PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS05230 [old locus tag: SA0923 ]
• pan locus tag^?: SAUPAN003284000
• symbol: SA_RS05230
• pan gene symbol^?: purM
• synonym:
• product: phosphoribosylformylglycinamidine cyclo-ligase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS05230 [old locus tag: SA0923 ]
• symbol: SA_RS05230
• product: phosphoribosylformylglycinamidine cyclo-ligase
• replicon: chromosome
• strand: +
• coordinates: 1048479..1049507
• length: 1029
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1048479..1049507) NCBI
• BioCyc: SA_RS05230 BioCyc
• MicrobesOnline: see SA0923

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
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  841
  901
  961
  1021
  
  ATGTCTAAAGCATATGAACAATCTGGTGTAAATATTCATGCTGGTTATGAAGCTGTAGAA
  AGAATGTCTAGTCATGTTAAACGTACGATGCGTAAAGAAGTTATCGGTGGTTTAGGTGGA
  TTCGGTGCTACATTTGATTTATCACAATTAAATATGACAGCGCCAGTTTTAGTTTCTGGA
  ACAGACGGAGTAGGTACGAAATTAAAACTAGCTATCGACTATGGGAAACATGATTCGATA
  GGTATCGATGCAGTTGCAATGTGTGTTAATGATATTTTAACGACAGGTGCAGAACCATTG
  TATTTTTTAGATTACATCGCTACAAATAAAGTTGTTCCTGAAGTTATTGAACAAATTGTT
  AAAGGTATTAGTGATGCATGCGTTGAAACGAATACTGCACTTATCGGTGGAGAGACTGCT
  GAAATGGGTGAAATGTATCACGAAGGTGAATATGATGTAGCCGGATTTGCTGTTGGAGCA
  GTTGAAAAGGATGACTATGTAGATGGTTCAGAAGTGAAAGAGGGACAAGTTGTTATAGGA
  CTTGCGTCAAGTGGCATTCATTCAAATGGATACAGTTTAGTGCGCAAATTAATTAATGAA
  TCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATAGATGTCTTTTTAGAA
  CCAACTAAATTATATGTCAAACCTGTACTTGCTTTAAAAAAAGAAGTTTCTATTAAGGCA
  ATGAATCATATTACTGGTGGAGGTTTTTATGAAAATATTCCACGTGCATTGCCAGCCGGA
  TATGCTGCTAGAATTGATACTACATCATTTCCAACGCCAAAAATATTTGATTGGTTACAA
  CAACAAGGCAATATAGACACAAATGAAATGTATAACATTTTTAACATGGGTATTGGCTAT
  ACGGTTATCGTTGATGAAAAAGATGCATCACGCGCTTTGAAGATTTTAGCAGAACAAAAT
  GTGGAAGCTTATCAAATTGGTCATATTGTGAAAAATGAGTCAACTGCAATTGAATTGTTG
  GGGGTATAA
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⊟Protein

⊟General

• locus tag: SA_RS05230 [old locus tag: SA0923 ]
• symbol: SA_RS05230
• description: phosphoribosylformylglycinamidine cyclo-ligase
• length: 342
• theoretical pI: 4.52335
• theoretical MW: 36988.8
• GRAVY: -0.0245614

⊟Function

• reaction:
  EC 6.3.3.1?  ExPASy
  Phosphoribosylformylglycinamidine cyclo-ligase ATP + 2-(formamido)-N¹-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine cyclo-ligase (TIGR00878; EC 6.3.3.1; HMM-score: 428.9)
  and 8 more
  hydrogenase expression/formation protein HypE (TIGR02124; HMM-score: 43.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate kinase (TIGR01379; EC 2.7.4.16; HMM-score: 41.7)
  Metabolism Energy metabolism Methanogenesis putative methanogenesis marker protein 2 (TIGR03267; HMM-score: 31.7)
  selenide, water dikinase (TIGR00476; EC 2.7.9.3; HMM-score: 24.6)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase II (TIGR01736; EC 6.3.5.3; HMM-score: 21.8)
  Unknown function Enzymes of unknown specificity AIR synthase-related protein, MSMEG_0567 C-terminal family (TIGR04050; HMM-score: 17.3)
  Unknown function Enzymes of unknown specificity AIR synthase-related protein, sll0787 family (TIGR04049; HMM-score: 16.9)
  bacteriocin-type signal sequence (TIGR01847; HMM-score: 11)
• TheSEED: see SA0923
• PFAM:
  no clan defined AIRS_C; AIR synthase related protein, C-terminal domain (PF02769; HMM-score: 129.9)
  and 1 more
  AIRS; AIR synthase related protein, N-terminal domain (PF00586; HMM-score: 59.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.025668
  • TAT(Tat/SPI): 0.024848
  • LIPO(Sec/SPII): 0.002504
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 445952956 NCBI
• RefSeq: WP_000030811 NCBI
• UniProt: see SA0923

⊟Protein sequence

• MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGATFDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYFLDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGAVEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVFLEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIFDWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDASRALKILAEQNVEAYQIGHIVKNESTAIELLGV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: PurR see SA0923

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications