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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0650 [new locus tag: NWMN_RS03690 ]
  • pan locus tag?: SAUPAN002565000
  • symbol: NWMN_0650
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0650 [new locus tag: NWMN_RS03690 ]
  • symbol: NWMN_0650
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 730919..731377
  • length: 459
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    GTGACACATATTATTATTGATGGAGATGCTTGTCCTGTTGTTGATTCTATTATAGATTTA
    ACAACTGAGACAGGCATTTTTGTGACAATTATTCGGAGCTTCAGCCATTTTTCGAACCAA
    TTATATCCTCCACATGTATCAACATTATATGTTGATGATGGACCAGATGCAGTTGATTAC
    AAAATTGTTCAATTATCAACGAAGGATGATATCGTAGTCACTCAAGATTATGGACTCGCA
    AGTTTATTAGTCGACAAAGTCTTAATTGTCATGCATCATAATGGGAAGATATACAATTCC
    AAAAATATTCAACAACTATTAGATAAACGATATATGAATGCACAAATTAGAAAACAAGGT
    GGTCGCCACAAAGGTCCCCCACCTTTTACAAAGCAAGATCAAAAAGTGTTTGAACAATCA
    CTATTAAAAGTCATTCATCGAATTAAGGAGCTGGATTAA
    60
    120
    180
    240
    300
    360
    420
    459

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0650 [new locus tag: NWMN_RS03690 ]
  • symbol: NWMN_0650
  • description: hypothetical protein
  • length: 152
  • theoretical pI: 7.23211
  • theoretical MW: 17257.7
  • GRAVY: -0.219079

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01108093: hypothetical protein
  • ⊞PFAM:
    PIN (CL0280) DUF188; Uncharacterized BCR, YaiI/YqxD family COG1671 (PF02639; HMM-score: 137.8)
    and 1 more
    NTN (CL0052) GATase_6; Glutamine amidotransferase domain (PF13522; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9669
    • Cytoplasmic Membrane Score: 0.0298
    • Cell wall & surface Score: 0.0006
    • Extracellular Score: 0.0028
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001461
    • TAT(Tat/SPI): 0.000095
    • LIPO(Sec/SPII): 0.000234
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPPHVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKNIQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: SigB (activation) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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