PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_2274 [new locus tag: NWMN_RS13085 ]
pan locus tag^?: SAUPAN005890000
symbol: NWMN_2274
pan gene symbol^?: iruO
• synonym:
• product: pyridine nucleotide-disulfide oxidoreductase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2274 [new locus tag: NWMN_RS13085 ]
• symbol: NWMN_2274
• product: pyridine nucleotide-disulfide oxidoreductase family protein
• replicon: chromosome
• strand: -
• coordinates: 2497724..2498758
• length: 1035
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331388 NCBI
• RefSeq: YP_001333308 NCBI
• BioCyc:
• MicrobesOnline: 3707876 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  481
  541
  601
  661
  721
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  841
  901
  961
  1021
  
  ATGAAAGATGTTACAATCATTGGTGGCGGTCCGTCTGGTTTGTATGCAAGCTTTTATGCT
  GGATTGCGAGATATGTCTGTAAGATTGATAGATGTTCAATCTGAATTAGGGGGTAAGATG
  AGAATTTACCCTGAAAAAATTATTTGGGATATTGGCGGCATTGCGCCAAAACCTTGTCAT
  GAAATTTTAAAAGATACAATTAAGCAAGGATTATATTTTAAACCGGAAGTTCATTTGAAT
  GAACGTGTGGTAGATATTAGGAAAAAGGCAGAACGTCATTTCGAAGTTGAAACAGAAGCG
  GGAGAAATATATACATCGAAAGCAGTTATCATTGCAATTGGTGCGGGTATTATTAATCCA
  AAACAATTAGATGTTAAAGGTGTAGAGAGATATCAATTAACTAATTTACATTATGTTGTA
  CAAAGTTACAGACGTTTCAAAGATAAAGATGTATTAATATCAGGTGGAGGAAATACAGCA
  TTAGATTGGGCGCATGATATTGCCAAGATTGCTAAAAGCGTGACAGTTGTTTATCGAAAA
  GAAGATGTAAGTGGTCATGAAGCGATGAAAACGTTGGTGACAGATTTAAATGTGAAACTA
  TGCCCAAAAACACGTATTAAATACTTGGTCGGCAATGATGATGAAACGCATATAAGTGAA
  GTTGTTTTGGAACATGTTGAAAGTGGCGATAGGCATACAGTTAAATTTGACGATGTCATT
  ATTAGTCATGGGTTTGATCGTTGTAATACATTATTGAGTGAAACATCTTCAAAGCTAGAT
  ATGCATGATGATTGTCGTGTTAAAGGATTTGGTAATACGACAACTAGTATACCTGGTATC
  TATGCGTGTGGAGATATTGTTTATCATGATGCAAAATCACATCTAATTGCAAGTGCATTT
  AGTGATGGTGCAAATGCAGCGAACCTTGCTAAAACATATATCCAACCAGATGCGAATGCA
  GAGGGTTATGTTTCAAGTCATCATGAAGTGTTTAAAGAAGCGAATAAGACTATTGTAAAT
  AAACATTTATACTAG
  60
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  360
  420
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  1020
  1035
  

⊟Protein

• 5TWB
  PDB
• 5TWC
  PDB

Protein Data Bank: 5TWC

⊟General

• locus tag: NWMN_2274 [new locus tag: NWMN_RS13085 ]
• symbol: NWMN_2274
• description: pyridine nucleotide-disulfide oxidoreductase family protein
• length: 344
• theoretical pI: 6.95916
• theoretical MW: 38229.3
• GRAVY: -0.27936

⊟Function

• ⊞reaction:
  EC 1.18.1.2?  ExPASy
  Ferredoxin--NADP⁺ reductase 2 reduced ferredoxin + NADP⁺ + H⁺ = 2 oxidized ferredoxin + NADPH
• ⊞TIGRFAM:
  Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 130)
  and 27 more
  Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 92.5)
  Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 92.5)
  putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 86.7)
  Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 76.3)
  dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 55.4)
  Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 46.4)
  Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 40.8)
  putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 40.8)
  Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 40.2)
  mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 36.3)
  Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 35)
  Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 34.5)
  glutamate synthase, small subunit (TIGR01318; HMM-score: 32.4)
  mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 30.9)
  thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 30.7)
  glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 28)
  Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 27.7)
  mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 27.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 24.5)
  Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 23.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 23)
  squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 19.5)
  lycopene cyclase family protein (TIGR01790; HMM-score: 18.2)
  flavin-dependent oxidoreductase, MSMEG_0569 family (TIGR04046; HMM-score: 17.7)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 14.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin protoporphyrinogen oxidase (TIGR00562; EC 1.3.3.4; HMM-score: 13.1)
  FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 11.5)
• ⊞TheSEED  :
  • Thioredoxin reductase (EC 1.8.1.9)
  Nucleosides and Nucleotides Pyrimidines pyrimidine conversions  Thioredoxin reductase (EC 1.8.1.9)
  and 1 more
  Sulfur Metabolism Sulfur Metabolism - no subcategory Thioredoxin-disulfide reductase  Thioredoxin reductase (EC 1.8.1.9)
• ⊞PFAM:
  NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 110.3)
  and 14 more
  Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 83.2)
  Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 46.1)
  HI0933_like; HI0933-like protein Rossmann domain (PF03486; HMM-score: 43.8)
  NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 30.6)
  Lys_Orn_oxgnase; L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase (PF13434; HMM-score: 28.9)
  DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 26.6)
  Thi4; Thi4 family (PF01946; HMM-score: 24.2)
  FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 23.1)
  NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 21.7)
  GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 21.4)
  FAD_binding_2; FAD binding domain (PF00890; HMM-score: 19.9)
  Amino_oxidase; Flavin containing amine oxidoreductase (PF01593; HMM-score: 19.9)
  NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 15.3)
  FMO-like; Flavin-binding monooxygenase-like (PF00743; HMM-score: 15.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: FAD
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9009
  • Cytoplasmic Membrane Score: 0.0839
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0151
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005715
  • TAT(Tat/SPI): 0.000504
  • LIPO(Sec/SPII): 0.001405
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222486 NCBI
• RefSeq: YP_001333308 NCBI
• UniProt: A6QJL4 UniProt

⊟Protein sequence

• MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYPEKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGEIYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTALDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHISEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTSIPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEANKTIVNKHLY

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2273 < NWMN_2274

⊟Regulation

• ⊞regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Slade A Loutet, Marek J Kobylarz, Crystal H T Chau, Michael E P Murphy
IruO is a reductase for heme degradation by IsdI and IsdG proteins in Staphylococcus aureus.
J Biol Chem: 2013, 288(36);25749-59
[PubMed:23893407] [WorldCat.org] [DOI] (I p)