PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS01750 [old locus tag: SA0303 ]
• pan locus tag^?: SAUPAN001238000
• symbol: SA_RS01750
• pan gene symbol^?: nanT
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS01750 [old locus tag: SA0303 ]
• symbol: SA_RS01750
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 358240..359772
• length: 1533
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (358240..359772) NCBI
• BioCyc: SA_RS01750 BioCyc
• MicrobesOnline: see SA0303

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  
  ATGAAAGAAGTTGGATTTGGCACACTAAACTGGGTTGCCGTTATCATTTATCTACTAGCT
  ATGTTGTTCATTGGCGTTTATTTTACCAAGCGCGCGAGCCAAAGTACCAATAGTTTCTTT
  ACCGCAAGTGGTCGCTTGCCATCTTGGGTAGTTGGCTTTTCAATTTATGCTACTACGTTA
  AGTGCGATTACATTTATGTCTACACCAGAGAAAGCATTTTTAACAGATTGGTCATATATC
  GCTGGTAACATTGCTATCGTCGCAATTATTCCATTACTTATTTATTTCTATGTCCCTTTC
  TTTAAAAAGTTAAAGGTAACATCTGCATATGAATATTTAGAAGCTAGATTTGGCCCTAGC
  ATACGTGTCATTGGCTCATTATTATTTGTCGTTTACCATTTAGGGCGTGTTGCAATTGTT
  ATCTACTTACCAACATTAGCAATCACATCTGTATCAGACATGAACCCTTATATCGTTGCA
  TCACTCGTAGGTTTACTATGTATTTTATATACATTTTTAGGTGGTTTCGAAGGTGTGGTT
  TGGAGTGATTTCATTCAAGGCGTCATTTTATTAGGCGGCGCTTTAGTTATTATTATTCTA
  GGTGTTATGAACATTAAAGGCGGTTTTGGCACTGTCTTTGCAGATGCGATTGAGCACAAA
  AAATTAATTAGTGCAGACAATTGGAAACTAAATACTGCGGCAGCTGCCATTCCAATTATT
  TTCCTAGGAAATATTTTCAACAACTTGTATCAATACACAGCGAGTCAAGACGTCGTGCAG
  CGTTATCAAGCTTCTGATAGTTTAAAAGAAACAAATAAATCGTTATGGACAAATGGTATC
  CTAGCTTTAATTTCAGCACCCTTATTTTATGGTATGGGTACAATGCTGTATTCATTTTAT
  ACACATGAAGCTGTTTTACCAAAAGGCTTCAATACATCATCTGTAGTGCCATATTTCATT
  TTGACTGAGATGCCACCATTTGTAGCAGGATTACTTATTGCAGCCATTTTCGCCGCTGCA
  CAGTCTACCATTTCATCTAGTTTAAATTCTATATCTGCTTGTATTTCAATCGACATTAAG
  CAACGCTTCTTCGGAAAAGATAGCGAGCGACACGAAGTTAACTTTGCACGTTTCATTATC
  ATCATTGCAGGTATTTTCGGTTTTGGAATGTCACTATACTTAATTGCTTCTAATTCAAAT
  GACTTATGGGATTTATTCTTGTTTGTAACTGGATTATTCGGTGTTCCATTAGCTGGTGTA
  TTTGCAGTTGGTATTTTCACTAAACGTACGAATACATTCGGTGTTATTTGTGGATTAATA
  TTGGGTATCATCTTTGCTTATGTCTATAATGGTGTTGGCAAAGGTAACTCACCTTTCTAT
  GTATCTACCATTTCATTTACAGTTGCTTTTGTCTTTGCTTATATACTTAGCTTCATTGTC
  CCTTCAAAACATAAAAAAGATATAACGGGATTAACAATTTTTGAAAAAGATAAACCATCA
  ACATACATTTCAAAAACGGCTACGAATAAGTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1533
  

⊟Protein

⊟General

• locus tag: SA_RS01750 [old locus tag: SA0303 ]
• symbol: SA_RS01750
• description: hypothetical protein
• length: 510
• theoretical pI: 9.43489
• theoretical MW: 55889.2
• GRAVY: 0.721765

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds transporter, solute:sodium symporter (SSS) family (TIGR00813; HMM-score: 222.7)
  and 6 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines sodium/proline symporter (TIGR02121; HMM-score: 99.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A sodium/pantothenate symporter (TIGR02119; HMM-score: 72.9)
  Metabolism Transport and binding proteins Other sodium/pantothenate symporter (TIGR02119; HMM-score: 72.9)
  probable sodium:solute symporter, VC_2705 subfamily (TIGR03648; HMM-score: 58.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids cation/acetate symporter ActP (TIGR02711; HMM-score: 30.9)
  Metabolism Transport and binding proteins Cations and iron carrying compounds multicomponent Na+:H+ antiporter (TIGR00942; HMM-score: 14.1)
• TheSEED: see SA0303
• PFAM:
  APC (CL0062) SSF; Sodium:solute symporter family (PF00474; HMM-score: 141.7)
  and 2 more
  CopD_like (CL0430) UPF0093; Uncharacterised protein family (UPF0093) (PF03653; HMM-score: 12.7)
  no clan defined LRR19-TM; Leucine-rich repeat family 19 TM domain (PF15176; HMM-score: 8.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.043374
  • TAT(Tat/SPI): 0.001183
  • LIPO(Sec/SPII): 0.007584
• predicted transmembrane helices (TMHMM): 13

⊟Accession numbers

• GI: 446588381 NCBI
• RefSeq: WP_000665727 NCBI
• UniProt: see SA0303

⊟Protein sequence

• MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASGRLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFKKLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVASLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIEHKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLWTNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIAAIFAAAQSTISSSLNSISACISIDIKQRFFGKDSERHEVNFARFIIIIAGIFGFGMSLYLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYNGVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTATNK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA, NanR* see SA0303

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications