PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS12735 [old locus tag: SACOL2427 ]
pan locus tag^?: SAUPAN005966000
symbol: SACOL_RS12735
pan gene symbol^?: bioA
• synonym:
• product: adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS12735 [old locus tag: SACOL2427 ]
• symbol: SACOL_RS12735
• product: adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA
• replicon: chromosome
• strand: -
• coordinates: 2485322..2486680
• length: 1359
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2485322..2486680) NCBI
• BioCyc: SACOL_RS12735 BioCyc
• MicrobesOnline: see SACOL2427

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAATTACACACAACAACTTAAACAAAAAGACTCAGAATATGTTTGGCATCCATTTACA
  CAAATGGGTGTATATAGCAAAGAAGAAGCAATCATCATTGAAAAAGGAAAGGGTAGTTAC
  CTTTACGATACGAATGGCAATAAATATTTAGATGGTTATGCATCGTTGTGGGTCAATGTG
  CATGGTCATAATAACAAATACTTAAATAAGGTAATTAAAAAGCAACTCAATAAAATTGCC
  CATTCTACGCTGCTAGGATCATCAAATATTCCGTCAATAGAACTTGCGGAAAAATTAATC
  GAAATCACGCCAAGTAATCTAAGAAAAGTATTTTATTCTGATACAGGCAGTGCGTCTGTT
  GAAATCGCAATAAAGATGGCATATCAGTATTGGAAAAATATTGATAGAGAAAAATATGCC
  AAGAAAAACAAGTTTATAACGCTAAATCACGGTTATCATGGGGATACGATTGGTGCGGTA
  AGTGTTGGTGGTATCAAGACCTTTCATAAAATATTTAAAGACTTAATATTTGAGAATATT
  CAAGTAGAAAGCCCATCTTTCTATCGCAGTAATTACGATACTGAAAATGAAATGATGACA
  GCTATTTTAACGAATATAGAGCAAATTCTAATTGAAAGAAATGATGAAATCGCAGGGTTT
  ATATTGGAACCGTTGATTCAAGGTGCGACAGGCTTGTTTGTTCATCCTAAAGGCTTTTTG
  AAAGAAGTCGAGAAATTGTGCAAAAAATACGATGTCTTATTAATTTGTGATGAGGTAGCA
  GTTGGTTTTGGGAGAACTGGAAAGATGTTTGCATGCAATCATGAAGATGTTCAACCGGAT
  ATTATGTGTTTAGGTAAGGCGATTACTGGTGGCTACTTACCACTTGCAGCTACATTGACA
  TCTAAAAAAATATACAATGCATTTTTAAGTGATTCGCATGGTGTGAATACCTTTTTCCAT
  GGTCATACATACACCGGAAATCAAATCGTTTGTACGGTTGCATTAGAAAATATAAGACTT
  TATGAAAAACGTAAGTTATTGTCACATATTGAAACGACATCATCAACACTTGAGAAACAG
  TTACATGCGCTGAAGCGTCATCGAAATGTTGGTGATGTAAGAGGACGAGGCTTAATGTTT
  GGAGTTGAATTAGTTACAGATAAAGATAGTAAAACGCCGTTAGAAATTGAAAAAGTTGAA
  CGTATTGTACGTAATTGTAAAGAAAACGGGCTAATGATTAGAAATTTAGAAAATGTCATT
  ACGTTTGTGCCAGTGTTAAGTATGTCAAATAAAGAAGTGAAAACGATGGTACGTATTTTT
  AAAAAGGCAGTACATAACATTTTAGATAGGAAGTGTTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS12735 [old locus tag: SACOL2427 ]
• symbol: SACOL_RS12735
• description: adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA
• length: 452
• theoretical pI: 9.20767
• theoretical MW: 51404
• GRAVY: -0.287389

⊟Function

• ⊞reaction:
  EC 2.6.1.62?  ExPASy
  Adenosylmethionine--8-amino-7-oxononanoate transaminase S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
• ⊞TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin adenosylmethionine-8-amino-7-oxononanoate transaminase (TIGR00508; EC 2.6.1.62; HMM-score: 550.6)
  and 11 more
  transaminase, acetylornithine/succinylornithine family (TIGR00707; HMM-score: 269.6)
  ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 226.6)
  Metabolism Central intermediary metabolism Other 4-aminobutyrate transaminase (TIGR00700; EC 2.6.1.19; HMM-score: 202.2)
  Metabolism Energy metabolism Amino acids and amines succinylornithine transaminase family (TIGR03246; EC 2.6.1.81; HMM-score: 201.7)
  Metabolism Central intermediary metabolism Other 2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 192.1)
  Metabolism Central intermediary metabolism Polyamine biosynthesis putrescine aminotransferase (TIGR03372; EC 2.6.1.82; HMM-score: 189.2)
  Cellular processes Cellular processes Adaptations to atypical conditions diaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 168.4)
  L-lysine 6-transaminase (TIGR03251; EC 2.6.1.36; HMM-score: 144.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamate-1-semialdehyde-2,1-aminomutase (TIGR00713; EC 5.4.3.8; HMM-score: 128.6)
  Metabolism Central intermediary metabolism Other 4-aminobutyrate aminotransferase (TIGR00699; EC 2.6.1.19; HMM-score: 99)
  Metabolism Energy metabolism Amino acids and amines glycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 12.5)
• TheSEED: see SACOL2427
• ⊞PFAM:
  PLP_aminotran (CL0061) Aminotran_3; Aminotransferase class-III (PF00202; HMM-score: 364.7)
  and 1 more
  no clan defined Pox_L5; Poxvirus L5 protein family (PF04872; HMM-score: 12.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: pyridoxal 5'-phosphate
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.7306
  • Cytoplasmic Membrane Score: 0.0667
  • Cell wall & surface Score: 0.0025
  • Extracellular Score: 0.2002
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.046625
  • TAT(Tat/SPI): 0.000523
  • LIPO(Sec/SPII): 0.00274
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447032808 NCBI
• RefSeq: WP_001110064 NCBI
• UniProt: see SACOL2427

⊟Protein sequence

• MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAVSVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC

⊟Experimental data

• experimentally validated: see SACOL2427
• protein localization: see SACOL2427
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: BirA see SACOL2427

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications