PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1720 [new locus tag: SAUSA300_RS09395 ]
• pan locus tag^?: SAUPAN004467000
• symbol: SAUSA300_1720
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

sagB - membrane-associated staphylococcal peptidoglycan N-acetylglucosaminidase B

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1720 [new locus tag: SAUSA300_RS09395 ]
• symbol: SAUSA300_1720
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1903091..1903945
• length: 855
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912788 NCBI
• RefSeq: YP_494413 NCBI
• BioCyc: see SAUSA300_RS09395
• MicrobesOnline: 1293235 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAATAAACACAAGAAAGGTTCTATTTTTGGAATAATAGGACTTGTTGTCATATTTGCT
  GTTGTCTCATTTTTATTTTTCTCAATGATATCCGATCAGATATTTTTCAAACATGTTAAA
  TCCGACATTAAGATTGAAAAGTTAAATGTTACATTAAACGATGCAGCAAAGAAACAAATA
  AATAATTATACGAGTCAACAGGTATCAAATAAAAAGAATGATGCATGGAGAGATGCATCT
  GCAACTGAAATTAAAAGTGCAATGGATAGCGGTACTTTTATCGATAATGAAAAGCAAAAA
  TATCAATTTTTAGATTTATCAAAGTATCAAGGGATTGATAAAAATAGAATTAAACGTATG
  TTAGTAGATAGACCAACGTTATTGAAACATACGGATGATTTCTTAAAAGCTGCTAAAGAT
  AAGCACGTTAACGAAGTTTATTTAATTTCACATGCATTATTAGAAACTGGCGCAGTTAAA
  AGTGAATTAGCTAATGGAGTCGAAATTGATGGCAAAAAGTACTACAATTTCTATGGAGTA
  GGAGCCCTTGATAAAGACCCAATTAAAACAGGTGCAGAATATGCTAAAAAGCATGGTTGG
  GATACACCTGAAAAAGCTATTTCAGGCGGTGCTGATTTCATTCATAAGCACTTCTTATCA
  AGCACAGATCAAAATACATTGTATAGTATGAGATGGAATCCAAAAAATCCAGGAGAACAT
  CAATATGCTACAGATATTAAGTGGGCAGAAAGTAATGCAACAATTATCGCTGACTTTTAT
  AAGAACATGAAGACTGAAGGAAAATACTTCAAATACTTTGTGTATAAAGATGACAGTAAA
  CATTTGAATAAGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1720 [new locus tag: SAUSA300_RS09395 ]
• symbol: SAUSA300_1720
• description: hypothetical protein
• length: 284
• theoretical pI: 9.78873
• theoretical MW: 32505.8
• GRAVY: -0.595423

⊟Function

• TIGRFAM:
  flagellar rod assembly protein/muramidase FlgJ (TIGR02541; HMM-score: 14.8)
  Genetic information processing Protein synthesis Translation factors selenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 13.5)
• TheSEED  :
  • Autolysin (EC 3.5.1.28)
• PFAM:
  Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 51.4)
  and 1 more
  no clan defined Pox_A3L; Poxvirus A3L Protein (PF05288; HMM-score: 14.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.18
  • Extracellular Score: 9.72
  • Internal Helix: 1
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0.0031
  • Cytoplasmic Membrane Score: 0.4794
  • Cell wall & surface Score: 0.0024
  • Extracellular Score: 0.5151
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.21463
  • TAT(Tat/SPI): 0.00093
  • LIPO(Sec/SPII): 0.01466
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 87161213 NCBI
• RefSeq: YP_494413 NCBI
• UniProt: A0A0H2XIC0 UniProt

⊟Protein sequence

• MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIKIEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQFLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVKSELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHFLSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYKDDSKHLNK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

Yvonne G Y Chan, Matthew B Frankel, Dominique Missiakas, Olaf Schneewind
SagB Glucosaminidase Is a Determinant of Staphylococcus aureus Glycan Chain Length, Antibiotic Susceptibility, and Protein Secretion.
J Bacteriol: 2016, 198(7);1123-36
[PubMed:26811319] [WorldCat.org] [DOI] (I e)Sara Pintar, Jure Borišek, Aleksandra Usenik, Andrej Perdih, Dušan Turk
Domain sliding of two Staphylococcus aureus N-acetylglucosaminidases enables their substrate-binding prior to its catalysis.
Commun Biol: 2020, 3(1);178
[PubMed:32313083] [WorldCat.org] [DOI] (I e)