PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS12120 [old locus tag: SACOL2304 ]
pan locus tag^?: SAUPAN005790000
symbol: SACOL_RS12120
pan gene symbol^?: —
• synonym:
• product: YafY family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS12120 [old locus tag: SACOL2304 ]
• symbol: SACOL_RS12120
• product: YafY family transcriptional regulator
• replicon: chromosome
• strand: +
• coordinates: 2366944..2367636
• length: 693
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2366944..2367636) NCBI
• BioCyc: SACOL_RS12120 BioCyc
• MicrobesOnline: see SACOL2304

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAATAAAGCTGAAAGGCAAAATTTAATAATTACTGCAATTCAACAAAATAAAAAAATG
  ACCGCTTTAGAATTAGCTAAATATTGCAACGTATCCAAACGCACAATTTTAAGAGATATT
  GATGATTTAGAAAATCAAGGTGTTAAAATTTATGCGCATTATGGGAAAAATGGTGGTTAC
  CAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCC
  TTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATC
  AACGCAATTATAAAACAATGTTTAAGTCTTCCACAAACACGCTTAAGAAAATTGCTTAAA
  CGCATGGACTTTTATATTAAATTTGATGACACACAACATATGACACTCCCAATGCTGTTT
  TCCGACATTTTAATTTATTGTACAGAACGAAATGTGATGTTAGTAGATCATAGGGTTGAT
  GATAATATTAAAGCTGAAAACGTTATATTTATTGGCCTTTTGTGTAAACATGGACATTGG
  CATGCAGTCATTTATGACATTGCTCAAGACAAAACTGCCGAACTCGAAATTGAAAATATT
  ATAGATATTTCGTATTCATTCGGTAAGACGATTCAAACCAGAGACATATCCATTGATAAC
  TATCATCAATTTTTAAACCCCATCGATTCCTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS12120 [old locus tag: SACOL2304 ]
• symbol: SACOL_RS12120
• description: YafY family transcriptional regulator
• length: 230
• theoretical pI: 7.17682
• theoretical MW: 26618.5
• GRAVY: -0.268261

⊟Function

• ⊞TIGRFAM:
  Unknown function General Rrf2 family protein (TIGR00738; HMM-score: 17.7)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD family protein (TIGR04548; HMM-score: 16.8)
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 15.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly SUF system regulator (TIGR02944; HMM-score: 15)
  Signal transduction Regulatory functions DNA interactions FeS assembly SUF system regulator (TIGR02944; HMM-score: 15)
  and 4 more
  Unknown function General DNA binding domain, excisionase family (TIGR01764; HMM-score: 14)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 13)
  Signal transduction Regulatory functions DNA interactions iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 13)
  Signal transduction Regulatory functions DNA interactions biotin operon repressor (TIGR00122; HMM-score: 12.1)
• TheSEED: see SACOL2304
• ⊞PFAM:
  HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 48.3)
  and 14 more
  HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 36.9)
  Rrf2; Iron-dependent Transcriptional regulator (PF02082; HMM-score: 23.3)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 21.8)
  HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 18.4)
  HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 16.8)
  Tn7_Tnp_TnsA_C; TnsA endonuclease C terminal (PF08721; HMM-score: 15.1)
  no clan defined Dicer_PBD; Dicer, partner-binding domain (PF20930; HMM-score: 14.1)
  HTH (CL0123) MarR; MarR family (PF01047; HMM-score: 13.7)
  TBPIP; TBPIP/Hop2 winged helix domain (PF07106; HMM-score: 13.6)
  HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 13.6)
  HTH_AsnC-type; AsnC-type helix-turn-helix domain (PF13404; HMM-score: 12.8)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 12.5)
  HTH_PafC; PafC helix-turn-helix domain (PF19187; HMM-score: 12.1)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8786
  • Cytoplasmic Membrane Score: 0.0728
  • Cell wall & surface Score: 0.0021
  • Extracellular Score: 0.0465
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.017591
  • TAT(Tat/SPI): 0.000918
  • LIPO(Sec/SPII): 0.002332
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446952869 NCBI
• RefSeq: WP_001030125 NCBI
• UniProt: see SACOL2304

⊟Protein sequence

• MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLENQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINAIIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVDDNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISIDNYHQFLNPIDS

⊟Experimental data

• experimentally validated: see SACOL2304
• protein localization: see SACOL2304
• quantitative data / protein copy number per cell: see SACOL2304
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see SACOL2304

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications