From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08375 [old locus tag: SA1488 ]
  • pan locus tag?: SAUPAN004275000
  • symbol: SA_RS08375
  • pan gene symbol?: valS
  • synonym:
  • product: valine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08375 [old locus tag: SA1488 ]
  • symbol: SA_RS08375
  • product: valine--tRNA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1693943..1696573
  • length: 2631
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1693943..1696573) NCBI
  • BioCyc: SA_RS08375 BioCyc
  • MicrobesOnline: see SA1488

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAATGAAACCAAAATATGATCCTCGTGAAGTTGAAGCGGGACGTTATGAAGAATGG
    GTAAAGAATGGTTATTTTAAACCGTCAGAAGATAAATCAAAAGAAACATATACAATTGTT
    ATCCCGCCACCAAATGTAACTGGTAAATTACATTTAGGACATGCATGGGATACGACTTTA
    CAAGATATCATTACACGTATGAAACGTATGCAAGGATACGATACGTTATACTTACCAGGT
    ATGGATCATGCTGGTATTGCGACACAGGCAAAGGTAGAAGCTAAATTAAATGAACAAGGA
    ATAACTAGATATGATCTTGGTCGTGAAAAGTTTTTAGAACAGGCATGGGATTGGAAAGAA
    GAGTATGCGTCATTTATTCGTGCGCAATGGGCTAAATTAGGTCTAGGTTTAGATTATAGT
    AGAGAACGTTTTACTTTAGATGAAGGTTTAAGTAAAGCAGTTAAAAAAGTTTTTGTTGAT
    TTATACAATAAAGGAATTATTTATCGTGGCGAACGTATTATAAATTGGGATCCTAAAGCA
    CGTACAGCTTTATCTGATATTGAAGTAATACATGAAGATGTTCAAGGTGCGTTTTATCAT
    TTTAAATATCCTTACGCTGATGGTGAAGGTTTTATTGAAATTGCAACAACAAGACCAGAA
    ACGATGTTAGGTGATACAGCGATTGTTGTTAACCCTAATGACGAACGATACAAAGATGTA
    ATCGGTAAAACTGTTATATTACCAATCGTAGGACGCGAACTGCCTATTTTAGCAGATGAG
    TATGTTGATATAGACTTCGGTTCTGGTGCTATGAAAGTGACACCAGCACATGACCCTAAT
    GATTTTGAAATTGGTCAAAGACATCAATTAGAAAATATTATCGTTATGGATGAAAATGGT
    AAAATGAACGACAAAGCGGGTAAATATGAAGGTATGGACCGTTTTGATTGTCGTAAACAG
    CTAGTTAAAGATTTAAAAGAACAAGATTTAGTTATCAAGATTGAAGATCATGTTCATTCT
    GTAGGTCATTCAGAACGATCTGGCGCTGTTGTTGAACCATATTTATCAACACAATGGTTT
    GTGCGCATGGAAGACTTAGCGAAACGTTCATTAGATAACCAAAAAACAGATGATCGTATT
    GATTTTTATCCGCAACGTTTCGAACATACATTTAACCAATGGATGGAAAATATTAGAGAT
    TGGACGATTTCAAGACAATTATGGTGGGGTCATCAAATTCCGGCTTGGTATCATAAAGAA
    ACAGGCGAAATATATGTTGGAGAAGAAGCGCCAACTGATATTGAAAATTGGCAACAAGAT
    GAAGATGTATTAGATACGTGGTTCTCAAGTGCTTTATGGCCTTTCTCTACGTTAGGTTGG
    CCTGATTTAGAAAGTGAAGACTTTAAACGATACTACCCAACAAATGCCTTAGTTACAGGT
    TACGATATTATCTTTTTCTGGGTCGCACGCATGATATTCCAAGGCTTAGAATTTACAGAT
    CGTCGTCCATTTAATGATGTATTATTACACGGTTTAGTTCGTGCTGAAGACGGGCGTAAG
    ATGAGTAAATCATTAGGTAATGGTGTGGATCCAATGGATGTTATTGACGAATACGGTGCT
    GATAGCTTGCGTTACTTCTTAGCAACAGGTTCATCTCCAGGACATGATTTAAGATACTCA
    ACTGAAAAAGTTGAGTCAGTGTGGAACTTTATCAATAAAATCTGGAATGGGGCACGTTTC
    AGTTTAATGAATATCGGTGAAGACTTTAAAGTTGAAGATATCGATTTAAGTGGTAACTTA
    TCATTAGCAGATAAATGGATTCTAACACGTTTAAATGAAACGATTGCAACAGTTACTGAT
    TTAAGTGACAAATATGAATTCGGCGAAGTTGGACGTGCATTATATAATTTCATTTGGGAT
    GATTTCTGTGATTGGTACATTGAAATGAGTAAAATTCCAATGAATAGTAATGATGAAGAA
    CAAAAACAAGTTACACGTTCAGTATTGAGTTATACTTTAGACAATATTATGAGAATGCTA
    CATCCATTCATGCCATTTGTAACAGAGAAAATATGGCAAAGTTTACCACATGAAGGTGAC
    ACAATTGTTAAAGCTTCATGGCCAGAAGTGCGTGAATCATTGATTTTTGAAGAAAGTAAA
    CAAACAATGCAACAACTTGTTGAAATCATTAAATCTGTAAGACAATCACGTGTAGAAGTA
    AATACGCCATTGTCTAAAGAAATACCTATTTTAATTCAAGCTAAAGATAAAGAAATTGAA
    ACAACTTTATCACAAAACAAAGATTATTTAATCAAATTCTGTAATCCTAGTACCTTAAAT
    ATTAGCACTGACGTGGAAATTCCTGAGAAAGCAATGACATCAGTTGTAATTGCAGGTAAA
    GTGGTATTACCATTAGAAGGGCTAATTGATATGGATAAGGAAATCAGCCGTTTGGAAAAA
    GAATTAGCTAAACTTCAAAGCGAATTAGATAGAGTAGATAAAAAGCTCTCTAATGAAAAC
    TTTGTAAGTAAAGCACCTGAAAAGGTTATAAATGAAGAAAAACGTAAAAAACAAGATTAT
    CAAGAAAAATATGATGGTGTGAAGGCAAGAATTGAACAATTAAAAGCATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2631

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS08375 [old locus tag: SA1488 ]
  • symbol: SA_RS08375
  • description: valine--tRNA ligase
  • length: 876
  • theoretical pI: 4.7353
  • theoretical MW: 101723
  • GRAVY: -0.560731

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.9?  ExPASy
    Valine--tRNA ligase ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 1183.3)
    and 6 more
    Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 424.5)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 200.1)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 197.3)
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 134.1)
    Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 31.4)
    Genetic information processing Protein synthesis tRNA aminoacylation arginine--tRNA ligase (TIGR00456; EC 6.1.1.19; HMM-score: 16.6)
  • TheSEED: see SA1488
  • PFAM:
    HUP (CL0039) tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 723.5)
    and 16 more
    DALR (CL0258) Anticodon_1; Anticodon-binding domain of tRNA (PF08264; HMM-score: 144.4)
    HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 108.8)
    tRNA_bind_arm (CL0298) Val_tRNA-synt_C; Valyl tRNA synthetase tRNA binding arm (PF10458; HMM-score: 82.6)
    HUP (CL0039) tRNA-synt_1_2; Leucyl-tRNA synthetase, Domain 2 (PF13603; HMM-score: 41.8)
    tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 34.8)
    no clan defined CCDC-167; Coiled-coil domain-containing protein 167 (PF15188; HMM-score: 15.1)
    HUP (CL0039) tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 14.4)
    tRNA-synt_1d; tRNA synthetases class I (R) (PF00750; HMM-score: 13.3)
    no clan defined DUF2408; Protein of unknown function (DUF2408) (PF10303; HMM-score: 13.3)
    ABC_tran_CTD; ABC transporter C-terminal domain (PF16326; HMM-score: 13.1)
    DASH_Spc19; Spc19 (PF08287; HMM-score: 11.3)
    DUF5082; Domain of unknown function (DUF5082) (PF16888; HMM-score: 10.8)
    DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 9.1)
    FapA; Flagellar Assembly Protein A (PF03961; HMM-score: 8.2)
    Vps51 (CL0295) COG2; COG (conserved oligomeric Golgi) complex component, COG2 (PF06148; HMM-score: 7.7)
    no clan defined IncA; IncA protein (PF04156; HMM-score: 6.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013436
    • TAT(Tat/SPI): 0.000535
    • LIPO(Sec/SPII): 0.002963
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPPNVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGITRYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVDLYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTRPETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPAHDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKIEDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFNQWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSSALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVLLHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVESVWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDKYEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHPFMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEVNTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIAGKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRKKQDYQEKYDGVKARIEQLKA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS04140aldehyde dehydrogenase  [2] (data from MRSA252)
    SA_RS0829550S ribosomal protein L21  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: T-box(Val) see SA1488

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ 2.0 2.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA_RS08375&oldid=86640"