⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS04770 [old locus tag: SACOL0930 ]
pan locus tag^?: SAUPAN003026000
symbol: SACOL_RS04770
pan gene symbol^?: —
• synonym:
• product: DUF86 domain-containing protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS04770 [old locus tag: SACOL0930 ]
• symbol: SACOL_RS04770
• product: DUF86 domain-containing protein
• replicon: chromosome
• strand: +
• coordinates: 935101..935535
• length: 435
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (935101..935535) NCBI
• BioCyc: SACOL_RS04770 BioCyc
• MicrobesOnline: see SACOL0930

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTATTTTGTAGACAAAGATAAACTAACTCAGAAATTAGCCTATTTACAAGCATTAACT
  GATGATTATCATGAGAGCAAGCACAATCATTATGCATTTGAACGCATTGCTCAAATGTTG
  ATAGAATCATCGGTAGATATAGGGAATATGATTATCGATGCATTTATTTTAAGGGATCCT
  GGTAATTATAAAGATGTGATTGATATATTAGAACTAGAAAATGTTATTACTAAAGAAACA
  CAGCAGGCGATTAATAAAACTGTCGGTATTCGTAAACAATTTACATATGATTACACAGCC
  TTAGATATTAAGATTATCATGCCAATGTTTGATGACGCATTACCTTATTACAAACAATTT
  ATTACAGAAGTAACGACATTTTTACATCAAGAAAATGTACCAGTAACAGCTTTTGGTAAA
  GGAGAAAATCAATAA
  60
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  435
  

⊟Protein

⊟General

• locus tag: SACOL_RS04770 [old locus tag: SACOL0930 ]
• symbol: SACOL_RS04770
• description: DUF86 domain-containing protein
• length: 144
• theoretical pI: 4.64579
• theoretical MW: 16779
• GRAVY: -0.2875

⊟Function

• TIGRFAM:
• TheSEED: see SACOL0930
• PFAM:
  KNTase_C (CL0291) HepT-like; Ribonuclease HepT-like (PF01934; HMM-score: 75.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9894
  • Cytoplasmic Membrane Score: 0.008
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0023
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001402
  • TAT(Tat/SPI): 0.000084
  • LIPO(Sec/SPII): 0.000266
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446196126 NCBI
• RefSeq: WP_000273981 NCBI
• UniProt: see SACOL0930

⊟Protein sequence

• MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESSVDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALDIKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ

⊟Experimental data

• experimentally validated: see SACOL0930
• protein localization: see SACOL0930
• quantitative data / protein copy number per cell: see SACOL0930
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL0930

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications