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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS13935 [old locus tag: SA2430 ]
  • pan locus tag?: SAUPAN006367000
  • symbol: SA_RS13935
  • pan gene symbol?: aur
  • synonym:
  • product: peptidase M4 family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS13935 [old locus tag: SA2430 ]
  • symbol: SA_RS13935
  • product: peptidase M4 family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2728001..2729497
  • length: 1497
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2728001..2729497) NCBI
  • BioCyc: SA_RS13935 BioCyc
  • MicrobesOnline: see SA2430

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCAGCAGTAACATTGTTGAGCACTTTATCACCAGCAGCATTAGCGATTGATTCAAAA
    AATAAACCAGCTAATTCTGATATTAAATTTGAGGTGACTCAAAAGAGTGATGCGGTCAAA
    GCATTAAAAGAATTGCCTAAATCCGAAAATGTAAAAAATATTTATCAAGATTACGCTGTT
    ACTGATGTAAAAACTGATAAAAAAGGATTTACGCATTATACATTGCAACCGAGTGTTGAT
    GGTGTTCATGCACCTGACAAAGAAGTGAAAGTACACGCAGACAAATCAGGAAAAGTCGTT
    TTAATCAATGGGGATACTGATGCGAAGAAAGTAAAGCCAACGAATAAAGTGACATTAAGT
    AAAGATGACGCAGCCGACAAAGCATTTAAAGCAGTTAAGATTGATAAGCATAAAGCGAAA
    AATCTTAAAGATAAAGTCATTAAAGAAAACAAAGTTGAAATCGATGGTGACAGTAATAAA
    TACGTTTATAATGTTGAGTTAATTACAGTGACACCAGAAATTTCACATTGGAAAGTTAAA
    ATTGATGCTCAAACTGGCGAAATTTTAGAAAAAATGAACTTAGTTAAAGAAGCTGCAGAA
    ACTGGTAAAGGAAAAGGTGTACTTGGCGATACAAAAGATATCAATATCAATAGTATTGAC
    GGTGGATTTAGCCTAGAAGATTTAACGCATCAAGGTAAATTATCAGCATTTAGCTTTAAT
    GATCAAACAGGTCAAGCAACATTGATTACTAATGAAGATGAAAACTTCGTAAAAGATGAG
    CAACGTGCTGGCGTAGATGCAAATTATTACGCTAAACAAACATATGATTATTACAAAGAC
    ACATTTGGTCGTGAATCATATGACAACCAAGGTAGTCCAATTGTTTCATTAACGCATGTT
    AATAACTACGGTGGTCAAGATAACAGAAATAATGCCGCATGGATTGGTGACAAAATGATC
    TATGGTGATGGTGATGGTCGCACATTCACAAGTTTATCGGGTGCAAATGACGTAGTGGCA
    CACGAATTAACACACGGTGTGACACAAGAGACTGCGAACTTAGAATATAAGGACCAGTCA
    GGCGCTCTAAATGAAAGCTTTTCAGATGTTTTTGGATACTTTGTAGATGACGAGGATTTC
    TTAATGGGTGAAGATGTCTACACACCTGGAAAAGAGGGGGACGCTTTACGCAGCATGTCA
    AACCCAGAACAATTTGGTCAACCAGCTCATATGAAAGACTATGTATTCACTGAAAAAGAT
    AATGGTGGCGTACATACGAATTCTGGAATTCCAAATAAAGCAGCTTATAACGTGATTCAA
    GCAATAGGGAAATCTAAATCAGAACAAATTTACTACCGAGCATTAACGGAATACTTAACA
    AGTAATTCAAACTTCAAAGATTGTAAAGATGCATTATACCAAGCGGCTAAAGATTTATAT
    GACGAGCAAACAGCTGAACAGGTGTATGAAGCATGGAATGAAGTAGGCGTGGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1497

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS13935 [old locus tag: SA2430 ]
  • symbol: SA_RS13935
  • description: peptidase M4 family protein
  • length: 498
  • theoretical pI: 4.78658
  • theoretical MW: 54994.4
  • GRAVY: -0.700201

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 3.4.24.29?  ExPASy
    Aureolysin Cleavage of insulin B chain with specificity similar to that of thermolysin, preferring hydrophobic P1' residues. Activates the glutamyl endopeptidase (EC 3.4.21.19) of Staphylococcus aureus
  • TIGRFAM:
  • TheSEED: see SA2430
  • ⊞PFAM:
    Peptidase_MA (CL0126) Peptidase_M4_C; Thermolysin metallopeptidase, alpha-helical domain (PF02868; HMM-score: 177)
    Peptidase_M4; Thermolysin metallopeptidase, catalytic domain (PF01447; HMM-score: 145.3)
    and 3 more
    PepSY (CL0320) FTP; Fungalysin/Thermolysin Propeptide Motif (PF07504; HMM-score: 49)
    PepSY; Peptidase propeptide and YPEB domain (PF03413; HMM-score: 33.9)
    no clan defined Flagellin_D3; Flagellin D3 domain (PF08884; HMM-score: 8.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • ⊞DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0.0064
    • Extracellular Score: 0.9934
  • LocateP:
  • ⊞SignalP: Signal peptide SP(Sec/SPI) length 16 aa
    • SP(Sec/SPI): 0.482344
    • TAT(Tat/SPI): 0.013822
    • LIPO(Sec/SPII): 0.053389
    • Cleavage Site: CS pos: 16-17. ALA-ID. Pr: 0.3389
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAAVTLLSTLSPAALAIDSKNKPANSDIKFEVTQKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKVHADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKHKAKNLKDKVIKENKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGVLGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGVDANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGDGDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFLMGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVIQAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGVE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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