From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS12325 [old locus tag: SA2146 ]
  • pan locus tag?: SAUPAN005861000
  • symbol: SA_RS12325
  • pan gene symbol?: tcaA
  • synonym:
  • product: zinc ribbon domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS12325 [old locus tag: SA2146 ]
  • symbol: SA_RS12325
  • product: zinc ribbon domain-containing protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2411587..2412969
  • length: 1383
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2411587..2412969) NCBI
  • BioCyc: SA_RS12325 BioCyc
  • MicrobesOnline: see SA2146

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAATCTTGCCCGAAGTGCGGACAACAAGCACAAGATGATGTACAGATATGTACACAA
    TGTGGACATAAATTTGATAGTCGTCAAGCATTATATAGAAAGTCAACCGATGAAGATATA
    CAAACTAATAACATCAAGATGAGAAAAATGGTGCCTTGGGCAATTGGTTTTTTCATTTTA
    ATCTTAATTATTATATTGTTCTTTTTACTAAGAAACTTCAATTCACCTGAAGCGCAAACT
    AAAATATTAGTGAATGCGATTGAGAATAATGATAAACAAAAAGTAGCAACATTATTAAGT
    ACTAAAGATAATAAAGTAGATTCTGAAGAAGCGAAAGTATACATTAACTATATCAAAGAT
    GAAGTTGGGCTTAAGCAATTTGTCAGCGACCTTAAAAATACGGTACATAAATTGAATAAG
    AGTAAGACCAGCGTAGCTTCTTATATTCAAACCAGATCTGGTCAAAATATATTACGTGTA
    AGTAAAAATGGCACGCGTTATATCTTTTTCGATAATATGAGCTTTACTGCACCTACCAAG
    CAACCAATTGTTAAGCCGAAAGAAAAAACAAAATATGAGTTTAAATCTGGTGGTAAGAAA
    AAGATGGTTATAGCTGAAGCAAATAAAGTGACGCCAATAGGTAATTTTATACTGGGGACA
    TATAGAATTCCAGCTATGAAATCAACTGAGAACGGTGATTTTGCAGGCTATTTAAAATTT
    GATTTTAGACAAAGTAATTCTGAAACGGTAGATGTTACTGAAGATTTTGAAGAAGCAAAT
    ATAACTGTAACTTTAAAAGGCGATACAAAATTAAATGATAGTTCTAAAAAAGTAACTATA
    AATGACCGTGAAATGGCATTTTCAAGTTCCAAAACGTATGGTCCATATCCACAAAATAAA
    GATATTACCATTTCAGCTTCAGGTAAAGCGAAAGGTAAAACATTTACAACACAGACGAAG
    ACGATTAAAGCCAGCGATTTAAAATACAATACAGAGATAACTTTGAATTTTGACAGTGAA
    GATATCGAAGACTATGTTGAAAAGAAAGAAAAAGAAGAAAACAGCTTGAAGAACAAATTG
    ATAGAATTCTTTGCTGGATATTCTTTAGCGAATAATGCTGCGTTTAATCAGTCAGATTTT
    GATTTTGTATCGTCATATATAAAAAAAGGATCATCTTTTTATGATGATGTAAAGAAGCGT
    GTATCTAAAGGAAGTTTAATGATGATTAGTTCACCACAAATTATAGATGCTGAAAAACAT
    GGTGATAAGATTACCGCAACCGTAAGATTAATAAATGAAAACGGTAAGCAAGTAGATAAA
    GAATATGAGCTTGAACAAGGCTCGCAAGACCGCTTGCAATTAATCAAGACATCAGAAAAA
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1383

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS12325 [old locus tag: SA2146 ]
  • symbol: SA_RS12325
  • description: zinc ribbon domain-containing protein
  • length: 460
  • theoretical pI: 9.60167
  • theoretical MW: 52130.9
  • GRAVY: -0.632826

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Sugars 5-deoxy-glucuronate isomerase (TIGR04378; EC 5.3.1.-; HMM-score: 15.6)
    Signal transduction Regulatory functions DNA interactions putative regulatory protein, FmdB family (TIGR02605; HMM-score: 13.7)
    and 1 more
    Cys-rich peptide, TIGR04165 family (TIGR04165; HMM-score: 5.5)
  • TheSEED: see SA2146
  • PFAM:
    NTF2 (CL0051) TcaA_5th; TcaA protein NTF2-like domain (PF22819; HMM-score: 82.5)
    no clan defined TcaA_2nd; TcaA protein second domain (PF22813; HMM-score: 68.8)
    and 16 more
    E-set (CL0159) TcaA_3rd_4th; TcaA protein 3rd/4th domain (PF22820; HMM-score: 34)
    Zn_Beta_Ribbon (CL0167) Zn_ribbon_3; zinc-ribbon domain (PF13248; HMM-score: 27.7)
    Zn_Ribbon_1; zinc-ribbon domain (PF13240; HMM-score: 25.9)
    UPF0547; Uncharacterised protein family UPF0547 (PF10571; HMM-score: 19.8)
    DZR; Double zinc ribbon (PF12773; HMM-score: 18.1)
    no clan defined DUF2950; Protein of unknown function (DUF2950) (PF11453; HMM-score: 15.1)
    EssA; WXG100 protein secretion system (Wss), protein EssA (PF10661; HMM-score: 14.7)
    Zn_Beta_Ribbon (CL0167) Zn_ribbon_8; Zinc ribbon domain (PF09723; HMM-score: 13.9)
    ClpP_crotonase (CL0127) CLP_protease; Clp protease (PF00574; HMM-score: 13.4)
    Zn_Beta_Ribbon (CL0167) DZR_2; Double zinc ribbon domain (PF18912; HMM-score: 12.5)
    DUF7575; Domain of unknown function (DUF7575) (PF24460; HMM-score: 12.4)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 11.4)
    Zn_Beta_Ribbon (CL0167) Zn_ribbon_15; zinc-ribbon family (PF17032; HMM-score: 11.3)
    Cas12f1-like_TNB; Cas12f1-like, TNB domain (PF07282; HMM-score: 10.8)
    RING (CL0229) Prok-RING_1; Prokaryotic RING finger family 1 (PF14446; HMM-score: 10.4)
    no clan defined YhfH; YhfH-like protein (PF14149; HMM-score: 7.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.8586
    • Cell wall & surface Score: 0.0016
    • Extracellular Score: 0.1398
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008059
    • TAT(Tat/SPI): 0.000171
    • LIPO(Sec/SPII): 0.029427
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNNIKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTKDNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRVSKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFILGTYRIPAMKSTENGDFAGYLKFDFRQSNSETVDVTEDFEEANITVTLKGDTKLNDSSKKVTINDREMAFSSSKTYGPYPQNKDITISASGKAKGKTFTTQTKTIKASDLKYNTEITLNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSSFYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQDRLQLIKTSEK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA_RS12325&oldid=95145"