PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS06420 [old locus tag: SAUSA300_1188 ]
• pan locus tag^?: SAUPAN003600000
• symbol: SAUSA300_RS06420
• pan gene symbol^?: mutS
• synonym:
• product: DNA mismatch repair protein MutS

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS06420 [old locus tag: SAUSA300_1188 ]
• symbol: SAUSA300_RS06420
• product: DNA mismatch repair protein MutS
• replicon: chromosome
• strand: +
• coordinates: 1306651..1309269
• length: 2619
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1306651..1309269) NCBI
• BioCyc: SAUSA300_RS06420 BioCyc
• MicrobesOnline: see SAUSA300_1188

⊟Phenotype

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⊟DNA sequence

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  ATGTCTAATGTTACACCAATGATGCAGCAATATTTAAAAATAAAATCAGAATACCAAGAT
  TGCTTATTATTTTTTAGACTAGGTGATTTCTATGAAATGTTTTATGAAGATGCCAAGGAG
  GCATCACGTGTACTTGAAATTACTTTAACTAAAAGAGATGCTAAAAAAGAAAATCCAATT
  CCGATGTGTGGTGTTCCGTATCATTCTGCAGATAGTTATATAGATACACTTGTTAATAAT
  GGATATAAAGTAGCTATTTGTGAACAGATGGAAGATCCGAAACAAACGAAAGGTATGGTT
  AGACGTGAGGTAGTAAGAATTGTGACTCCAGGAACTGTGATGGAGCAAGGTGGTGTAGAT
  GATAAACAAAATAACTATATTTTAAGTTTTGTTATGAATCAACCTGAAATTGCGCTTAGT
  TACTGTGATGTTTCTACTGGCGAATTAAAGGTTACACATTTTAATGATGAAGCGACTTTA
  TTAAATGAAATTACGACGATAAACCCTAACGAAGTTGTTATCAATGACAATATTTCCGAT
  AATTTAAAAAGACAAATTAATATGGTGACAGAAACAATAACAGTCAGGGAAACGTTATCA
  TCAGAAATCTATAGTGTGAATCAAACTGAACATAAATTAATGTATCAAGCGACACAATTA
  TTGCTAGATTATATTCATCATACACAAAAACGTGATTTATCGCATATCGAGGATGTTGTT
  CAATATGCAGCTATAGATTATATGAAAATGGATTTTTATGCTAAGAGAAACCTTGAGTTA
  ACGGAAAGCATTCGATTAAAATCAAAAAAAGGAACGCTACTTTGGCTAATGGACGAAACG
  AAAACACCAATGGGAGCACGCCGCTTAAAACAATGGATAGATAGACCACTAATAAGTAAA
  GAACAAATTGAAGCACGATTAGATATCGTTGATGAATTTAGTGCTCATTTCATAGAAAGA
  GACACCTTAAGAACATATCTTAATCAAGTGTATGATATTGAACGTCTTGTTGGGCGTGTT
  AGTTACGGAAATGTTAATGCGAGAGATTTAATTCAACTTAAACATTCCATTTCTGAAATA
  CCGAATATTAAAGCATTACTAAATTCTATGAATCAGAATACTCTTGTACAAGTTAATCAA
  CTAGAACCCCTTGATGATTTACTTGATATATTAGAACAGAGTTTAGTAGAAGAACCACCA
  ATTTCAGTTAAAGATGGCGGACTATTCAAAGTTGGTTTTAATACGCAATTAGATGAATAT
  CTTGAAGCTTCAAAAAACGGAAAAACATGGTTAGCAGAATTACAAGCCAAAGAAAGACAA
  CGTACAGGAATAAAATCATTGAAAATAAGCTTTAATAAAGTGTTTGGTTATTTTATAGAA
  ATAACACGTGCCAACTTGCAAAATTTTGAACCAAGTGAATTTGGTTATATGAGGAAGCAA
  ACGTTATCGAATGCTGAACGTTTTATAACTGATGAACTTAAAGAAAAAGAAGATATCATT
  TTAGGTGCGGAAGACAAAGCCATCGAATTAGAATATCAATTATTTGTTCAGCTACGTGAA
  GAAGTTAAAAAATATACTGAACGTTTACAACAACAAGCTAAAATTATTTCAGAGCTAGAT
  TGTTTACAGAGCTTTGCAGAAATTGCTCAAAAATATAATTACACTAGGCCTTCATTTAGT
  GAAAATAAAACATTAGAATTAGTGGAATCTAGGCACCCAGTAGTGGAAAGAGTAATGGAT
  TATAATGACTATGTGCCTAATAATTGTCGATTAGATAATGAAACATTTATATATTTAATT
  ACAGGTCCGAATATGTCTGGTAAATCGACATATATGAGACAAGTTGCCATAATTAGTATA
  ATGGCCCAAATGGGAGCTTATGTCCCTTGTAAAGAGGCAGTGTTACCTATATTTGATCAA
  ATATTCACTAGAATAGGTGCGGCAGATGATTTGGTTTCAGGTAAGAGTACGTTTATGGTA
  GAAATGCTAGAAGCACAAAAGGCATTAACTTATGCAACAGAGGATAGTTTGATTATTTTC
  GATGAAATTGGACGTGGTACTTCAACGTATGACGGTTTAGCTTTAGCGCAGGCAATGATA
  GAGTATGTAGCTGAAACATCACATGCTAAAACGTTATTTTCAACACATTATCATGAATTG
  ACAACATTAGATCAAGCATTACCAAGTCTAAAAAATGTTCACGTCGCTGCTAATGAATAT
  AAAGGTGAACTTATATTCTTGCATAAAGTCAAAGATGGTGCAGTTGACGATAGTTATGGT
  ATTCAAGTTGCGAAATTAGCTGATTTACCTGAAAAAGTTATTAGCAGAGCACAAGTGATT
  CTAAGCGAGTTTGAAGCGTCTGCTGGTAAAAAATCATCGATATCAAATTTAAAAATGGTC
  GAAAATGAACCTGAAATTAATCAAGAAAATTTAAACTTAAGTGTTGAAGAAACAACTGAT
  ACTTTATCTCAAAAAGACTTTGAACAAGCATCATTTGATTTGTTTGAAAATGATCAAGAA
  AGCGAGATTGAACTACAAATTAAAAATTTGAATTTATCTAATATGACACCAATTGAGGCA
  TTGGTGAAGTTAAGTGAATTACAAAATCAATTAAAATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_RS06420 [old locus tag: SAUSA300_1188 ]
• symbol: SAUSA300_RS06420
• description: DNA mismatch repair protein MutS
• length: 872
• theoretical pI: 4.62947
• theoretical MW: 99903
• GRAVY: -0.376032

