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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS10075 [old locus tag: SAUSA300_1845 ]
- pan locus tag?: SAUPAN004844000
- symbol: SAUSA300_RS10075
- pan gene symbol?: gsaB
- synonym:
- product: glutamate-1-semialdehyde 2,1-aminomutase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS10075 [old locus tag: SAUSA300_1845 ]
- symbol: SAUSA300_RS10075
- product: glutamate-1-semialdehyde 2,1-aminomutase
- replicon: chromosome
- strand: +
- coordinates: 2005499..2006788
- length: 1290
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (2005499..2006788) NCBI
- BioCyc: SAUSA300_RS10075 BioCyc
- MicrobesOnline: see SAUSA300_1845
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1261ATGAATTTTAGTGAAAGTGAACGTTTACAACAACTTTCAAACGAATATATTCTAGGCGGT
GTCAATTCCCCTTCTCGTTCTTATAAAGCTGTAGGAGGCGGTGCACCTGTTGTTATGAAA
GAAGGACACGGTGCATATTTATATGATGTCGATGGCAATAAATTTATTGATTACCTTCAA
GCATACGGTCCAATTATTACGGGGCATGCACATCCTCATATTACTAAAGCAATTCAAGAA
CAAGCTGCTAAAGGTGTTTTATTTGGTACACCGACTGAATTAGAAATTGAATTCAGCAAA
AAATTACGTGATGCAATTCCATCTCTTGAGAAAATTCGCTTTGTAAATTCTGGAACAGAA
GCAGTCATGACAACAATTCGTGTTGCACGTGCATATACTAAAAGAAATAAAATTATAAAA
TTTGCTGGATCTTATCATGGCCATTCTGATTTAGTATTGGTTGCAGCAGGTAGCGGCCCA
TCTCAGCTCGGTTCTCCAGACTCAGCTGGTGTTCCAGAAAGCGTCGCACGTGAAGTCATT
ACTGTACCTTTCAATGATATTAACGCCTATAAAGAAGCAATTGAATTTTGGGGTGATGAA
ATTGCCGCAGTATTAGTAGAACCAATTGTTGGTAACTTTGGAATGGTAATGCCTCAACCT
GGATTTTTAGAAGAGGTTAATGAAATTTCACATAACAATGGGACCCTAGTGATTTATGAT
GAAGTAATTACTGCATTCCGTTTCCATTACGGTGCCGCTCAAGATTTATTAGGTGTTATC
CCTGATTTAACTGCATTTGGTAAAATTGTTGGCGGTGGTTTACCAATTGGAGGCTATGGT
GGACGTCAAGATATTATGGAACAAGTAGCACCTCTAGGACCTGCATATCAAGCTGGTACA
ATGGCTGGTAACCCGTTATCTATGAAAGCAGGTATTGCATTACTCGAAGTACTAGAACAA
GACGGTGTTTATGAAAAATTAGACAGCTTAGGCCAACAACTAGAAGAAGGTTTACTTAAA
TTAATCGAAAAACATAATATCACAGCTACAATTAATCGTATTTATGGATCTTTAACATTG
TACTTTACAGATGAAAAAGTCACACATTATGATCAAGTTGAACATTCTGACGGCGAAGCG
TTCGGTAAATTTTTCAAATTAATGTTAAATCAAGGTATCAATTTAGCACCTTCTAAGTTT
GAAGCTTGGTTCTTAACAACTGAACATACAGAAGAAGATATTAAACAAACTTTAAAAGCT
GCAGACTATGCTTTTAGTCAAATGAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS10075 [old locus tag: SAUSA300_1845 ]
- symbol: SAUSA300_RS10075
- description: glutamate-1-semialdehyde 2,1-aminomutase
- length: 429
- theoretical pI: 4.90976
- theoretical MW: 46755.7
- GRAVY: -0.12028
⊟Function[edit | edit source]
- reaction: EC 5.4.3.8? ExPASyGlutamate-1-semialdehyde 2,1-aminomutase (S)-4-amino-5-oxopentanoate = 5-aminolevulinate
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamate-1-semialdehyde-2,1-aminomutase (TIGR00713; EC 5.4.3.8; HMM-score: 547.9)and 10 moretransaminase, acetylornithine/succinylornithine family (TIGR00707; HMM-score: 175.2)Central intermediary metabolism Other 4-aminobutyrate transaminase (TIGR00700; EC 2.6.1.19; HMM-score: 159.2)ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 155.8)Energy metabolism Amino acids and amines succinylornithine transaminase family (TIGR03246; EC 2.6.1.81; HMM-score: 133.3)Biosynthesis of cofactors, prosthetic groups, and carriers Biotin adenosylmethionine-8-amino-7-oxononanoate transaminase (TIGR00508; EC 2.6.1.62; HMM-score: 129.4)Central intermediary metabolism Polyamine biosynthesis putrescine aminotransferase (TIGR03372; EC 2.6.1.82; HMM-score: 111.8)Central intermediary metabolism Other 2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 108.3)Cellular processes Adaptations to atypical conditions diaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 87.1)L-lysine 6-transaminase (TIGR03251; EC 2.6.1.36; HMM-score: 64.9)Central intermediary metabolism Other 4-aminobutyrate aminotransferase (TIGR00699; EC 2.6.1.19; HMM-score: 43.5)
- TheSEED: see SAUSA300_1845
- PFAM: PLP_aminotran (CL0061) Aminotran_3; Aminotransferase class-III (PF00202; HMM-score: 242.3)and 1 moreMACRO (CL0223) 2CompARG; 2-Component system ADP-ribose glycohydrolase domain (PF22545; HMM-score: 14.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: pyridoxal 5'-phosphate
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9936
- Cytoplasmic Membrane Score: 0.0008
- Cell wall & surface Score: 0.0001
- Extracellular Score: 0.0055
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.038389
- TAT(Tat/SPI): 0.009961
- LIPO(Sec/SPII): 0.004266
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446934342 NCBI
- RefSeq: WP_001011598 NCBI
- UniProt: see SAUSA300_1845
⊟Protein sequence[edit | edit source]
- MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHGAYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKLRDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGPSQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMPQPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPIGGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQLEEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQGINLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_RS00585 peptidoglycan-binding protein LysM [1] (data from MRSA252) SAUSA300_RS02850 elongation factor Tu [1] (data from MRSA252) SAUSA300_RS04080 aldehyde dehydrogenase [1] (data from MRSA252) SAUSA300_RS04095 2,3-bisphosphoglycerate-independent phosphoglycerate mutase [1] (data from MRSA252) SAUSA300_RS07430 DNA-binding protein HU [1] (data from MRSA252) SAUSA300_RS11805 Asp23/Gls24 family envelope stress response protein [1] (data from MRSA252) SAUSA300_RS12055 50S ribosomal protein L15 [1] (data from MRSA252) SAUSA300_RS12090 50S ribosomal protein L5 [1] (data from MRSA252) SAUSA300_RS12120 30S ribosomal protein S3 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)