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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS08585 [old locus tag: SAUSA300_1575 ]
  • pan locus tag?: SAUPAN004215000
  • symbol: SAUSA300_RS08585
  • pan gene symbol?: alaS
  • synonym:
  • product: alanine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS08585 [old locus tag: SAUSA300_1575 ]
  • symbol: SAUSA300_RS08585
  • product: alanine--tRNA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1722056..1724686
  • length: 2631
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAAAAGTTAAAAGCGAGTGAAATTAGACAAAAATATCTAGATTTCTTTGTTGAAAAA
    GGACATATGGTTGAACCTTCTGCACCATTAGTGCCAATTGATGATGATACATTATTATGG
    ATTAATTCAGGTGTAGCAACATTAAAGAAATATTTTGATGGACGTGAAACACCTAAAAAG
    CCAAGAATTGTAAACTCTCAAAAAGCTATTCGTACAAATGATATTGAAAATGTTGGTTTC
    ACAGCGCGTCACCATACATTCTTTGAAATGTTAGGTAACTTCTCAATTGGTGATTATTTT
    AAACAAGAAGCGATTGAATTTGCTTGGGAATTTTTAACGAGTGATAAATGGATGGGTATG
    GAGCCAGATAAATTGTACGTTACGATTCATCCGGAAGATATGGAAGCATACAACATTTGG
    CATAAAGATATTGGGCTTGAAGAAAGTCGTATTATTCGCATTGAAGGTAACTTCTGGGAT
    ATTGGTGAAGGGCCTTCAGGACCGAACACTGAGATTTTCTATGATCGCGGAGAAGCATAT
    GGACAAGACGATCCGGCAGAAGAAATGTATCCAGGTGGAGAAAATGAACGCTATCTTGAA
    GTATGGAACTTAGTATTTAGTGAATTCAATCATAATAAAGATCATAGTTACACACCATTA
    CCTAATAAAAATATTGATACTGGCATGGGGCTTGAGCGTATGGCCTCAGTTTCTCAAAAT
    GTACGTACTAACTATGAAACAGATTTATTTATGCCTATAATGAATGAAATCGAAAAAGTA
    TCAGGTAAACAATATTTAGTAAACAACGAACAAGATGTGGCATTTAAAGTAATTGCTGAC
    CACATTCGTACGATTGCATTTGCAATTTCTGATGGTGCATTACCTGCCAATGAAGGTAGA
    GGGTATGTATTACGTCGATTGTTACGTCGTGCCGTTCGTTTTAGTCAAACGTTAGGAATC
    AATGAGCCATTTATGTACAAACTTGTTGATATTGTTGCAGACATTATGGAACCATATTAT
    CCAAATGTTAAGGAAAAAGCAGATTTCATTAAGCGTGTTATAAAGTCTGAAGAAGAACGA
    TTCCATGAAACATTAGAAGATGGTTTAGCGATTTTAAATGAATTAATTAAAAAAGCTAAA
    GCGACAACAAATGAAATTAATGGGAAAGATGCATTTAAATTGTATGATACGTATGGGTTC
    CCAATTGAATTAACTGAAGAAATAGCAGTGCAAGCAGGATTGAAAGTTGATATGACAACA
    TTCGAGTCAGAAATGCAACAACAACGTGATCGTGCACGTCAAGCACGTCAAAATTCTCAA
    TCAATGCAAGTTCAAAGTGAAGTATTGAAAAATATTACATCTGCAAGTACTTTTGTTGGT
    TATGATACTGCGACAGCTCAAACAACACTAACACACTTGATATACAATGGTGAAGAAGTT
    TCACAAGTTGAAGCGGGTGAAACAGTATACTTCATGTTAACGGAAACACCATTTTATGCA
    ATCAGTGGTGGACAAGTTGCGGATACAGGTATTGTTTATAATGACAATTTTGAAATTGCT
    GTTAGTGAAGTAACCAAAGCACCAAATGGTCAAAACTTGCATAAAGGAGTAGTACAATTT
    GGCCAAGTAAATGTTGGCGCTACAGTGTCTGCTGAAGTGAACCAAAATGATCGACGTGAC
    ATTCAAAAGAACCATAGTGCAACACATTTATTACATGCAGCGTTGAAATCAGTACTGGGT
    GATCATGTTAACCAAGCTGGTTCACTAGTAGAAGCAGATCGTTTACGTTTTGATTTCTCT
    CATTTTGGTCCAATGACTAATGATGAAATTGATCAAGTTGAACGCTTAGTAAATGAAGAA
    ATTTGGAAAGGTATTGACGTTAACATTCAAGAAATGGATATTGCTTCAGCTAAAGAAATG
    GGCGCAATGGCATTATTCGGTGAAAAATATGGTGATGTTGTGCGTGTAGTAAATATGGCA
    CCATTTTCAATTGAATTATGTGGTGGTATTCATGTCCGCAATACTTCTGAAATTGGCTTA
    TTCAAAATAGTAAGTGAGTCAGGTACAGGAGCTGGTGTGCGTCGTATTGAAGCATTAACA
    GGTAAAGCAGCTTTCTTATATTTAGAAGATATTCAAGAGAAATTTAATACGATGAAATCA
    CAGCTGAAAGTGAAATCTGATGATCAAGTAGTCGATAAGTTAACACAATTACAAGATGAA
    GAAAAAGCATTATTAAAACAATTAGAGCAACGTGACAAAGAAATCACATCACTTAAAATG
    GGTAATATTGAAGATCAAGTTGAAGAAATCAATGGCTATAAAGTATTGGTTACTGAAGTG
    GATGTACCAAATGCGAAAGCAATTCGCTCGACAATGGACGATTTTAAATCTAAACTACAA
    GATACAATTATCATTCTTGCAAGTAATGTTGATGATAAAGTATCGATGGTTGCAACTGTC
    CCTAAATCTTTAACAAATAACGTTAAAGCCGGTGATCTTATCAAACAAATGGCACCAATC
    GTTGGTGGTAAAGGTGGCGGTCGTCCAGATATGGCTCAAGGTGGCGGTACACAACCTGAA
    AATATCTCAAAATCATTAAGCTTTATTAAAGATTACATTAAAAATCTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2631

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS08585 [old locus tag: SAUSA300_1575 ]
  • symbol: SAUSA300_RS08585
  • description: alanine--tRNA ligase
  • length: 876
  • theoretical pI: 4.75077
  • theoretical MW: 98519.6
  • GRAVY: -0.418379

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.7?  ExPASy
    Alanine--tRNA ligase ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation alanine--tRNA ligase (TIGR00344; EC 6.1.1.7; HMM-score: 1065.1)
    and 2 more
    alanine--tRNA ligase (TIGR03683; EC 6.1.1.7; HMM-score: 421.9)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides clan AA aspartic protease, TIGR02281 family (TIGR02281; EC 3.4.23.-; HMM-score: 12.1)
  • TheSEED: see SAUSA300_1575
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2c; tRNA synthetases class II (A) (PF01411; HMM-score: 699.4)
    and 6 more
    no clan defined DHHA1; DHHA1 domain (PF02272; HMM-score: 68.5)
    Peptidase_ME (CL0094) tRNA_SAD; Threonyl and Alanyl tRNA synthetase second additional domain (PF07973; HMM-score: 66.2)
    PF (CL0431) Longin; Regulated-SNARE-like domain (PF13774; HMM-score: 12.5)
    TPR (CL0020) NELF-A_N; NELF-A N-terminal domain (PF23553; HMM-score: 11.7)
    no clan defined LRS4; Monopolin complex subunit LRS4 (PF10422; HMM-score: 10.3)
    SieB; Super-infection exclusion protein B (PF14163; HMM-score: 8.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9974
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0025
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003825
    • TAT(Tat/SPI): 0.000434
    • LIPO(Sec/SPII): 0.000469
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSGVATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQEAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWDIGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYTPLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFKVIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVADIMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAFKLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLKNITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVADTGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHSATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKGIDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFKIVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDEEKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSKLQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGGTQPENISKSLSFIKDYIKNL

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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