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA mismatch repair protein MutS (TIGR01070; HMM-score: 1090.7)
  and 1 more
  Genetic information processing DNA metabolism Other MutS2 family protein (TIGR01069; HMM-score: 147.8)
• TheSEED: see SAUSA300_1188
• PFAM:
  P-loop_NTPase (CL0023) MutS_V; MutS domain V (PF00488; HMM-score: 275.6)
  and 8 more
  no clan defined MutS_I; MutS domain I (PF01624; HMM-score: 164.3)
  MutS_III; MutS domain III (PF05192; HMM-score: 151.2)
  MutS_IV; MutS family domain IV (PF05190; HMM-score: 96.5)
  MutS_II; MutS domain II (PF05188; HMM-score: 65.1)
  P-loop_NTPase (CL0023) AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 17.4)
  AAA_14; AAA domain (PF13173; HMM-score: 16.6)
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 11.3)
  AAA_23; AAA domain (PF13476; HMM-score: 9.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002012
  • TAT(Tat/SPI): 0.000216
  • LIPO(Sec/SPII): 0.000358
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 445995497 NCBI
• RefSeq: WP_000073352 NCBI
• UniProt: see SAUSA300_1188

⊟Protein sequence

• MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRVLEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRREVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATLLNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQATQLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWLMDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIERLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILEQSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKISFNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAIELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLELVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQMGAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDEIGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEYKGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLKMVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMTPIEALVKLSELQNQLK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications