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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS08180 [old locus tag: SAUSA300_1499 ]
- pan locus tag?: SAUPAN004116000
- symbol: SAUSA300_RS08180
- pan gene symbol?: aroK
- synonym:
- product: shikimate kinase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS08180 [old locus tag: SAUSA300_1499 ]
- symbol: SAUSA300_RS08180
- product: shikimate kinase
- replicon: chromosome
- strand: -
- coordinates: 1652864..1653388
- length: 525
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1652864..1653388) NCBI
- BioCyc: SAUSA300_RS08180 BioCyc
- MicrobesOnline: see SAUSA300_1499
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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481ATGAATCATGATAAATCACCAATAATTTTAATTGGTTTCATGGGTACCGGTAAATCTACG
ATTGGTAAATACGTTGCAGATGAGCAAAATTTATCATTTATTGATATAGATTCATATATC
GAAGAGAAGTATAAGTTAACAATACCAGAAATATTTTGTAAACATGGTGAACAATATTTC
AGGAATTTAGAGTTCACATGTTTGCAAGAATGTATTAACACTGCAGATATAATTGCTACT
GGTGGTGGTATTATTGAGAGTGAAGAGGCATTTAATTTTTTGAAAAATCAAAAAAACATT
ATTTGGTTAGATTGTAATATTGATATTATATATAGTCGAATCAATGATGACCCACATCGA
CCTAATGCAAATAATAAGACAATCAAGCAGTTAAATGACTTGTATTGCTCGCGGAATTTA
AGATATAATGAAATCGCATTCAAGAAATTTGATAGTCATTTGCTATCAATTTCAGAAATA
TATTATGAATTGCTAAATTTAATAAAAGCGAGTGATCAGTATTAG60
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525
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS08180 [old locus tag: SAUSA300_1499 ]
- symbol: SAUSA300_RS08180
- description: shikimate kinase
- length: 174
- theoretical pI: 4.87098
- theoretical MW: 20221.8
- GRAVY: -0.344253
⊟Function[edit | edit source]
- reaction: EC 2.7.1.71? ExPASyShikimate kinase ATP + shikimate = ADP + shikimate 3-phosphate
- TIGRFAM: carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 19.5)Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A dephospho-CoA kinase (TIGR00152; EC 2.7.1.24; HMM-score: 19.3)putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 17.6)
- TheSEED: see SAUSA300_1499
- PFAM: P-loop_NTPase (CL0023) SKI; Shikimate kinase (PF01202; HMM-score: 146.2)and 8 moreAAA_18; AAA domain (PF13238; HMM-score: 32.3)AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 20.2)AAA_22; AAA domain (PF13401; HMM-score: 15.6)AAA_33; AAA domain (PF13671; HMM-score: 15.6)Thioredoxin (CL0172) DUF836; Glutaredoxin-like domain (DUF836) (PF05768; HMM-score: 13.5)P-loop_NTPase (CL0023) AAA_24; AAA domain (PF13479; HMM-score: 11.8)CoaE; Dephospho-CoA kinase (PF01121; HMM-score: 11.5)Cytidylate_kin; Cytidylate kinase (PF02224; HMM-score: 11.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Mg2+
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.022874
- TAT(Tat/SPI): 0.000952
- LIPO(Sec/SPII): 0.020455
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446937861 NCBI
- RefSeq: WP_001015117 NCBI
- UniProt: see SAUSA300_1499
⊟Protein sequence[edit | edit source]
- MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKYKLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIWLDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEIYYELLNLIKASDQY
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
SAUSA300_RS04560 NADH dehydrogenase [1] (data from MRSA252) SAUSA300_RS06000 peptide deformylase [1] (data from MRSA252) SAUSA300_RS06220 30S ribosomal protein S2 [1] (data from MRSA252) SAUSA300_RS11380 uracil phosphoribosyltransferase [1] (data from MRSA252) SAUSA300_RS11805 Asp23/Gls24 family envelope stress response protein [1] (data from MRSA252) SAUSA300_RS12065 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_RS12120 30S ribosomal protein S3 [1] (data from MRSA252) SAUSA300_RS13745 pyruvate oxidase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)