf_18; Acetyltransferase (GNAT) domain (PF18014; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9998
  • Cytoplasmic Membrane Score: 0
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0002
• ⊞⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003821
  • TAT(Tat/SPI): 0.000299
  • LIPO(Sec/SPII): 0.001179
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927949 NCBI
• RefSeq: NP_375482 NCBI
• UniProt: A0A0H3JNA1 UniProt

⊟Protein sequence

• MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDNYTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDFQGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDVTDTDLIMEKEL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications