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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03160 [old locus tag: SA0546 ]
  • pan locus tag?: SAUPAN002369000
  • symbol: SA_RS03160
  • pan gene symbol?: lipL
  • synonym:
  • product: biotin/lipoate A/B protein ligase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03160 [old locus tag: SA0546 ]
  • symbol: SA_RS03160
  • product: biotin/lipoate A/B protein ligase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 639401..640165
  • length: 765
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (639401..640165) NCBI
  • BioCyc: SA_RS03160 BioCyc
  • MicrobesOnline: see SA0546

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
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    361
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    481
    541
    601
    661
    721
    ATGCAATCTTTCGCATTCGATGATACATTTTGCGAAAGTGTGGGCAAAGATATATCAGAT
    AATGTTGTGCGTACTTGGATTCATCAACATACTGTTATTCTTGGTATTCATGATTCAAGA
    TTGCCGTTTTTAAAAGATGGCATTGATTATTTAACGAATGAGATTGGTTATAATGCCATT
    GTTAGAAATTCTGGTGGCTTAGGTGTCGTTCTAGATCAAGGTGTATTAAATATATCGCTG
    ATGTTCAAAGGACAAACAGAAACAACGATTGATGAAGCGTTTACTGTGATGTACCTCTTA
    ATTAGCAAAATGTTCGAAGATGAGAATGTTGATATTGATACGATGGAAATTGAACATTCT
    TATTGCCCAGGAAAATTTGACTTAAGTATCGATGGTAAGAAATTTGCAGGCATATCGCAA
    CGAAGAGTTAGAGGCGGTATTGCTGTACAAATTTATCTTTGTGTTGAAGGCTCTGGTTCA
    GAACGTGCATTGATGATGCAAACATTTTATGAACATGCTTTAAAAGGTGAAGTGACTAAA
    TTTAAATATCCTGAAATTGAACCATCTTGTATGGCCTCATTAGAGACATTGCTTAACAAA
    ACGATTACTGTTCAAGATGTAATGTTTTTACTATTATATGCAATCAAAGATCTTGGCGGT
    GTATTAAATATGACGCCCATTACTCAAGAAGAATGGCAAAGATACGATACGTATTTTGAT
    AAAATGATTGAAAGAAACAAGAAAATGATAGACCAAATGCAATAG
    60
    120
    180
    240
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    360
    420
    480
    540
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    660
    720
    765

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS03160 [old locus tag: SA0546 ]
  • symbol: SA_RS03160
  • description: biotin/lipoate A/B protein ligase family protein
  • length: 254
  • theoretical pI: 4.5659
  • theoretical MW: 28954.1
  • GRAVY: -0.118898

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.204?  ExPASy
    Octanoyl-[GcvH]:protein N-octanoyltransferase [Glycine cleavage system H]-N6-octanoyl-L-lysine + a [lipoyl-carrier protein] = glycine cleavage system H + a [lipoyl-carrier protein]-N6-octanoyl-L-lysine
  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair lipoyltransferase and lipoate-protein ligase (TIGR00545; HMM-score: 14.5)
  • TheSEED: see SA0546
  • PFAM:
    tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 44.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00439
    • TAT(Tat/SPI): 0.00014
    • LIPO(Sec/SPII): 0.000678
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQSFAFDDTFCESVGKDISDNVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNISLMFKGQTETTIDEAFTVMYLLISKMFEDENVDIDTMEIEHSYCPGKFDLSIDGKKFAGISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASLETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQMQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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