PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS02660 [old locus tag: SA0461 ]
• pan locus tag^?: SAUPAN002244000
• symbol: SA_RS02660
• pan gene symbol^?: mfd
• synonym:
• product: transcription-repair coupling factor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS02660 [old locus tag: SA0461 ]
• symbol: SA_RS02660
• product: transcription-repair coupling factor
• replicon: chromosome
• strand: +
• coordinates: 531217..534723
• length: 3507
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (531217..534723) NCBI
• BioCyc: SA_RS02660 BioCyc
• MicrobesOnline: see SA0461

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  2041
  2101
  2161
  2221
  2281
  2341
  2401
  2461
  2521
  2581
  2641
  2701
  2761
  2821
  2881
  2941
  3001
  3061
  3121
  3181
  3241
  3301
  3361
  3421
  3481
  
  ATGACAATATTGACAACGCTTATAAAAGAAGATAATCATTTTCAAGACCTTAATCAGGTA
  TTTGGACAAGCAAACACACTAGTAACTGGTCTTTCCCCGTCAGCTAAAGTGACGATGATT
  GCTGAAAAATATGCACAAAGTAATCAACAGTTATTATTAATTACCAATAATTTATACCAA
  GCAGATAAATTAGAAACAGATTTACTTCAATTTATAGATGCTGAAGAATTGTATAAGTAT
  CCTGTGCAAGATATTATGACCGAAGAGTTTTCAACACAAAGCCCTCAACTGATGAGTGAA
  CGTATTAGAACTTTAACTGCGTTAGCTCAAGGTAAGAAAGGGTTATTTATCGTTCCTTTA
  AATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTG
  CGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTAC
  AAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGAT
  ATCTTTCCGCTAATTGGGGAACCAATCAGAATTGAGCTATTTGATACCGAAATTGATTCT
  ATTCGCGATTTTGATGTTGAAACGCAGCGTTCCAAAGATAATATTGAAGAAGTCGATATC
  ACAACTGCAAGTGATTATATCATTACTGAAGAAGTGATTAGACATCTTAAAGAAGAGTTG
  AAAACTGCATATGAAAATACAAGACCCAAAATAGATAAATCAGTGCGCAATGATCTGAAA
  GAAACGTATGAAAGCTTTAAATTATTCGAAAGTACATACTTTGATCATCAAATACTACGT
  CGTTTAGTAGCGTTTATGTATGAAACACCTTCGACAATTATTGAGTATTTCCAAAAAGAT
  GCAATCATTGCAGTTGATGAATTTAATCGTATTAAAGAAACTGAAGAAAGTTTAACAGTA
  GAGTCTGATTCGTTTATTAGCAATATTATTGAAAGTGGTAATGGATTTATAGGACAAAGT
  TTTATAAAATATGATGATTTTGAAACATTGATTGAAGGCTATCCTGTCACTTATTTTTCA
  TTATTCGCTACAACAATGCCGATAAAACTAAATCATATTATTAAATTTTCATGTAAACCT
  GTCCAACAATTTTATGGGCAATATGACATTATGCGTTCTGAATTTCAACGATATGTCAAT
  CAAAATTATCATATCGTGGTTTTGGTCGAAACTGAAACTAAAGTTGAACGTATGCAAGCG
  ATGTTAAGTGAAATGCATATTCCATCAATAACAAAATTGCATCGCTCAATGTCATCGGGG
  CAAGCAGTGATTATTGAAGGCAGTTTATCTGAAGGATTTGAACTACCTGATATGGGATTA
  GTTGTCATTACTGAGCGTGAGCTTTTTAAATCAAAACAGAAAAAGCAACGAAAACGTACG
  AAAGCTATCTCAAATGCTGAAAAAATTAAGTCTTACCAAGATTTAAATGTGGGAGATTAT
  ATTGTTCATGTGCATCATGGTGTTGGTAGATATTTAGGTGTTGAGACGCTCGAAGTGGGG
  CAAACGCATCGTGATTATATTAAATTGCAATATAAAGGTACGGATCAACTATTTGTTCCA
  GTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTA
  AATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCAAAAGTTCAACAAAGTGTTGAA
  GATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAA
  TATGGGGAAGACACAGCTGAGCAAACAACATTTGAATTAGATTTTCCATATGAACTTACG
  CCTGACCAAGCTAAATCTATCGATGAAATTAAAGATGACATGCAAAAATCGCGTCCAATG
  GATCGCTTGCTATGTGGTGATGTTGGTTATGGTAAAACTGAAGTTGCAGTGAGAGCAGCA
  TTCAAAGCTGTAATGGAAGGAAAGCAAGTTGCATTTTTAGTTCCTACAACTATTTTAGCT
  CAGCAACATTATGAGACGTTAATTGAGCGTATGCAAGATTTTCCTGTTGAAATTCAATTA
  ATGAGTCGTTTTAGAACGCCGAAAGAGATAAAACAAACTAAGGAAGGACTTAAAACTGGG
  TTTGTTGACATAGTTGTTGGTACACACAAATTACTTAGTAAAGATATACAGTATAAAGAT
  TTAGGGCTGTTGATTGTAGATGAAGAACAACGATTTGGTGTACGCCATAAAGAGCGTATT
  AAAACATTAAAACATAATGTAGATGTACTAACATTGACTGCAACCCCAATACCTAGAACA
  TTGCATATGAGTATGCTAGGTGTGCGCGATTTGTCAGTGATTGAAACGCCGCCAGAAAAT
  CGTTTCCCAGTTCAAACATATGTATTAGAACAGAACATGAGTTTTATCAAAGAAGCTTTA
  GAAAGAGAACTATCCCGTGATGGCCAAGTGTTTTATCTTTATAATAAAGTGCAATCCATT
  TATGAAAAACGAGAACAACTCCAGATGTTAATGCCAGATGCTAACATTGCAGTTGCTCAT
  GGACAAATGACAGAGCGCGATTTAGAAGAAACGATGTTAAGTTTTATCAATAATGAATAT
  GATATTTTAGTAACGACGACGATTATTGAAACAGGTGTCGATGTCCCAAATGCAAATACT
  TTGATCATTGAAGATGCAGATCGCTTTGGATTGAGTCAGTTGTATCAATTAAGAGGTCGT
  GTTGGTCGTTCAAGTCGTATTGGTTATGCATACTTCTTACATCCAGCAAATAAGGTACTA
  ACTGAGACTGCAGAAGATCGATTACAAGCGATTAAAGAATTTACGGAGTTAGGCTCAGGA
  TTTAAGATTGCGATGCGTGATTTGAACATTCGTGGTGCTGGTAATTTGTTAGGTAAACAA
  CAGCACGGCTTTATTGATACAGTTGGATTTGATTTGTACAGTCAAATGTTAGAAGAAGCT
  GTAAATGAAAAACGTGGTATTAAGGAACCAGAATCTGAGGTGCCAGAAGTCGAAGTTGAT
  TTAAACTTGGATGCATATTTGCCAACAGAATATATTGCAAATGAACAAGCTAAAATTGAA
  ATTTATAAAAAGCTACGAAAAACTGAAACATTTGATCAAATTATCGACATTAAAGATGAA
  TTAATTGATCGTTTTAATGATTATCCTGTTGAAGTAGCACGTTTGCTTGATATAGTGGAA
  ATAAAAGTACACGCATTACATTCAGGTATCACGTTGATTAAAGATAAAGGGAAAATAATT
  GATATTCATTTATCTGTAAAAGCCACTGAAAATATTGATGGCGAAGTGCTGTTCAAAGCA
  ACACAACCTTTAGGTAGAACAATGAAGGTTGGTGTTCAAAATAATGCAATGACAATTACT
  TTAACGAAACAAAATCAATGGCTTGATAGTTTGAAGTTTTTAGTTAAGTGCATTGAAGAA
  AGCATGAGAATCAGTGATGAAGCATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1860
  1920
  1980
  2040
  2100
  2160
  2220
  2280
  2340
  2400
  2460
  2520
  2580
  2640
  2700
  2760
  2820
  2880
  2940
  3000
  3060
  3120
  3180
  3240
  3300
  3360
  3420
  3480
  3507
  

⊟Protein

⊟General

• locus tag: SA_RS02660 [old locus tag: SA0461 ]
• symbol: SA_RS02660
• description: transcription-repair coupling factor
• length: 1168
• theoretical pI: 4.88536
• theoretical MW: 134301
• GRAVY: -0.368579

⊟Function

• reaction:
  EC 3.6.4.-?  ExPASy
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 1127.4)
  and 4 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 388.6)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 90.6)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 58.2)
  CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 46.3)
• TheSEED: see SA0461
• PFAM:
  no clan defined CarD_CdnL_TRCF; CarD-like/TRCF domain (PF02559; HMM-score: 110.3)
  and 8 more
  TRCF; TRCF domain (PF03461; HMM-score: 82.8)
  P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 67.5)
  Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 63.5)
  ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 33)
  no clan defined Glyco_hydro_115; Glycosyl hydrolase family 115 (PF15979; HMM-score: 16.6)
  UB2H; Bifunctional transglycosylase second domain (PF14814; HMM-score: 16.3)
  P-loop_NTPase (CL0023) IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 14.5)
  AAA_22; AAA domain (PF13401; HMM-score: 12.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006634
  • TAT(Tat/SPI): 0.001734
  • LIPO(Sec/SPII): 0.000867
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446076363 NCBI
• RefSeq: WP_000154218 NCBI
• UniProt: see SA0461

⊟Protein sequence

• MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKYAQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERIRTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGYKRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNIEEVDITTASDYIITEEVIRHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDHQILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGNGFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMRSEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLSEGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGVGRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGSEWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQAKSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQHYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKDLGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPPENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANIAVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQLYQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRGAGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTEYIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHSGITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQWLDSLKFLVKCIEESMRISDEA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications