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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08705 [old locus tag: SA1545 ]
  • pan locus tag?: SAUPAN004379000
  • symbol: SA_RS08705
  • pan gene symbol?: serA
  • synonym:
  • product: phosphoglycerate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08705 [old locus tag: SA1545 ]
  • symbol: SA_RS08705
  • product: phosphoglycerate dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1765786..1767390
  • length: 1605
  • essential: yes DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1765786..1767390) NCBI
  • BioCyc: SA_RS08705 BioCyc
  • MicrobesOnline: see SA1545

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAAGCAATTTAATGTACTCGTTGCAGATCCCATATCAAAAGATGGTATCAAAGCATTA
    TTAGATCACGAACAATTCAATGTAGATATTCAAACTGGCTTGTCCGAAGAAGCATTAATC
    AAAATTATACCTTCATACCATGCTTTAATCGTTCGTAGTCAAACTACGGTTACTGAAAAT
    ATCATAAATGCTGCTGATTCTTTAAAAGTAATCGCACGCGCCGGTGTTGGTGTAGATAAT
    ATTAATATTAATGCTGCAACATTAAAAGGTATTTTAGTTATTAATGCCCCAGATGGTAAT
    ACGATTTCAGCTACTGAACATTCACTGGCAATGTTATTATCAATGGCTCGAAATATTCCG
    CAAGCACACCAATCACTTACAAATAAAGAATGGAATCGAAATGCATTTAAAGGTACTGAG
    CTTTATCATAAAACATTAGGTGTCATTGGTGCTGGTAGAATTGGTTTAGGTGTTGCTAAA
    CGTGCGCAAAGTTTCGGAATGAAAATACTAGCTTTTGACCCTTACTTAACGGATGAAAAA
    GCAAAATCTTTAAGTATTACGAAGGCAACAGTTGATGAGATTGCCCAACATTCTGATTTC
    GTTACATTACATACACCACTAACACCTAAAACAAAAGGCTTAATTAATGCTGACTTTTTT
    GCCAAAGCAAAACCTAGTTTGCAAATAATCAATGTGGCACGTGGTGGTATTATTGATGAA
    AAGGCGCTAATAAAAGCATTAGACGAAGGACAAATTAGTCGGGCAGCTATCGATGTGTTT
    GAACATGAACCTGCAACTGATTCGCCTCTTGTTGCACATGATAAAATTATTGTTACACCT
    CATTTGGGTGCATCAACAGTCGAAGCTCAAGAAAAAGTGGCAATTTCTGTTTCAAATGAA
    ATCATCGAAATTTTAATTGATGGTACTGTAACGCATGCAGTGAATGCACCTAAAATGGAC
    TTAAGCAATATAGATGATACTGTAAAATCATTCATCAATTTAAGCCAAACAGTTGGTGAA
    TTAGCTATTCAATTAATGTACAATGCACCAAGCTCTATTAAAATTACGTACGGTGGCGAC
    TTAGCCTCTATTGATAGTAGTTTATTAACACGTACAATTATTACTCATATTTTAAAAGAT
    GATCTTGGTCCTGAAGTCAATATTATCAATGCTCTAATGTTGTTAAATCAACAACAAGTG
    ACATTAAATATTGAAAATAATAAAGCAGAGACAGGTTTTAGTAACTACTTAGAGGTAGAA
    CTATCAAACGATAGCGATTCCGTTAAAGTTGGCGCTTCTGTCTTTACAGGTTTCGGTCCA
    AGAATTGTTAGAATTAATAATTTTTCTGTAGACTTAAAGCCAAATCAATATCAAATTGTG
    TCATATCATAATGATACTCCAGGTATGGTAGGAAAAACTGGCGCATTGTTAGGTAAATAC
    AATATCAACATTGCATCTATGACTTTAGGTAGAACTGAAGCGGGCGGAGATGCGCTAATG
    ATTTTATCCGTTGATCAACCTGTTTCAAACAATATAATTGATGAACTTAAACAAGTTGGT
    GAATACAATCAAATTTTCACAACTGAATTGACGGTACAGTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1605

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS08705 [old locus tag: SA1545 ]
  • symbol: SA_RS08705
  • description: phosphoglycerate dehydrogenase
  • length: 534
  • theoretical pI: 5.30951
  • theoretical MW: 57619.4
  • GRAVY: 0.0191011

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.95?  ExPASy
    Phosphoglycerate dehydrogenase 3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 746.9)
    and 2 more
    Metabolism Energy metabolism Amino acids and amines L-serine dehydratase, iron-sulfur-dependent, beta subunit (TIGR00719; EC 4.3.1.17; HMM-score: 38.9)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis L-serine dehydratase, iron-sulfur-dependent, beta subunit (TIGR00719; EC 4.3.1.17; HMM-score: 38.9)
  • TheSEED: see SA1545
  • PFAM:
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 213.7)
    and 7 more
    2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 118.5)
    SDH_beta (CL0697) PGDH_inter; D-3-phosphoglycerate dehydrogenase intervening domain (PF19304; HMM-score: 69.3)
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 40)
    ACT_AHAS_ss; AHAS small subunit-like ACT domain (PF22629; HMM-score: 21.5)
    NADP_Rossmann (CL0063) F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 18.4)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 18.2)
    RS_preATP-grasp-like; Ribonucleotide synthetase preATP-grasp domain (PF22660; HMM-score: 14.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9592
    • Cytoplasmic Membrane Score: 0.0094
    • Cell wall & surface Score: 0.0022
    • Extracellular Score: 0.0292
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002437
    • TAT(Tat/SPI): 0.000141
    • LIPO(Sec/SPII): 0.000241
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIPSYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNININAATLKGILVINAPDGNTISATEHSLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAKRAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINADFFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKIIVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINLSQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINALMLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSVDLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPVSNNIIDELKQVGEYNQIFTTELTVQS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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