PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA0524 [new locus tag: SA_RS03050 ]
pan locus tag^?: SAUPAN002343000
symbol: SA0524
pan gene symbol^?: folE2
• synonym:
• product: GTP cyclohydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0524 [new locus tag: SA_RS03050 ]
• symbol: SA0524
• product: GTP cyclohydrolase
• replicon: chromosome
• strand: -
• coordinates: 619909..620787
• length: 879
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123329 NCBI
• RefSeq: NP_373777 NCBI
• BioCyc: see SA_RS03050
• MicrobesOnline: 102803 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGACTGAATTTGATTTATCGACACGAGAAGGTCGTTGGAAACACTTTGGTTCCGTAGAT
  CCTATTGAAGGCACGAAACCTACTACTAAGAACGAAATGACAGATTTACAAAGTACACAT
  AAAGATTTTTTATTTGAAATAGAAGAAGTTGGTATTAAAAACTTAGTGTACCCTGTACTT
  GTTGACCAGTATCAAACAGCTGGCACATTTAGTTTTTCAACAAGTTTAACAAAAGACGAA
  AAAGGTATTAATATGAGCCGTATAATTGAAAGTGTTGAAAAGCACTATGATAATGGTATT
  GAACTTGAATTTAATACACTTTATCAGGTGTTACGCACTTTACAAACGAATATGAAACAA
  AATGCGGCAGGCGTTGATGTTTCTGGGAAATGGTTCTTTGACCGCTATAGTCCAACCACA
  AATATTAAAGCAGTTGGTAATGCCGATGTCACTTATGGATTAGCAATTGACGGTGATAAA
  GTTACACGTAAAGAACTTACTATTGAAGCAACTGTCACAACGCTATGCCCTTGTTCAAAA
  GAAATAAGTGAGTATTCAGCGCACAACCAACGTGGTGTTGTCACTGTTAAAACATATATT
  AATAAAGATCAAAACATAGTTGATGACTATAAAAACAAAATTTTAGATGCAATGGAAGCT
  AATGCAAGTTCAATTCTATACCCTATTTTAAAACGTCCTGATGAAAAACGTGTGACAGAG
  CGTGCATATGAAAATCCACGTTTTGTCGAAGACTTAATTCGTTTAATTGCCGCGGACTTA
  GTTGAATTTGATTGGTTAGACGGTTTCGATATCGAATGTCGTAATGAAGAGTCTATCCAT
  CAACATGATGCTTTCGCTAAATTAAAGTATCGCAAATAA
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⊟Protein

⊟General

• locus tag: SA0524 [new locus tag: SA_RS03050 ]
• symbol: SA0524
• description: GTP cyclohydrolase
• length: 292
• theoretical pI: 4.93644
• theoretical MW: 33480.4
• GRAVY: -0.542466

⊟Function

• ⊞reaction:
  EC 3.5.4.16?  ExPASy
  GTP cyclohydrolase I GTP + H₂O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other GTP cyclohydrolase (TIGR00294; EC 3.5.4.-; HMM-score: 169.1)
• ⊞TheSEED  :
  • GTP cyclohydrolase I (EC 3.5.4.16) type 2
  Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  GTP cyclohydrolase I (EC 3.5.4.16) type 2
  and 1 more
  RNA Metabolism RNA processing and modification Queuosine-Archaeosine Biosynthesis  GTP cyclohydrolase I (EC 3.5.4.16) type 2
• ⊞PFAM:
  THBO-biosyn (CL0334) GCHY-1; Type I GTP cyclohydrolase folE2 (PF02649; HMM-score: 238.4)
  and 2 more
  HTH (CL0123) PadR; Transcriptional regulator PadR-like family (PF03551; HMM-score: 14.6)
  no clan defined PI31_Prot_N; PI31 proteasome regulator N-terminal (PF11566; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.7994
  • Cytoplasmic Membrane Score: 0.0034
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.1971
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005134
  • TAT(Tat/SPI): 0.00032
  • LIPO(Sec/SPII): 0.001326
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926244 NCBI
• RefSeq: NP_373777 NCBI
• UniProt: Q7A777 UniProt

⊟Protein sequence

• MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFLFEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIELEFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDKVTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQNIVDDYKNKILDAMEANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNEESIHQHDAFAKLKYRK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0524 < SA0525 < SA0526

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)