PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS11350 [old locus tag: SA1970 ]
• pan locus tag^?: SAUPAN005554000
• symbol: SA_RS11350
• pan gene symbol^?: lmrS
• synonym:
• product: MFS transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS11350 [old locus tag: SA1970 ]
• symbol: SA_RS11350
• product: MFS transporter
• replicon: chromosome
• strand: -
• coordinates: 2237543..2238985
• length: 1443
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2237543..2238985) NCBI
• BioCyc: SA_RS11350 BioCyc
• MicrobesOnline: see SA1970

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGGCTAAAGTTGAATTAACAACCCGGAGACGAAACTTCATAGTTGCTGTTATGTTGATT
  AGTGCGTTTGTAGCTATTTTAAATCAAACATTATTAAATACAGCGTTACCTAGTATAATG
  AGAGAATTAAATATTAATGAAAGTACATCGCAATGGCTAGTTACTGGGTTTATGCTTGTT
  AATGGCGTCATGATACCTCTGACGGCATATCTAATGGATAGAATTAAAACTAGACCTTTA
  TACTTAGCGGCGATGGGGACATTTTTATTAGGTTCTATTGTTGCAGCCTTAGCTCCGAAT
  TTTGGAGTTTTAATGTTAGCACGTGTAATTCAAGCGATGGGTGCAGGCGTACTTATGCCC
  TTAATGCAATTTACGTTATTTACATTGTTCAGTAAAGAACATCGAGGTTTTGCAATGGGA
  CTAGCAGGTTTAGTAATTCAATTTGCACCAGCAATAGGCCCTACAGTTACAGGATTAATT
  ATTGATCAAGCGAGTTGGCGAGTACCATTTATTATAATTGTAGGAATTGCTTTAGTTGCC
  TTTGTTTTCGGCTTAGTTTCAATCTCAAGTTACAATGAAGTGAAATATACGAAATTAGAT
  AAGCGCTCAGTAATGTATTCAACTATTGGATTCGGGTTAATGCTATACGCATTTAGTAGT
  GCAGGAGATTTAGGATTTACAAGTCCAATAGTAATAGGTGCGTTGATAATAAGTATGGTT
  ATTATCTATTTATTTATACGTAGACAATTTAATATTACTAATGTACTTTTAAATTTAAGG
  GTTTTTAAAAATAGAACATTTGCATTATGTACGATTAGTTCAATGATTATAATGATGTCA
  ATGGTTGGACCTGCGCTGCTTATACCGCTATATGTTCAAAATAGTTTATCTTTATCTGCC
  TTGTTATCAGGACTTGTTATCATGCCTGGTGCAATAATAAATGGTATTATGTCAGTTTTT
  ACAGGTAAATTTTATGATAAGTATGGTCCTAGACCATTGATTTATACTGGTTTTACAATT
  TTAACAATTACTACAATTATGTTGTGTTTCTTGCATACAGACACATCTTATACGTATTTA
  ATAGTCGTATATGCAATTAGAATGTTTTCAGTTTCTTTACTCATGATGCCGATAAATACT
  ACAGGAATTAATTCTTTGAGAAATGAAGAAATCTCACATGGCACGGCTATTATGAACTTT
  GGTCGTGTAATGGCTGGTTCACTAGGCACAGCTTTAATGGTTACATTAATGAGTTTTGGT
  GCAAAAATATTTTTATCTACATCGCCATCGCATTTAACTGCAACTGAAATTAAACAGCAA
  TCCATTGCTATAGGGGTGGATATCTCATTTGCTTTTGTAGCTGTGCTTGTTATGGCAGCT
  TATGTGATAGCACTTTTTATAAGAGAACCTAAAGAAATAGAAAGTAATAGAAGGAAATTT
  TAA
  60
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  420
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  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1443
  

⊟Protein

⊟General

• locus tag: SA_RS11350 [old locus tag: SA1970 ]
• symbol: SA_RS11350
• description: MFS transporter
• length: 480
• theoretical pI: 10.3424
• theoretical MW: 52652
• GRAVY: 0.855625

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 320.1)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 320.1)
  and 8 more
  multidrug resistance protein (TIGR00880; HMM-score: 69.9)
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 59.7)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 38.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 34.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 32.9)
  Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 22.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids monocarboxylate transporter (TIGR00892; HMM-score: 15.6)
  synaptic vesicle protein SV2 (TIGR01299; HMM-score: 12.7)
• TheSEED: see SA1970
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 202.8)
  and 4 more
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 21.6)
  OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 20.8)
  MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 19.6)
  no clan defined DUF5461; Family of unknown function (DUF5461) (PF17545; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 1
  • Cell wall & surface Score: 0
  • Extracellular Score: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.166362
  • TAT(Tat/SPI): 0.133436
  • LIPO(Sec/SPII): 0.016231
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI: 447063751 NCBI
• RefSeq: WP_001141007 NCBI
• UniProt: see SA1970

⊟Protein sequence

• MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELNINESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFGVLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLIIDQASWRVPFIIIVGIALVAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAFSSAGDLGFTSPIVIGALIISMVIIYLFIRRQFNITNVLLNLRVFKNRTFALCTISSMIIMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLIYTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEISHGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISFAFVAVLVMAAYVIALFIREPKEIESNRRKF

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications