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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS09450 [old locus tag: SA1672 ]
  • pan locus tag?: SAUPAN004791000
  • symbol: SA_RS09450
  • pan gene symbol?: cspR
  • synonym:
  • product: tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS09450 [old locus tag: SA1672 ]
  • symbol: SA_RS09450
  • product: tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL
  • replicon: chromosome
  • strand: -
  • coordinates: 1908978..1909448
  • length: 471
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1908978..1909448) NCBI
  • BioCyc: SA_RS09450 BioCyc
  • MicrobesOnline: see SA1672

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGACAAATCATATCGTTTTATATCAACCAGAAATCCCAGCTAATACTGGTAACATTGCA
    CGTACGTGTGCAGGTACAAACACACATTTACATTTAATTAAACCTCTTGGATTTAGAACT
    GATGATAAAATGTTAAAAAGAGCCGGTTTAGATTATTGGGAATTTGTGAATATTACGTAT
    CATGATAGTATTGAAGCATTTTTTGAAGCCACAAATGGCGAGTATTACTTATTAACAAAA
    TTTGGTAAAAAGACTTATAGTGATTTTGATTTTTCAAATCATGACAAAGATTTTTACTTC
    ATTTTTGGAAAAGAGACGACAGGATTACCAGACTGGGTGAAAGAGAAATATCAAGACACA
    GCGTTAAGAATTCCTATGAGTGAACATATTCGTTCATTAAATTTATCAAATACTGCTGCG
    TTGTTAATTTATGAAGCGTTACGTCAACAAGATTTTCCAGGATTGAATTAA
    60
    120
    180
    240
    300
    360
    420
    471

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS09450 [old locus tag: SA1672 ]
  • symbol: SA_RS09450
  • description: tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL
  • length: 156
  • theoretical pI: 6.23354
  • theoretical MW: 18082.3
  • GRAVY: -0.449359

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.207?  ExPASy
    tRNA (cytidine34-2'-O)-methyltransferase S-adenosyl-L-methionine + cytidine34 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine34 in tRNA S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine34 in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNA(Leu)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (cytidine(34)-2'-O)-methyltransferase (TIGR00185; EC 2.1.1.207; HMM-score: 213.8)
    and 2 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification RNA methyltransferase, TrmH family, group 3 (TIGR00186; HMM-score: 42.1)
    Genetic information processing Protein synthesis tRNA and rRNA base modification RNA methyltransferase, TrmH family, group 1 (TIGR00050; EC 2.1.1.-; HMM-score: 28.2)
  • TheSEED: see SA1672
  • PFAM:
    SPOUT (CL0098) SpoU_methylase; SpoU rRNA Methylase family (PF00588; HMM-score: 113)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007599
    • TAT(Tat/SPI): 0.000353
    • LIPO(Sec/SPII): 0.002107
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKMLKRAGLDYWEFVNITYHDSIEAFFEATNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIFGKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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