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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS08460 [old locus tag: SAUSA300_1552 ]
  • pan locus tag?: SAUPAN004179000
  • symbol: SAUSA300_RS08460
  • pan gene symbol?: —
  • synonym:
  • product: haloacid dehalogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS08460 [old locus tag: SAUSA300_1552 ]
  • symbol: SAUSA300_RS08460
  • product: haloacid dehalogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 1702788..1703372
  • length: 585
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAACATTGAAAAAGCAAAACGGCTTGCAAAAGAGAAACTGCCAGAGAAACGTTATAAT
    CATTCTTTAAGAGTTGCAGAAACAGCGATTAAATTGGCAGAAATTTATGATGGAGACACT
    AGTAAAGTAGAATTAGCAGGTGTATTACATGATTTCTGTAAATATGATGATTTAGGTAAA
    ATGTATCAAATTGTTCGACAATACGAATTAGGTAATGATCTACTGAGTTATGGTAGTGAA
    ATATTGCATGGCCCTGTGTGTGCAGCAATCATGGAACATGAATATGGTATCAATGATGAG
    GAAGTATTAATGGCTATCAAATACCATACTACTGGACGTCAACAAATGACAAAAACTGAA
    AAACTGATTTTTATTGCAGATTACATCGAACCTGGAAGAACAATCCCAGGAGTTGATGAT
    ATTCGAGATATGGCATACAATCAAGGTAGTTTAGATAAGACAATTTATGAAATTTCTAAA
    CGCACAGTACTATTTTTAATACAGAAAGATATTACGGTATATAATAAGACGATTGACTGT
    TTAAATTATTATAACTATAGTGACGAAAGAATAAAGGATGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    585

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS08460 [old locus tag: SAUSA300_1552 ]
  • symbol: SAUSA300_RS08460
  • description: haloacid dehalogenase
  • length: 194
  • theoretical pI: 5.15494
  • theoretical MW: 22463.5
  • GRAVY: -0.49433

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity putative HD superfamily hydrolase (TIGR00488; HMM-score: 140.2)
    and 4 more
    HDIG domain (TIGR00277; HMM-score: 24.5)
    Hypothetical proteins Conserved TIGR00295 family protein (TIGR00295; HMM-score: 18.9)
    HD domain protein, cyanamide hydratase family (TIGR03401; EC 4.2.1.-; HMM-score: 16.3)
    Genetic information processing Transcription Degradation of RNA ribonuclease Y (TIGR03319; EC 3.1.-.-; HMM-score: 11.2)
  • TheSEED: see SAUSA300_1552
  • PFAM:
    HD_PDEase (CL0237) HD; HD domain (PF01966; HMM-score: 44)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005836
    • TAT(Tat/SPI): 0.000227
    • LIPO(Sec/SPII): 0.000722
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVELAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEVLMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISKRTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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