PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS13230 [old locus tag: SAUSA300_2390 ]
• pan locus tag^?: SAUPAN005997000
• symbol: SAUSA300_RS13230
• pan gene symbol^?: opuCD
• synonym:
• product: amino acid ABC transporter permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS13230 [old locus tag: SAUSA300_2390 ]
• symbol: SAUSA300_RS13230
• product: amino acid ABC transporter permease
• replicon: chromosome
• strand: -
• coordinates: 2573618..2574313
• length: 696
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2573618..2574313) NCBI
• BioCyc: SAUSA300_RS13230 BioCyc
• MicrobesOnline: see SAUSA300_2390

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGAAGGTAATTTATTACAGCAATTATTCAATTATTATGTTACGAACTTTGGTTATCTA
  TGGGATTTATTTTTCAAACACTTATTAATGTCTGTCTATGGTGTGCTGTTTGCAGCTTTA
  ATTGGTATTCCATTGGGAATCTTGCTTGCAAGATACACAAAACTTTCTGGATTTGTAATT
  ACAATTGCAAATATAATTCAAACAGTTCCAGTCATTGCAATGTTAGCTATTTTAATGTTA
  GTCATGGGCTTAGGTTCAGAAACAGTAGTTTTAACAGTGTTTTTATATGCGTTACTTCCA
  ATTATAAAAAACACTTATACTGGTATAGCTAGTGTTGATGCGAATATTAAGGATGCTGGC
  AAAGGTATGGGAATGACACGCAATCAAGTGCTACGAATGATTGAATTACCGTTATCTGTT
  TCGGTTATTATCGGTGGCATTCGTATTGCCTTGGTTGTTGCGATAGGTGTTGTTGCCGTT
  GGATCATTTATAGGAGCACCTACGCTTGGTGACATTGTGATTCGTGGTACAAATGCGACG
  GATGGCACAACGTTTATTTTAGCAGGTGCGATTCCGATTGCTATCATTGCAATCGTCATT
  GATGTACTATTAAGATTTTTAGAAAAACGATTAGACCCAACAACACGACATCGTAAAAAT
  CAATCTAATCATCGGCCGCAAAGTATTAATATGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS13230 [old locus tag: SAUSA300_2390 ]
• symbol: SAUSA300_RS13230
• description: amino acid ABC transporter permease
• length: 231
• theoretical pI: 10.1502
• theoretical MW: 25025.9
• GRAVY: 0.844156

⊟Function

• reaction:
  EC 3.6.3.32?  ExPASy
  Quaternary-amine-transporting ATPase ATP + H₂O + quaternary amine(Out) = ADP + phosphate + quaternary amine(In)
• TIGRFAM:
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 86.1)
  and 10 more
  Metabolism Transport and binding proteins Anions nitrate ABC transporter, permease protein (TIGR01183; HMM-score: 44.7)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 32.3)
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 31.7)
  NifC-like ABC-type porter (TIGR01581; HMM-score: 22)
  2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 19.6)
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 18.4)
  Cellular processes Cellular processes Pathogenesis autotransporter-associated beta strand repeat (TIGR02601; HMM-score: 10)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking autotransporter-associated beta strand repeat (TIGR02601; HMM-score: 10)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 9.6)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides celllulose biosynthesis operon protein BcsF/YhjT (TIGR03493; HMM-score: 5.4)
• TheSEED: see SAUSA300_2390
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 78.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 5
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001453
  • TAT(Tat/SPI): 0.000164
  • LIPO(Sec/SPII): 0.001504
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 446321086 NCBI
• RefSeq: WP_000398941 NCBI
• UniProt: see SAUSA300_2390

⊟Protein sequence

• MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIPLGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPIIKNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAVGSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHRKNQSNHRPQSINM

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SAUSA300_2390

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications