⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS01115 [old locus tag: SACOL0218 ]
pan locus tag^?: SAUPAN001103000
symbol: SACOL_RS01115
pan gene symbol^?: —
• synonym:
• product: DUF488 domain-containing protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS01115 [old locus tag: SACOL0218 ]
• symbol: SACOL_RS01115
• product: DUF488 domain-containing protein
• replicon: chromosome
• strand: -
• coordinates: 259820..260176
• length: 357
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (259820..260176) NCBI
• BioCyc: SACOL_RS01115 BioCyc
• MicrobesOnline: see SACOL0218

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  
  ATGACCGTAGATATTGGACGGATTTATGACAATAAAGATAATACCGACGCTATTCGTATC
  CTAGTCGATAGAGTCTGGCCGAGAGGTATTTCGAAAAGAACTGCTAACCTAGATTATTGG
  TTAAAAGACATTGCCCCTTCTACTGAGTTGCGACAATGGTTCCAACATGATCCTAAACTT
  TTTGGAGCTTTTAAAGAAAAATATGAAAAAGAATTACGTGATCAGGATGCGCAAAAAGAT
  GCTTTTGAAAAATTAAAGGATATTGTAAATCAGCATAATCATGTTCTATTGTTATATGCA
  GCAAAAGATACTAAACATAACCAAGCTGTAGTACTACAGCAGTTGCTCAATACTTAG
  60
  120
  180
  240
  300
  357
  

⊟Protein

⊟General

• locus tag: SACOL_RS01115 [old locus tag: SACOL0218 ]
• symbol: SACOL_RS01115
• description: DUF488 domain-containing protein
• length: 118
• theoretical pI: 8.87228
• theoretical MW: 13934.7
• GRAVY: -0.734746

⊟Function

• TIGRFAM:
• TheSEED: see SACOL0218
• ⊞⊞PFAM:
  Phosphatase (CL0031) DUF488-N3i; Inactive DUF488-N3 subclade (PF22752; HMM-score: 120.4)
  DUF488; Domain of unknown function DUF488 (PF04343; HMM-score: 112.5)
  and 2 more
  DUF488-N3a; Active DUF488-N3 subclade (PF22751; HMM-score: 85.8)
  no clan defined Inhibitor_I29; Cathepsin propeptide inhibitor domain (I29) (PF08246; HMM-score: 14.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.995
  • Cytoplasmic Membrane Score: 0.0003
  • Cell wall & surface Score: 0.0003
  • Extracellular Score: 0.0044
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002823
  • TAT(Tat/SPI): 0.000174
  • LIPO(Sec/SPII): 0.000548
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446137361 NCBI
• RefSeq: WP_000215216 NCBI
• UniProt: see SACOL0218

⊟Protein sequence

• MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDIAPSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAKDTKHNQAVVLQQLLNT

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications