PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_1750 [new locus tag: NWMN_RS09860 ]
pan locus tag^?: SAUPAN004794000
symbol: NWMN_1750
pan gene symbol^?: glnP
• synonym:
• product: extracellular glutamine-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1750 [new locus tag: NWMN_RS09860 ]
• symbol: NWMN_1750
• product: extracellular glutamine-binding protein
• replicon: chromosome
• strand: -
• coordinates: 1950909..1952366
• length: 1458
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331038 NCBI
• RefSeq: YP_001332784 NCBI
• BioCyc:
• MicrobesOnline: 3707310 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAGTGTTTAATTAGGTTTATATTAGTATTAGGGTTGTTAATTAGCAGTGCTATGGTG
  TATATCAATCCCACAGCACATGCTGAACAAGATCAAACATGGGAGAAGATTAAAGAACGC
  GGTGAACTTAGAGTGGGTCTTTCTGCAGATTATGCACCAATGGAATTTGAGCATACAGTT
  AATGGTAAGACTGAGTATGCAGGTGTAGATATTGATTTAGCTAAAAAAATTGCGAAAGAT
  AATAATTTAAAATTAAAAATCGTCAATATGTCATTTGATAGTTTGTTAGGAGCTCTTAAA
  ACTGGAAAAATTGATATTATTATTTCCGGAATGACTTCAACGCCTGAACGTAAGAAGCAA
  GTTGATTTTTCAGATTCATATATGATGACTAAAAATATCATGCTTGTAAAGAAAGATAAA
  GTTAATGAATATAAAGATATCAAAGACTTTAATAATAAAAAAGTAGGGGCACAAAAGGGA
  ACTGAACAAGAAAAAATCGCTCAAACCGAAATTGAAAATGCATCTATTACTTCATTAAGC
  CGTTTACCAGACGTTATATTAGCATTGAAAAGTGGAAAGGTTGAAGGTGCTGTAGTTGAA
  AAACCTGTAGCAGAAGCATATTTAAAACAAAATCCTAAATTAGGAATTTCCAATGTGAAA
  TTTAATGAAGAAGAAAAAGATACAGTGATAGCAGTGCCAAAAGATTCACCAAAATTATTG
  TCACAAATTAATAAAACGATTAAGGAGGTTAAAGATAAAGGATTAATCGATAAATATATG
  ACTAATGCTGCAAATGCGATGAATGATGACAGTGGTTTTATTTCTAAGTATGGTAGTTTT
  TTCTTGAAAGGAATTAAGATTACAATATTAATTTCACTTATCGGTGTTGCATTAGGTTCT
  ATTTTAGGTGCATTCGTTGCGTTAATGAAATTAAGTAAAATAAAAATTATTTCATGGATT
  GCTTCTATCTATATCGAAATATTAAGAGGAACACCAATGTTAGTTCAAGTATTTATCGTA
  TTCTTTGGTATAACTGCTGCATTAGGACTAGACATTTCAGCTCTTGTTTGTGGAACAATT
  GCATTAGTTATTAATTCCTCAGCTTACATTGCTGAAATTATTCGTGCAGGTATAAATGCT
  GTTGATAAAGGCCAAATGGAAGCCGCACGTAGTCTAGGTTTGAATTATAGACAAACGATG
  AAAAGTGTAATTATGCCACAAGCAATTAAAAATATTTTACCAGCTTTAGGTAATGAATTT
  GTCACTTTAATTAAAGAATCATCTATTGTGTCAACAATTGGAGTTGGCGAAATTATGTTT
  AATGCACAAGTGGTTCAAGGTATTTCATTTGACCCATTCACACCATTAATAGTGGCAGCA
  GCATTATACTTTGTCTTAACATTTGTACTTACACGCATTATGAACATGATTGAAGGGAGA
  TTGAATGCCAGTGATTAA
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⊟Protein

⊟General

• locus tag: NWMN_1750 [new locus tag: NWMN_RS09860 ]
• symbol: NWMN_1750
• description: extracellular glutamine-binding protein
• length: 485
• theoretical pI: 9.56041
• theoretical MW: 53122.2
• GRAVY: 0.212371

⊟Function

• ⊞TIGRFAM:
  ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 159)
  ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 153.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines lysine-arginine-ornithine-binding periplasmic protein (TIGR01096; HMM-score: 136.6)
  and 12 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 98.5)
  Metabolism Transport and binding proteins Amino acids, peptides and amines ectoine/hydroxyectoine ABC transporter solute-binding protein EhuB (TIGR02995; HMM-score: 59.7)
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 45.9)
  extracellular substrate-binding orphan protein, GRRM family (TIGR04262; HMM-score: 41.1)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 29.8)
  Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 22.6)
  Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 22.6)
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 18.7)
  NifC-like ABC-type porter (TIGR01581; HMM-score: 18.2)
  Hypothetical proteins Conserved conserved hypothetical protein (TIGR02285; HMM-score: 14.5)
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 11.9)
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 11.6)
• TheSEED  :
  • Arginine ABC transporter, substrate-binding and permease protein
• ⊞PFAM:
  PBP (CL0177) SBP_bac_3; Bacterial extracellular solute-binding proteins, family 3 (PF00497; HMM-score: 123)
  and 4 more
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 88)
  PBP (CL0177) Lig_chan-Glu_bd; Ligated ion channel L-glutamate- and glycine-binding site (PF10613; HMM-score: 35.3)
  NMT1; NMT1/THI5 like (PF09084; HMM-score: 14.6)
  no clan defined DUF2085; Predicted membrane protein (DUF2085) (PF09858; HMM-score: 9.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 4
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9999
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0001
• ⊞LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: Signal peptide SP(Sec/SPI) length 28 aa
  • SP(Sec/SPI): 0.995819
  • TAT(Tat/SPI): 0.001914
  • LIPO(Sec/SPII): 0.001537
  • Cleavage Site: CS pos: 28-29. AHA-EQ. Pr: 0.9908
• predicted transmembrane helices (TMHMM): 3

⊟Accession numbers

• GI: 151221962 NCBI
• RefSeq: YP_001332784 NCBI
• UniProt: A0A0H3K9A2 UniProt

⊟Protein sequence

• MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELRVGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTGKIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKGTEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISNVKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISKYGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPMLVQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSLGLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISFDPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1749 < NWMN_1750

⊟Regulation

• ⊞regulators: ArgR* (repression) regulon, CodY (repression) regulon

  ArgR*	(TF)	important in Arginine biosynthesis, Arginine degradation; RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications