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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS00200 [old locus tag: SA0011 ]
  • pan locus tag?: SAUPAN000021000
  • symbol: SA_RS00200
  • pan gene symbol?: metX
  • synonym:
  • product: homoserine O-acetyltransferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS00200 [old locus tag: SA0011 ]
  • symbol: SA_RS00200
  • product: homoserine O-acetyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 16114..17082
  • length: 969
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (16114..17082) NCBI
  • BioCyc: SA_RS00200 BioCyc
  • MicrobesOnline: see SA0011

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACAAATTACACAGTAGATACTTTAAATCTAGGGAAATTTATTACAGAATCTGGGGAA
    GTCATAGATAACTTGCGTTTGAGATATGAGCATGTTGGTTATCATGGACAACCATTAGTT
    GTAGTTTGTCATGCATTAACTGGCAATCATTTAACATATGGAACAGATGATTATCCGGGT
    TGGTGGCGAGAAATTATTGATGGGGGATATATACCCATTCACGATTATCAATTTTTAACA
    TTTGATGTTATTGGTAGTCCTTTCGGTTCAAGTTCACCTTTAAACGACCCTCATTTTCCT
    AAAAAATTAACATTAAGAGATATAGTCAGAGCGAATGAACGAGGTATACAAGCCCTTGGT
    TATGATAAGATTAATATTTTAATAGGGGGAAGTCTTGGAGGTATGCAAGCAATGGAACTA
    CTTTACAATCAACAGTTTGAAGTAGATAAAGCCATTATCCTTGCTGCAACAAGTCGAACA
    TCATCTTATAGTAGAGCTTTCAATGAAATTGCAAGGCAAGCCATTCATCTTGGTGGTAAG
    GAAGGTCTAAGTATTGCACGCCAATTAGGTTTTTTGACATATCGATCATCAAAAAGTTAT
    GATGAACGTTTCACGCCGGATGAAGTAGTCGCATACCAACAACATCAAGGTAATAAATTT
    AAAGAACATTTTGATTTGAATTGTTATCTGACACTGCTAGATGTATTGGATAGTCACAAC
    ATTGACCGAGGTCGCACAGACGTAACGCATATTTTTAAAAATTTAGAAACGAAAGTCTTA
    ACGATGGGGTTCATAGATGATTTGCTATATCCGGACGATCAAGTTCGTGCATTAGGTGAA
    CGTTTTAAATATCATCGTCATTTCTTCGTGCCTGATAATGTTGGACATGATGGATTCCTA
    CTAAACTTTAGTACCTGGGCACCTAACTTATATCATTTCTTAAATTTAAAGCATTTTAAG
    CGTAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS00200 [old locus tag: SA0011 ]
  • symbol: SA_RS00200
  • description: homoserine O-acetyltransferase
  • length: 322
  • theoretical pI: 6.84019
  • theoretical MW: 36940.5
  • GRAVY: -0.349379

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family homoserine O-acetyltransferase (TIGR01392; EC 2.3.1.31; HMM-score: 200.9)
    and 4 more
    pyrimidine utilization protein D (TIGR03611; HMM-score: 17.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 16.6)
    proline-specific peptidase (TIGR01250; HMM-score: 16.3)
    Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 11.5)
  • TheSEED: see SA0011
  • PFAM:
    AB_hydrolase (CL0028) Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 59.4)
    and 3 more
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 31.3)
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 14)
    UPF0227; Uncharacterised protein family (UPF0227) (PF05728; HMM-score: 12.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.021661
    • TAT(Tat/SPI): 0.000273
    • LIPO(Sec/SPII): 0.000902
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTNYTVDTLNLGKFITESGEVIDNLRLRYEHVGYHGQPLVVVCHALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKKLTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRTSSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGNKFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHIFKNLETKVLTMGFIDDLLYPDDQVRALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: S-box, CodY see SA0011

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]