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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2086 [new locus tag: SAUSA300_RS11485 ]
- pan locus tag?: SAUPAN005428000
- symbol: SAUSA300_2086
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_2086 [new locus tag: SAUSA300_RS11485 ]
- symbol: SAUSA300_2086
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 2249012..2250205
- length: 1194
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3913720 NCBI
- RefSeq: YP_494732 NCBI
- BioCyc: see SAUSA300_RS11485
- MicrobesOnline: 1293601 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGACAAATAATGGTAATGAGCCGAACTTAACGTTAAGTGATTTATATGACAAAGATGTT
GTCTATACGTCAAGACCTTCGTATATATCGAATCCATGGTTGAAACCGGACGAACATCAA
TCTAATTTTTTAACTGGTAGGGAGTTATTAATAGCAAATCAATTACCTGTTATTGTTCAC
GAAGCTAGTGCGACAGACAAATTGCATCAGCTATTTCAAGTTATTGGTAAGGAAGTACCG
AACTCAATTTATACATTTAACAATCAGCAATCTTACGAAAACTTGATCAAACAATTGGCA
CATAAGGAAAATAAAAAGATTTATTTTCAATATATTCATGATGAAACAATCTTGAATCAG
CAATATTATGCATTAGATAAAACGTTATTTGTTGCTTTAAATAATAAAGCAAGAATACCT
GAATGGACAAATGGGAAGTTTTTACCAAAAAGAAAAGTTGTAAAAATTGAGCAATTCGAA
AACGAGATTAAGAATTGGGAATTCCCGTTAGTAATTAAGCCGGGAGATGATCTTCCGACA
GCAGGTGGCTATGGCGTTATGATTTGTTATCATGATGCAGATTTACAAAAAGCGATAACG
CGTATTAAAGAGGCAACTGCTGAAACGAATTCGCTTATTATCGAACAGAAGATTGAAGAA
AAAGCGAATTATTGCGTTCAGTTTGCATACTCGGAATCGCTAGGCATTCAATATCTTGGT
GCGGCAACGCAATTAACGGATAAGTACGGATTTTATAACGGAAATGAAAATACTACTAAT
GTACCAGAGCACGTTATAGAAGCAGGTAGACAAATTATGGAAAACGGTGTGAATCAAGGA
TTCTTTGGTGTAGCTGGTTTTGACCTACTCGTCGATGAGGATGATAACGTTTATGCGATT
GATTTAAACTTTAGACAAAATGGTTCAACGAGCATGTTATTACTTGCTAACGAGTTGAAT
TCAGGATATCAAAAGTTTTATAGTTATCATTCAAAAGGTGATAACACACATTTCTTCAAT
ACGATTTTGAAATATGTCAAAGAAGGTAGTTTATACCCGTTATCTTATTATGATGGTGAT
TGGTACGGTGAAGATAAAGTTAAATCAAGGTTTGGCTGTATTTGGCATGGTGATTCAAAA
GAAACAGTACTGGAGAATGAACGCGCATTTTTAGCTGAACTTGAACACTATTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_2086 [new locus tag: SAUSA300_RS11485 ]
- symbol: SAUSA300_2086
- description: hypothetical protein
- length: 397
- theoretical pI: 4.91145
- theoretical MW: 45645.7
- GRAVY: -0.537783
⊟Function[edit | edit source]
- TIGRFAM: Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 11.2)
- TheSEED :
- Hypothetical protein SAV2132
- PFAM: ATP-grasp (CL0179) ATP-grasp_4; ATP-grasp domain (PF13535; HMM-score: 19.9)Peptidase_CA (CL0125) Peptidase_C101; Peptidase family C101 (PF16218; HMM-score: 16.1)and 2 moreno clan defined DUF3182; Protein of unknown function (DUF3182) (PF11379; HMM-score: 15.9)ATP-grasp (CL0179) CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 13.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004537
- TAT(Tat/SPI): 0.000646
- LIPO(Sec/SPII): 0.000764
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFLTGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHKENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFENEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKIEEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENGVNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGDNTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLAELEHY
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CodY (repression) regulon
CodY (TF) important in Amino acid metabolism; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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