PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_2036 [new locus tag: NWMN_RS11760 ]
pan locus tag^?: SAUPAN005428000
symbol: NWMN_2036
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2036 [new locus tag: NWMN_RS11760 ]
• symbol: NWMN_2036
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2253593..2254786
• length: 1194
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331240 NCBI
• RefSeq: YP_001333070 NCBI
• BioCyc:
• MicrobesOnline: 3707629 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGACAAATAATGGTAATGAGCCGAACTTAACGTTAAGTGATTTATATGACAAAGATGTT
  GTCTATACGTCAAGACCTTCGTATATATCGAATCCATGGTTGAAACCGGACGAACATCAA
  TCTAATTTTTTAACTGGTAGGGAGTTATTAATAGCAAATCAATTACCTGTTATTGTTCAC
  GAAGCTAGTGCGACAGACAAATTGCATCAGCTATTTCAAGTTATTGGTAAGGAAGTACCG
  AACTCAATTTATACATTTAACAATCAGCAATCTTACGAAAACTTGATCAAACAATTGGCA
  CATAAGGAAAATAAAAAGATTTATTTTCAATATATTCATGATGAAACAATCTTGAATCAG
  CAATATTATGCATTAGATAAAACGTTATTTGTTGCTTTAAATAATAAAGCAAGAATACCT
  GAATGGACAAATGGGAAGTTTTTACCAAAAAGAAAAGTTGTAAAAATTGAGCAATTCGAA
  AACGAGATTAAGAATTGGGAATTCCCGTTAGTAATTAAGCCGGGAGATGATCTTCCGACA
  GCAGGTGGCTATGGCGTTATGATTTGTTATCATGATGCAGATTTACAAAAAGCGATAACG
  CGTATTAAAGAGGCAACTGCTGAAACGAATTCGCTTATTATCGAACAGAAGATTGAAGAA
  AAAGCGAATTATTGCGTTCAGTTTGCATACTCGGAATCGCTAGGCATTCAATATCTTGGT
  GCGGCAACGCAATTAACGGATAAGTACGGATTTTATAACGGAAATGAAAATACTACTAAT
  GTACCAGAGCACGTTATAGAAGCAGGTAGACAAATTATGGAAAACGGTGTGAATCAAGGA
  TTCTTTGGTGTAGCTGGTTTTGACCTACTCGTCGATGAGGATGATAACGTTTATGCGATT
  GATTTAAACTTTAGACAAAATGGTTCAACGAGCATGTTATTACTTGCTAACGAGTTGAAT
  TCAGGATATCAAAAGTTTTATAGTTATCATTCAAAAGGTGATAACACACATTTCTTCAAT
  ACGATTTTGAAATATGTCAAAGAAGGTAGTTTATACCCGTTATCTTATTATGATGGTGAT
  TGGTACGGTGAAGATAAAGTTAAATCAAGGTTTGGCTGTATTTGGCATGGTGATTCAAAA
  GAAACAGTACTGGAGAATGAACGCGCATTTTTAGCTGAACTTGAACACTATTAG
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⊟Protein

⊟General

• locus tag: NWMN_2036 [new locus tag: NWMN_RS11760 ]
• symbol: NWMN_2036
• description: hypothetical protein
• length: 397
• theoretical pI: 4.91145
• theoretical MW: 45645.7
• GRAVY: -0.537783

⊟Function

• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 11.2)
• TheSEED  :
  • Hypothetical protein SAV2132
• ⊞PFAM:
  ATP-grasp (CL0179) ATP-grasp_4; ATP-grasp domain (PF13535; HMM-score: 19.9)
  ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 18.4)
  and 4 more
  no clan defined DUF3182; Protein of unknown function (DUF3182) (PF11379; HMM-score: 15)
  Peptidase_CA (CL0125) Peptidase_C101; Peptidase family C101 (PF16218; HMM-score: 14.9)
  Bet_v_1_like (CL0209) START_2; START-like superfamily domain (PF19569; HMM-score: 14.7)
  ATP-grasp (CL0179) CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.7147
  • Cytoplasmic Membrane Score: 0.04
  • Cell wall & surface Score: 0.0023
  • Extracellular Score: 0.2431
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004537
  • TAT(Tat/SPI): 0.000646
  • LIPO(Sec/SPII): 0.000764
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222248 NCBI
• RefSeq: YP_001333070 NCBI
• UniProt: A0A0H3K9T3 UniProt

⊟Protein sequence

• MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFLTGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHKENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFENEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKIEEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENGVNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGDNTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLAELEHY

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2036 < NWMN_2037

⊟Regulation

• ⊞regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications