PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS00555 [old locus tag: SACOL0111 ]
pan locus tag^?: SAUPAN000932000
symbol: SACOL_RS00555
pan gene symbol^?: butA
• synonym:
• product: diacetyl reductase ((S)-acetoin forming)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS00555 [old locus tag: SACOL0111 ]
• symbol: SACOL_RS00555
• product: diacetyl reductase ((S)-acetoin forming)
• replicon: chromosome
• strand: +
• coordinates: 126369..127145
• length: 777
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (126369..127145) NCBI
• BioCyc: SACOL_RS00555 BioCyc
• MicrobesOnline: see SACOL0111

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACAAACAACAAAGTAGCATTAGTAACTGGCGGAGCACAAGGGATTGGTTTTAAAATT
  GCAGAACGTTTAGTGGAAGATGGTTTCAAAGTAGCAGTTGTTGATTTCAATGAAGAAGGG
  GCAAAAGCAGCTGCACTTAAATTATCAAGTGATGGTACAAAAGCTATTGCTATCAAAGCA
  GATGTATCAAACCGTGATGATGTATTTAACGCAGTAAGACAAACTGCCGCGCAATTTGGC
  GATTTCCATGTCATGGTTAACAATGCCGGCCTTGGACCAACAACACCAATCGATACAATT
  ACTGAAGAACAGTTTAAAACAGTATATGGCGTGAACGTTGCAGGTGTGCTATGGGGTATT
  CAAGCCGCACATGAACAATTTAAAAAATTCAATCATGGCGGTAAAATTATCAATGCAACA
  TCTCAAGCAGGCGTTGAGGGTAACCCAGGCTTGTCTTTATATTGCAGTACAAAATTCGCA
  GTGCGAGGTTTAACACAAGTAGCCGCACAAGATTTAGCGTCTGAAGGTATTACTGTGAAT
  GCATTCGCACCTGGTATCGTTCAAACACCAATGATGGAAAGTATCGCAGTGGCAACAGCC
  GAAGAAGCAGGTAAACCTGAAGCATGGGGTTGGGAACAATTTACAAGTCAGATTGCTTTG
  GGCAGAGTTTCTCAACCAGAAGATGTTTCAAATGTAGTGAGCTTCTTAGCTGGTAAAGAC
  TCTGATTACATTACTGGACAAACAATTATTGTAGATGGTGGTATGAGATTCCGTTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS00555 [old locus tag: SACOL0111 ]
• symbol: SACOL_RS00555
• description: diacetyl reductase ((S)-acetoin forming)
• length: 258
• theoretical pI: 4.79367
• theoretical MW: 27215.5
• GRAVY: -0.0108527

⊟Function

• ⊞reaction:
  EC 1.1.1.304?  ExPASy
  Diacetyl reductase ((S)-acetoin forming) (S)-acetoin + NAD⁺ = diacetyl + NADH
• ⊞TIGRFAM:
  Metabolism Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 418.5)
  and 19 more
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 190.3)
  3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 184.5)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 170.7)
  acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 155.2)
  2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 153.3)
  2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 151.9)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 132.1)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 128.7)
  rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 128.2)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 127.3)
  cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 101.2)
  pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 92.3)
  sepiapterin reductase (TIGR01500; EC 1.1.1.153; HMM-score: 42.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll light-dependent protochlorophyllide reductase (TIGR01289; EC 1.3.1.33; HMM-score: 18.5)
  polyketide-type polyunsaturated fatty acid synthase PfaA (TIGR02813; HMM-score: 16.8)
  Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 16)
  NAD dependent epimerase/dehydratase, LLPSF_EDH_00030 family (TIGR04180; HMM-score: 14.5)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 14.1)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides ADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 12.1)
• TheSEED: see SACOL0111
• ⊞PFAM:
  NADP_Rossmann (CL0063) adh_short; short chain dehydrogenase (PF00106; HMM-score: 190.9)
  adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 190.1)
  and 10 more
  KR; KR domain (PF08659; HMM-score: 58.9)
  SDR; SDR-like rossmann domain (PF23441; HMM-score: 30)
  Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 17.2)
  Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 15.8)
  3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 15.1)
  Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 14.6)
  no clan defined Dynein_attach_N; Dynein attachment factor N-terminus (PF15867; HMM-score: 14.5)
  NADP_Rossmann (CL0063) TrkA_N; TrkA-N domain (PF02254; HMM-score: 14.1)
  PDDEXK (CL0236) HFX_2341_N; HFX_2341-like, N-terminal (PF19810; HMM-score: 13.4)
  NADP_Rossmann (CL0063) GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8755
  • Cytoplasmic Membrane Score: 0.0516
  • Cell wall & surface Score: 0.0024
  • Extracellular Score: 0.0705
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005476
  • TAT(Tat/SPI): 0.000558
  • LIPO(Sec/SPII): 0.000737
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446105916 NCBI
• RefSeq: WP_000183771 NCBI
• UniProt: see SACOL0111

⊟Protein sequence

• MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAAALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITEEQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFAVRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQIALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR

⊟Experimental data

• experimentally validated: see SACOL0111
• protein localization: see SACOL0111
• quantitative data / protein copy number per cell: see SACOL0111
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SACOL0111

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL0111

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications