From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1013 [new locus tag: SACOL_RS05175 ]
  • pan locus tag?: SAUPAN003180000
  • symbol: mgtE
  • pan gene symbol?: mgtE
  • synonym:
  • product: magnesium transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1013 [new locus tag: SACOL_RS05175 ]
  • symbol: mgtE
  • product: magnesium transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 1020124..1021509
  • length: 1386
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCAATGAACACAGATGAAAAAGAGCGTGTTCAAGAGGAATTATACGACCAAACTTTA
    TTAGATCAATATTTAGAAAATGATGATATTGATCAATTTAGAGATGAATTTCTAGCATTA
    CACACATATGAACAAAGTGAGTATTTTGAAGATACTACCGATGAAAATAGACAAAAGATT
    TTTCAATATTTATCACCTGAAGAAGTTGCAAATTTCTTTGATCAATTAGATATTGATGAC
    GATGAATATGAGTTGCTATTTGATAAGATGAATGCGACATACGCAAGTCACATATTAGAA
    GAAATGTCATACGACAATGCAGTAGATATTTTAAATGAGTTGACTAAACCAAAAGTTGCT
    AGTCTTTTAACATTGATGAATAAAGATGACGCGAATGAAATCAAAGCATTACTTCACTAT
    GATGAGGATACGGCCGGCGGTATTATGACGACGGAGTATTTATCACTTAAAGCGCATACG
    CCTGTTAAAGAAGCATTATTATTGGTCAAAGCGCAAGCACCAGACGCAGAAACAATATAT
    GTTATATTTGTCGTTGATGATGATGGTAAATTAGTAGGTGTTTTATCGCTAAGAGATTTA
    ATTGTAGCTGAAAATGATGCTTATATTGAAGATATTATGAATGAACGTGTCATTAGTGTG
    AATGTAGCAGACGACCAAGAAGATGTTGCTCAAGTTATGAGAGACTATGATTTCATGGCT
    GTACCTGTTATAGATTACCAAGAACATTTGCTTGGTATCATCACGATTGATGATATTTTA
    GACGTTATGGATGAAGAGGCTAGTGAAGACTACTCTCGTTTAGCCGGGGTATCAGATATC
    GATTCGACTAATGATTCAATCATTAAAACAGCATTAAAACGTTTACCATGGTTGATTATT
    TTAACATTTTTAGGAATGATTACTGCGACAATTTTAGGGAGATTCGAAAAAACATTAGAA
    AATGTAGCGCTACTCGCAGCGTTTATTCCTATTATTAGTGGTATGTCAGGAAATTCAGGT
    ACACAATCTTTAGCCGTTTCAGTTCGTAACATTACGACAGGGGAAATTAATGAGCAAAGT
    AAATTTAGAATTGCATTAAGAGAAGCAGGAAGTGGTGTATTATCGGGTGTTGTATGTTCA
    ACAATATTATTTACAATTATTGTTGCAATATATCATCAGCCACTTTTAGCATTAATCGTT
    GCAGGAAGTTTAACTTGTGCGATGACGGTGGGGACGTTTGTAGGTTCGATGATTCCATTA
    TTGATGAATAAATTAAATATCGATCCAGCAGTGGCTAGTGGACCATTTATTACAACAATT
    AATGATATTATTAGTATGTTGATTTATTTTGGTTTAGCTACATCATTTATGGCTTACTTA
    ATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1386

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1013 [new locus tag: SACOL_RS05175 ]
  • symbol: MgtE
  • description: magnesium transporter
  • length: 461
  • theoretical pI: 3.87276
  • theoretical MW: 51435.2
  • GRAVY: 0.13731

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds magnesium transporter (TIGR00400; HMM-score: 405.1)
    and 6 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 39.8)
    Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 32.2)
    Unknown function General IMP dehydrogenase family protein (TIGR01303; HMM-score: 18.5)
    glutaredoxin-like protein, YruB-family (TIGR02196; HMM-score: 15.6)
    Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 13.5)
    Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 12.7)
  • TheSEED  :
    • CBS domain
    • Mg/Co/Ni transporter MgtE
  • ⊞PFAM:
    no clan defined MgtE; Divalent cation transporter (PF01769; HMM-score: 120.1)
    and 5 more
    FliG (CL0436) MgtE_N; MgtE intracellular N domain (PF03448; HMM-score: 81)
    no clan defined CBS; CBS domain (PF00571; HMM-score: 79.3)
    GAF (CL0161) GAF; GAF domain (PF01590; HMM-score: 13.4)
    Acetyltrans (CL0257) Acetyltransf_1; Acetyltransferase (GNAT) family (PF00583; HMM-score: 12.2)
    no clan defined TcpE; TcpE family (PF12648; HMM-score: 9.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9993
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0007
  • ⊞LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002358
    • TAT(Tat/SPI): 0.00418
    • LIPO(Sec/SPII): 0.000491
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYEQSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEMSYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHTPVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVISVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAGVSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISGMSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYHQPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYFGLATSFMAYLI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL1013&oldid=71671"