PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA0932 [new locus tag: SA_RS05285 ]
pan locus tag^?: SAUPAN003301000
symbol: SA0932
pan gene symbol^?: rlmQ
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0932 [new locus tag: SA_RS05285 ]
• symbol: SA0932
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1058737..1059909
• length: 1173
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123756 NCBI
• RefSeq: NP_374200 NCBI
• BioCyc: see SA_RS05285
• MicrobesOnline: 103226 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAAAATAGCAACTCTGAACAAAGGCAAAGAAACAAAATATTTTAATGGATATCCTTTA
  ATTGAAGAAGAGGATATCTATTCACAAGATCATTTAAAAGAAGGAGATATTTTTCAAATT
  GTGACTGATAAATCACAATATGTTGCAACGGCTTATGTTGGTCGCCAACATAAAGGATTA
  GGTTGGGTTCTAACATACGATAAAGCTCAAGAAATCAACACAGCTTTCTTTGTGAAATTG
  TTTAATACTGCATTAGCAGAACGTGATTATTATTTTAATATAGATGGAACAAATGCTTTT
  AGATTATTTAATGCTGAAGGTGATGGTGTTGGGGGATTAACAATCGACAATTACGATGGT
  CATTTGTTGATTCAATGGTACTCAAAAGGTATTTATAAATTTAAATATGCCATTCTTGAA
  GCGGTTAGAAAAGTATTTGATTATAAATCTATTTACGAAAAAGTAAGATTTAAAGACAGC
  GAATATAGTGGTGGTTTTGTTGAAGGCGATGCACCTGAGTTTCCAATTGTTATCGAAGAA
  AACTTCACATTTTATAATGTAGACCTTGAAGATGGTTTGATGACAGGTATCTTTTTAGAT
  CAAAAAGAAGTGCGCAAGAAATTAAGGGGTCAATATGCCAAAGAACGCCATGTTTTAAAC
  TTATTTAGTTATACAGGTGCTTTTTCTGTAATAGCAGCAAGTGAGGCATCTTCAACAACA
  AGTGTAGATTTGGCTAATCGTTCTCGTAGTTTAACTGAAGAAAATTTTGGATTAAATGCT
  ATTGATCCTAAATCCCAATATATTTATGTCATGGACACTTTTGATTTCTATAAATATGCT
  GCACGACATGGACATAGTTATGACACGATCGTGATTGATCCACCTAGCTTTGCACGCAAC
  AAAAAACGTACATTTTCAGTGCAAAAAGATTATGACAAATTAATTAATGGCGCCTTAAAT
  ATTTTATCATCTGAAGGAACATTATTGTTATGTACAAATGCAAGTGTATATCCATTAAAG
  CAATTTAAAAATACTATTAAAAAGACGCTTGAAGAGAGTGGCGTTGATTATGAATTAACT
  GAAGTTATGGGTTTACCAAAAGATTTTAAAACGCATCCACATTATAAGCCATCTAAATAT
  TTAAAAGCTGTTTTTGTAAATATTAGACATTAA
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⊟Protein

⊟General

• locus tag: SA0932 [new locus tag: SA_RS05285 ]
• symbol: SA0932
• description: hypothetical protein
• length: 390
• theoretical pI: 6.80756
• theoretical MW: 44738.2
• GRAVY: -0.411026

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD (TIGR00095; EC 2.1.1.171; HMM-score: 21.5)
• TheSEED  :
  • Putative ribosomal RNA large subunit methyltransferase YwbD
  Protein Metabolism Protein biosynthesis Ribosome biogenesis bacterial  LSU m5C1962 methyltransferase RlmI
• ⊞PFAM:
  NADP_Rossmann (CL0063) Methyltrans_SAM; S-adenosylmethionine-dependent methyltransferase (PF10672; HMM-score: 81.9)
  and 6 more
  PUA (CL0178) PUA_3; PUA-like domain (PF17785; HMM-score: 58.1)
  NADP_Rossmann (CL0063) Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 27.6)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 23.2)
  TRM5-TYW2_MTfase; TRM5/TYW2 methyltransferase domain (PF02475; HMM-score: 16.3)
  RsmJ; Ribosomal RNA large subunit methyltransferase D, RlmJ (PF04378; HMM-score: 11.3)
  N6-adenineMlase; Probable N6-adenine methyltransferase (PF10237; HMM-score: 8.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9638
  • Cytoplasmic Membrane Score: 0.0184
  • Cell wall & surface Score: 0.0026
  • Extracellular Score: 0.0152
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004144
  • TAT(Tat/SPI): 0.000136
  • LIPO(Sec/SPII): 0.000446
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926667 NCBI
• RefSeq: NP_374200 NCBI
• UniProt: A0A0H3JTS6 UniProt

⊟Protein sequence

• MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDKSQYVATAYVGRQHKGLGWVLTYDKAQEINTAFFVKLFNTALAERDYYFNIDGTNAFRLFNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDSEYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRGQYAKERHVLNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDFYKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNASVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0932 > SA0933

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications