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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1667 [new locus tag: NWMN_RS09355 ]
  • pan locus tag?: SAUPAN004467000
  • symbol: NWMN_1667
  • pan gene symbol?: —
  • synonym:
  • product: mannosyl-glycoprotein endo-beta-N-acetylglucosamidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1667 [new locus tag: NWMN_RS09355 ]
  • symbol: NWMN_1667
  • product: mannosyl-glycoprotein endo-beta-N-acetylglucosamidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1863621..1864475
  • length: 855
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAATAAACACAAGAAAGGTTCTATTTTTGGAATAATAGGACTTGTTGTCATATTTGCT
    GTTGTCTCATTTTTATTTTTCTCAATGATATCCGATCAGATATTTTTCAAACATGTTAAA
    TCCGACATTAAGATTGAAAAGTTAAATGTTACATTAAACGATGCAGCAAAGAAACAAATA
    AATAATTATACGAGTCAACAGGTATCAAATAAAAAGAATGATGCATGGAGAGATGCATCT
    GCAACTGAAATTAAAAGTGCAATGGATAGCGGTACTTTTATCGATAATGAAAAGCAAAAA
    TATCAATTTTTAGATTTATCAAAGTATCAAGGGATTGATAAAAATAGAATTAAACGTATG
    TTAGTAGATAGACCAACGTTATTGAAACATACGGATGATTTCTTAAAAGCTGCTAAAGAT
    AAGCACGTTAACGAAGTTTATTTAATTTCACATGCATTATTAGAAACTGGCGCAGTTAAA
    AGTGAATTAGCTAATGGAGTCGAAATTGATGGCAAAAAGTACTACAATTTCTATGGAGTA
    GGAGCCCTTGATAAAGACCCAATTAAAACAGGTGCAGAATATGCTAAAAAGCATGGTTGG
    GATACACCTGAAAAAGCTATTTCAGGCGGTGCTGATTTCATTCATAAGCACTTCTTATCA
    AGCACAGATCAAAATACATTGTATAGTATGAGATGGAATCCAAAAAATCCAGGAGAACAT
    CAATATGCTACAGATATTAAGTGGGCAGAAAGTAATGCAACAATTATCGCTGACTTTTAT
    AAGAACATGAAGACTGAAGGAAAATACTTCAAATACTTTGTGTATAAAGATGACAGTAAA
    CATTTGAATAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    855

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1667 [new locus tag: NWMN_RS09355 ]
  • symbol: NWMN_1667
  • description: mannosyl-glycoprotein endo-beta-N-acetylglucosamidase
  • length: 284
  • theoretical pI: 9.78873
  • theoretical MW: 32505.8
  • GRAVY: -0.595423

⊟Function[edit | edit source]

  • TIGRFAM:
    flagellar rod assembly protein/muramidase FlgJ (TIGR02541; HMM-score: 14.8)
    Genetic information processing Protein synthesis Translation factors selenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 13.5)
  • TheSEED  :
    • Autolysin (EC 3.5.1.28)
  • ⊞PFAM:
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 51.4)
    and 1 more
    no clan defined Pox_A3L; Poxvirus A3L Protein (PF05288; HMM-score: 14.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helix: 1
  • ⊞DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0031
    • Cytoplasmic Membrane Score: 0.4794
    • Cell wall & surface Score: 0.0024
    • Extracellular Score: 0.5151
  • ⊞LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.21463
    • TAT(Tat/SPI): 0.00093
    • LIPO(Sec/SPII): 0.01466
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIKIEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQFLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVKSELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHFLSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYKDDSKHLNK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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