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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus Newman

locus tag: NWMN_RS14575 [old locus tag: NWMN_2536 ]

pan locus tag^?: SAUPAN006367000

symbol: NWMN_RS14575

pan gene symbol^?: aur

synonym:
product: peptidase M4 family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: NWMN_RS14575 [old locus tag: NWMN_2536 ]
symbol: NWMN_RS14575
product: peptidase M4 family protein
replicon: chromosome
strand: -
coordinates: 2790267..2791763
length: 1497
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_009641 (2790267..2791763) NCBI
BioCyc:
MicrobesOnline: see NWMN_2536

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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361
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541
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661
721
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841
901
961
1021
1081
1141
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1261
1321
1381
1441
ATGGCAGCATTAACCTTGTTGAGCACTTTATCACCAGCAGCATTAGCGATTGATTCAAAA
AATAAACCAGCTAATTCTGATATTAAATTTGAGGTGACTCAAAAGAGTGATGCGGTCAAA
GCATTAAAAGAATTGCCTAAATCCGAAAATGTAAAAAATATTTATCAAGATTACGCTGTT
ACTGATGTAAAAACTGATAAAAAAGGATTTACGCATTATACATTGCAACCGAGTGTTGAT
GGTGTTCATGCACCTGACAAAGAAGTGAAAGTACACGCAGACAAATCAGGAAAAGTCGTT
TTAATCAATGGGGATACTGATGCGAAGAAAGTAAAGCCAACGAATAAAGTGACATTAAGT
AAAGATGACGCAGCCGACAAAGCATTTAAAGCAGTTAAGATTGATAAGAATAAAGCGAAA
AATCTTAAAGATAAAGTCATTAAAGAAAACAAAGTTGAAATCGATGGTGACAGTAATAAA
TACGTTTATAATGTTGAGTTAATTACAGTGACACCAGAAATTTCACATTGGAAAGTTAAA
ATTGATGCTCAAACTGGCGAAATTTTAGAAAAAATGAACTTAGTTAAAGAAGCTGCAGAA
ACTGGTAAAGGAAAAGGTGTACTTGGCGATACAAAAGATATCAATATCAATAGTATTGAC
GGTGGATTTAGCCTAGAAGATTTAACGCATCAAGGTAAATTATCAGCATTTAGCTTTAAT
GATCAAACAGGTCAAGCAACATTGATTACTAATGAAGATGAAAACTTCGTAAAAGATGAG
CAACGTGCTGGCGTAGATGCAAATTATTACGCTAAACAAACATATGATTATTACAAAGAC
ACATTTGGTCGTGAATCATATGACAACCAAGGTAGTCCAATTGTTTCATTAACGCATGTT
AATAACTACGGTGGTCAAGATAACAGAAATAATGCCGCATGGATCGGTGACAAAATGATC
TATGGTGATGGTGATGGTCGCACATTCACAAGTTTATCGGGTGCAAATGACGTAGTAGCA
CACGAATTAACACACGGTGTGACACAAGAGACAGCGAACTTAGAATATAAGGACCAGTCA
GGCGCTCTAAATGAAAGCTTTTCAGATGTTTTTGGATACTTTGTAGATGACGAGGATTTC
TTAATGGGTGAAGATGTCTACACACCTGGAAAAGAGGGAGACGCTTTACGCAGCATGTCA
AACCCAGAACAATTTGGTCAACCAGCTCATATGAAAGACTATGTATTCACTGAAAAAGAT
AATGGTGGCGTACATACGAATTCTGGAATTCCAAATAAAGCAGCTTATAACGTGATTCAA
GCAATAGGGAAATCTAAATCAGAACAAATTTACTACCGAGCATTAACGGAATACTTAACA
AGTAATTCAAACTTCAAAGATTGTAAAGATGCATTATACCAAGCGGCTAAAGATTTATAT
GACGAGCAAACAGCTGAACAGGTGTATGAAGCATGGAATGAAGTAGGCGTGGAGTAA
60
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540
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1497

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: NWMN_RS14575 [old locus tag: NWMN_2536 ]
symbol: NWMN_RS14575
description: peptidase M4 family protein
length: 498
theoretical pI: 4.74447
theoretical MW: 54985.4
GRAVY: -0.701606

⊟Function[edit | edit source]

TIGRFAM:
TheSEED: see NWMN_2536
⊞PFAM:
Peptidase_MA (CL0126) Peptidase_M4_C; Thermolysin metallopeptidase, alpha-helical domain (PF02868; HMM-score: 177)
Peptidase_M4; Thermolysin metallopeptidase, catalytic domain (PF01447; HMM-score: 145.3)
and 4 more
PepSY (CL0320) FTP; Fungalysin/Thermolysin Propeptide Motif (PF07504; HMM-score: 49)
PepSY; Peptidase propeptide and YPEB domain (PF03413; HMM-score: 33.1)
no clan defined BCAS3; BCAS3 microtubule associated cell migration factor, C-terminal (PF12490; HMM-score: 13.2)
Flagellin_D3; Flagellin D3 domain (PF08884; HMM-score: 8)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

⊞
PSORTb: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.02
- Extracellular Score: 9.98
- Internal Helices: 0
⊞
DeepLocPro: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0.0002
- Cell wall & surface Score: 0.0083
- Extracellular Score: 0.9915
LocateP:
⊞
SignalP: Signal peptide SP(Sec/SPI) length 16 aa
- SP(Sec/SPI): 0.530122
- TAT(Tat/SPI): 0.013447
- LIPO(Sec/SPII): 0.060096
- Cleavage Site: CS pos: 16-17. ALA-ID. Pr: 0.3660
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: see NWMN_2536
RefSeq: WP_001802650 NCBI
UniProt: see NWMN_2536

⊟Protein sequence[edit | edit source]

MAALTLLSTLSPAALAIDSKNKPANSDIKFEVTQKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKVHADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKENKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGVLGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGVDANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGDGDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFLMGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVIQAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGVE

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator: CodY see NWMN_2536

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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