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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08765 [old locus tag: SA1554 ]
  • pan locus tag?: SAUPAN004397000
  • symbol: SA_RS08765
  • pan gene symbol?: acsA
  • synonym:
  • product: acetate--CoA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08765 [old locus tag: SA1554 ]
  • symbol: SA_RS08765
  • product: acetate--CoA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1780395..1782101
  • length: 1707
  • essential: yes DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1780395..1782101) NCBI
  • BioCyc: SA_RS08765 BioCyc
  • MicrobesOnline: see SA1554

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAAAGTCGAAGTTTATAAAGGAGCGCAAGGTAAACATAACCTTAAAGATTATGAAGAA
    ACATATAATACTTTTGATTGGAAAGACGTAGAACAAGCATTTTCTTGGAGTGAAACTGGA
    AAAATGAACATGGCATATGAATGTATAGATCGCCATGTAGATCAAGGATTAGGGGATAAA
    ATAGCGTTAAATTACAAAGATGAGTACAGAAAAGAATCGTATACTTATAAAGATATGCAA
    CGGTTATCTAATAAAGCAGCGAATGTTTTGTCTGAACATGCAGAAGTTGACAAAGGTGAC
    AGAGTATTTATATTTATGTCGCGTACACCTGAACTATATTTTGCGTTGTTAGGTGTTTTA
    AAAATTGGTGCAATTGTTGGGCCGTTATTTGAAGCATTTATGGAAAAGGCAGTTGCGGAT
    AGATTAGAGAACAGTGAAGCTAAAGTGTTAATTACTAATAAGGCATTGTTACCTCGAGTA
    CCTGTAGATAAATTACCGAACTTGAAAAAAATTGTTGTCGTAGATGAGGATGTAGAAGAC
    AATTACATAGACTTCATTAGTTTGATGGAAACTGCTAGCGATGAATTTGACATTGAATGG
    TTAAAGTCGGATGATGGTTTGATTTTACATTATACATCAGGTTCTACTGGGCAACCTAAA
    GGTGTATTGCATGTTCAACAAGCAATGTTAGTGCACTATATTTCTGGAAAATATGTATTA
    GATTTACAAGAAGATGATGTTTATTGGTGTACAGCAGATCCAGGTTGGGTTACAGGAACA
    TCTTATGGTATTTTTGCACCATGGTTAAATGGCGCTACAAATTGTATAGCTGGTGGTCGC
    TTTTCACCAGAACAGTGGTATAGTATGATTGAAGATTTTAAAGTGACGATTTGGTATACG
    GCACCAACAGCTTTAAGAATGTTAATGAGTGCTGGTGACGATATTGTTGAGAAATATGAC
    TTGTCATCGTTACGTTCGATTCTATCAGTAGGTGAGCCTTTAAATCCTGAAGTTATAAAA
    TGGGCGAAAAAAGTATACGGTTTAACGGTGTTAGATACTTGGTGGATGACAGAAACAGGT
    GGACATATGATTGTTAACTATCCAACGATGGACGTCAAGCTTGGCTCAATGGGCAAACCA
    TTACCTGGTATTCAAGCTGCAATTATCGATGATGCAGGTAATGAATTACCACCAAATCGA
    ATGGGCAACCTTGCTATAAAAAAAGGCTGGCCATCAATGATGTATCGTATCTGGAAGAAT
    CCAGAAAAATATAAATCATATTTTATTGGAGACTGGTATGTATCTGGTGATTCGGCATAT
    AAAGATGAAGATGGTTACTTCTGGTTCCAAGGACGTGTTGATGATGTAATTATGACAGCT
    GGTGAACGAGTTGGACCATTTGAGGTTGAGTCTAAATTGGTTGAACACGAAGCAGTTGCC
    GAAGCAGGAATTATTGGTAAACCTGATCCGGTTCGCGGTGAAATAATTAAGGCGTTTGTT
    GCACTGAGAAAAGGATATGAACCAACAGACGAATTAAAAGAAGAAATTCGTTTATTTGTT
    AAAGAAGGTTTGTCGGCACATGCAGCACCACGTGAAATCGAATTTAAAGATAAATTACCT
    AAAACACGGTCAGGTAAAATTATGAGACGTGTATTAAAAGCTTGGGAATTAAATTTAGAT
    GCTGGGGATTTAAGTACAATGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    720
    780
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    960
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    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1707

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS08765 [old locus tag: SA1554 ]
  • symbol: SA_RS08765
  • description: acetate--CoA ligase
  • length: 568
  • theoretical pI: 4.77423
  • theoretical MW: 64390.1
  • GRAVY: -0.329577

⊟Function[edit | edit source]

  • reaction:
    EC 6.2.1.1?  ExPASy
    Acetate--CoA ligase ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
  • TIGRFAM:
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 543.1)
    and 12 more
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 305.3)
    benzoate-CoA ligase family (TIGR02262; HMM-score: 287.3)
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 197.9)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 188.5)
    amino acid adenylation domain (TIGR01733; HMM-score: 176.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 157.9)
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 157.3)
    Metabolism Transport and binding proteins Cations and iron carrying compounds (2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 139)
    Metabolism Central intermediary metabolism Other acetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 132.9)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 97.7)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 53.1)
    L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 35.5)
  • TheSEED: see SA1554
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 317.1)
    and 1 more
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 83.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018647
    • TAT(Tat/SPI): 0.000758
    • LIPO(Sec/SPII): 0.002669
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNMAYECIDRHVDQGLGDKIALNYKDEYRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRVFIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRVPVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQPKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCIAGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPLNPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDAGNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQGRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEPTDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLSTME

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA, CodY see SA1554

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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