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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA_RS04065 [old locus tag: SA0714 ]
- pan locus tag?: SAUPAN002676000
- symbol: SA_RS04065
- pan gene symbol?: uvrA
- synonym:
- product: excinuclease ABC subunit A
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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2821ATGAAAGAACCATCCATAGTAGTAAAAGGTGCTCGTGCGCATAACTTGAAAGATATTGAT
ATCGAACTACCTAAAAATAAATTAATTGTTATGACAGGTTTATCTGGGTCAGGTAAATCG
TCATTAGCATTCGATACTATATATGCTGAAGGACAACGACGTTATGTTGAATCATTAAGT
GCCTATGCGCGTCAATTTTTAGGCCAAATGGACAAACCAGATGTTGATACAATTGAAGGA
TTATCGCCAGCAATTTCAATAGATCAAAAAACAACAAGTAAAAATCCAAGATCAACTGTA
GCAACAGTAACAGAAATATATGATTATATACGTTTGTTATATGCACGTGTTGGTAAACCT
TACTGTCCAAATCACAATATAGAAATTGAATCGCAAACAGTACAACAAATGGTTGACCGC
ATTATGGAATTAGAGGCACGTACAAAGATTCAATTATTAGCACCTGTCATCGCTCATCGT
AAAGGTAGTCATGAAAAGCTAATCGAAGATATTGGTAAAAAAGGTTATGTACGTTTAAGA
ATCGATGGCGAAATTGTTGATGTAAATGATGTACCTACTTTAGATAAGAACAAGAATCAT
ACAATAGAAGTTGTTGTAGACCGATTAGTTGTTAAAGATGGAATTGAAACACGACTAGCT
GACTCTATAGAAACTGCGTTAGAGCTTTCAGAAGGACAATTAACAGTTGATGTCATTGAC
GGGGAAGACCTTAAGTTTTCAGAAAGCCATGCTTGTCCTATATGTGGATTTTCAATCGGA
GAGTTAGAACCAAGAATGTTTAGCTTTAACAGTCCTTTTGGTGCTTGTCCGACATGTGAT
GGCTTAGGCCAAAAGTTAACAGTCGATGTAGACTTGGTTGTTCCCGACAAAGATAAGACG
CTAAACGAAGGTGCAATAGAACCTTGGATACCGACGAGTTCTGATTTTTATCCAACATTG
TTAAAACGTGTTTGTGAAGTTTATAAAATCAATATGGATAAACCTTTTAAAAAGTTAACA
GAACGTCAACGTGATATTTTATTGTATGGTTCTGGTGACAAAGAAATTGAATTTACATTT
ACACAACGTCAAGGTGGTACTAGAAAACGAACAATGGTTTTCGAGGGTGTAGTTCCTAAT
ATAAGTAGACGATTCCATGAATCTCCTTCAGAATATACACGTGAAATGATGAGTAAATAT
ATGACCGAACTACCTTGTGAAACTTGTCATGGAAAGCGATTGAGTCGTGAAGCGTTATCT
GTTTATGTAGGTGGTTTAAATATTGGTGAAGTAGTCGAATATTCAATCAGTCAAGCGCTG
AACTATTATAAAAACATTGATTTGTCAGAACAAGATCAAGCGATTGCAAATCAAATATTG
AAAGAAATTATTTCCCGACTCACTTTTTTAAATAATGTGGGACTTGAATATTTAACGCTA
AACAGAGCTTCAGGTACACTTTCAGGTGGTGAAGCACAACGTATTCGATTGGCAACGCAA
ATTGGGTCGCGTTTGACTGGTGTCTTATATGTATTAGATGAGCCATCAATTGGACTGCAT
CAAAGAGATAATGATCGATTAATTAATACACTTAAAGAAATGAGAGATTTAGGAAATACT
TTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATA
GGGCCTGGTGCTGGTGAACATGGAGGACAAATTGTGTCTAGTGGGACACCTCAAAAGGTA
ATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGACGTA
CCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTGGAGCTAGAAGCAAC
AATCTTAAAGGGATTGATGTGGACATACCACTATCAATCATGACGGTTGTTACAGGTGTA
TCAGGTTCTGGTAAAAGCTCATTAGTAAATGAAGTATTATACAAATCATTAGCTCAAAAA
ATTAATAAATCTAAAGTAAAGCCAGGATTGTACGATAAGATTGAAGGTATTGATCAACTT
GATAAAATTATTGATATTGATCAATCACCGATAGGTAGAACGCCACGCTCTAATCCAGCA
ACATATACTGGTGTGTTTGATGATATACGTGATGTGTTTGCGCAAACAAATGAAGCTAAA
ATTCGAGGATATCAAAAAGGGCGTTTTAGTTTTAATGTAAAAGGTGGACGCTGTGAAGCT
TGTAAAGGTGACGGTATTATTAAAATTGAAATGCATTTTTTACCTGATGTTTATGTTCCT
TGTGAAGTGTGTGATGGTAAACGATATAATCGTGAGACACTAGAGGTTACTTACAAAGGT
AAAAATATTGCTGACATTTTAGAAATGACTGTTGAAGAAGCAACACAATTTTTTGAAAAT
ATTCCTAAGATTAAGCGCAAGTTACAAACACTAGTTGATGTTGGTCTTGGATACGTCACA
TTAGGTCAACAAGCTACAACGTTATCAGGTGGTGAGGCTCAACGTGTGAAACTTGCATCT
GAACTTCATAAACGTTCAACTGGTAAATCTATTTATATCCTAGACGAACTAACAACAGGG
TTACATGTTGACGATATTAGCAGATTATTAAAAGTATTAAATCGATTAGTTGAAAATGGT
GACACTGTTGTAATTATTGAACATAACCTAGATGTTATCAAAACAGCAGACTATATTATC
GACTTAGGTCCTGAAGGTGGTAGTGGCGGTGGTACTATTGTTGCGACTGGCACACCCGAA
GATATTGCTCAGACAAAGTCATCATATACAGGAAAGTATTTAAAAGAAGTACTTGAACGA
GATAAACAAAATACTGAAGATAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA_RS04065 [old locus tag: SA0714 ]
- symbol: SA_RS04065
- description: excinuclease ABC subunit A
- length: 948
- theoretical pI: 6.28065
- theoretical MW: 105383
- GRAVY: -0.380274
⊟Function[edit | edit source]
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit A (TIGR00630; EC 3.1.25.-; HMM-score: 1489.7)and 74 morethiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 110.1)Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 109.2)Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 103)Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 103)Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04520; EC 3.6.3.-; HMM-score: 100.3)Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 95.1)proposed F420-0 ABC transporter, ATP-binding protein (TIGR03873; HMM-score: 89.2)Transport and binding proteins Anions phosphate ABC transporter, ATP-binding protein (TIGR00972; EC 3.6.3.27; HMM-score: 85.3)Transport and binding proteins Cations and iron carrying compounds cobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 83.1)ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 75.8)Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 73.1)thiol reductant ABC exporter, CydC subunit (TIGR02868; HMM-score: 70.3)Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01842; HMM-score: 69.9)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 67.1)Transport and binding proteins Other lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 67.1)phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 66.9)Protein fate Protein and peptide secretion and trafficking lipoprotein releasing system, ATP-binding protein (TIGR02211; EC 3.6.3.-; HMM-score: 66.8)Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 65.3)Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikD (TIGR02770; HMM-score: 63.3)Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, ATP-binding subunit (TIGR03771; HMM-score: 63)Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikE (TIGR02769; EC 3.6.3.24; HMM-score: 61.6)Transport and binding proteins Other daunorubicin resistance ABC transporter, ATP-binding protein (TIGR01188; HMM-score: 60.5)Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 58.3)Protein fate Protein and peptide secretion and trafficking ABC-type bacteriocin transporter (TIGR01193; HMM-score: 58.3)Protein fate Protein modification and repair ABC-type bacteriocin transporter (TIGR01193; HMM-score: 58.3)Transport and binding proteins Other ABC-type bacteriocin transporter (TIGR01193; HMM-score: 58.3)Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01846; HMM-score: 57.5)Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 55.3)Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 55.3)Transport and binding proteins Anions sulfate ABC transporter, ATP-binding protein (TIGR00968; EC 3.6.3.25; HMM-score: 54.6)Cellular processes Pathogenesis type I secretion system ATPase (TIGR03375; HMM-score: 54.3)Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR03375; HMM-score: 54.3)Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, ATP-binding protein (TIGR03265; HMM-score: 54.2)Transport and binding proteins Carbohydrates, organic alcohols, and acids ABC transporter, ATP-binding subunit, PQQ-dependent alcohol dehydrogenase system (TIGR03864; HMM-score: 53.6)Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 53)Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 53)Transport and binding proteins Anions molybdate ABC transporter, ATP-binding protein (TIGR02142; EC 3.6.3.29; HMM-score: 51.9)Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly ATPase SufC (TIGR01978; HMM-score: 51.8)Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 51.2)Transport and binding proteins Carbohydrates, organic alcohols, and acids glucan exporter ATP-binding protein (TIGR01192; EC 3.6.3.42; HMM-score: 47.8)ABC transporter, permease/ATP-binding protein (TIGR02204; HMM-score: 47.7)Transport and binding proteins Other pigment precursor permease (TIGR00955; HMM-score: 46.7)D-methionine ABC transporter, ATP-binding protein (TIGR02314; EC 3.6.3.-; HMM-score: 46.4)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 45)Transport and binding proteins Other LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 45)Transport and binding proteins Anions nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 44.2)Transport and binding proteins Other nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 44.2)Energy metabolism Methanogenesis methyl coenzyme M reductase system, component A2 (TIGR03269; HMM-score: 40.4)Transport and binding proteins Amino acids, peptides and amines choline ABC transporter, ATP-binding protein (TIGR03415; HMM-score: 40.4)Transport and binding proteins Amino acids, peptides and amines polyamine ABC transporter, ATP-binding protein (TIGR01187; EC 3.6.3.31; HMM-score: 39.4)ectoine/hydroxyectoine ABC transporter, ATP-binding protein EhuA (TIGR03005; HMM-score: 38.5)Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 38)Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 38)Transport and binding proteins Carbohydrates, organic alcohols, and acids D-xylose ABC transporter, ATP-binding protein (TIGR02633; EC 3.6.3.17; HMM-score: 35.6)Central intermediary metabolism Phosphorus compounds phosphonate C-P lyase system protein PhnK (TIGR02323; HMM-score: 34.7)lantibiotic protection ABC transporter, ATP-binding subunit (TIGR03740; HMM-score: 34.4)Transport and binding proteins Other thiamine ABC transporter, ATP-binding protein (TIGR01277; EC 3.6.3.-; HMM-score: 34.1)ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 33.6)Cellular processes Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 31.1)Transport and binding proteins Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 31.1)Transport and binding proteins Other antigen peptide transporter 2 (TIGR00958; HMM-score: 29.4)gliding motility-associated ABC transporter ATP-binding subunit GldA (TIGR03522; HMM-score: 26.2)Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 22.8)2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 22.8)Central intermediary metabolism Sulfur metabolism adenylyl-sulfate kinase (TIGR00455; EC 2.7.1.25; HMM-score: 18.6)Transport and binding proteins Amino acids, peptides and amines cyclic peptide transporter (TIGR01194; HMM-score: 18.5)Transport and binding proteins Other cyclic peptide transporter (TIGR01194; HMM-score: 18.5)Transport and binding proteins Other multi drug resistance-associated protein (MRP) (TIGR00957; HMM-score: 17.5)Transport and binding proteins Anions cystic fibrosis transmembrane conductor regulator (CFTR) (TIGR01271; EC 3.6.3.49; HMM-score: 17.1)Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 17)Transport and binding proteins Carbohydrates, organic alcohols, and acids peroxysomal long chain fatty acyl transporter (TIGR00954; HMM-score: 15.7)carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 14.1)Transport and binding proteins Other pleiotropic drug resistance family protein (TIGR00956; HMM-score: 10.7)Transcription RNA processing polynucleotide kinase-phosphatase (TIGR04075; HMM-score: 10.5)
- TheSEED: see SA0714
- PFAM: P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 63.4)and 25 moreSMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 50.3)AAA_16; AAA ATPase domain (PF13191; HMM-score: 29.9)AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 27.8)RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 27.5)no clan defined DnaJ_CXXCXGXG; DnaJ central domain (PF00684; HMM-score: 23.5)P-loop_NTPase (CL0023) AAA_23; AAA domain (PF13476; HMM-score: 22.5)AAA_33; AAA domain (PF13671; HMM-score: 22)AAA_22; AAA domain (PF13401; HMM-score: 21.7)AAA_14; AAA domain (PF13173; HMM-score: 20.2)AAA_15; AAA ATPase domain (PF13175; HMM-score: 19.9)AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 19.7)APS_kinase; Adenylylsulphate kinase (PF01583; HMM-score: 19.3)ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 17.4)AAA_18; AAA domain (PF13238; HMM-score: 17.4)cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 16.5)Viral_helicase1; Viral (Superfamily 1) RNA helicase (PF01443; HMM-score: 15.6)DUF2075; Uncharacterized conserved protein (DUF2075) (PF09848; HMM-score: 15.3)AAA_24; AAA domain (PF13479; HMM-score: 15.1)RNA_helicase; RNA helicase (PF00910; HMM-score: 14.3)NACHT; NACHT domain (PF05729; HMM-score: 13.8)ATPase; KaiC (PF06745; HMM-score: 13.8)AAA_25; AAA domain (PF13481; HMM-score: 13.2)Zeta_toxin; Zeta toxin (PF06414; HMM-score: 12.1)MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 11.1)no clan defined Anti-TRAP; Tryptophan RNA-binding attenuator protein inhibitory protein (PF15777; HMM-score: 10.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.017213
- TAT(Tat/SPI): 0.005707
- LIPO(Sec/SPII): 0.003096
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVATVTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHRKGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETRLADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGACPTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDKPFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEYTREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSEQDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGVLYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGEHGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIDVPEYRRPASDRKISIRGARSNNLKGIDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDKIIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEACKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFFENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDELTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIVATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: LexA see SA0714
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
Mol Microbiol: 2002, 43(6);1387-400
[PubMed:11952893] [WorldCat.org] [DOI] (P p) - ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)