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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS06370 [old locus tag: SAUSA300_1178 ]
  • pan locus tag?: SAUPAN003587000
  • symbol: SAUSA300_RS06370
  • pan gene symbol?: recA
  • synonym:
  • product: DNA recombination/repair protein RecA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS06370 [old locus tag: SAUSA300_1178 ]
  • symbol: SAUSA300_RS06370
  • product: DNA recombination/repair protein RecA
  • replicon: chromosome
  • strand: +
  • coordinates: 1296193..1297236
  • length: 1044
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    TTGGATAACGATCGTCAAAAAGCTTTAGATACAGTAATTAAAAATATGGAGAAATCTTTC
    GGTAAAGGTGCCGTAATGAAGTTGGGTGACAATATAGGTCGCCGAGTTTCAACTACATCA
    ACTGGTTCAGTTACATTAGATAATGCGCTAGGTGTAGGTGGCTATCCTAAAGGACGAATT
    ATTGAAATTTATGGTCCTGAAAGTTCTGGTAAGACAACAGTAGCGCTTCACGCTATTGCT
    GAAGTACAAAGTAATGGCGGGGTGGCAGCATTTATCGATGCTGAACATGCTTTAGATCCA
    GAATATGCTCAAGCATTAGGCGTAGATATCGATAATTTATATTTATCGCAACCGGATCAT
    GGTGAACAAGGTCTTGAAATCGCCGAAGCATTTGTTAGAAGTGGTGCAGTTGATATTGTA
    GTTGTAGACTCAGTTGCTGCTTTAACACCTAAAGCTGAAATTGAAGGAGAAATGGGAGAC
    ACTCACGTTGGTTTACAAGCTCGTTTAATGTCACAAGCGTTACGTAAACTTTCAGGTGCT
    ATTTCTAAATCAAATACAACTGCTATTTTCATCAACCAAATTCGTGAAAAAGTTGGTGTT
    ATGTTCGGTAATCCAGAGACTACACCAGGTGGACGTGCATTAAAATTCTATAGTTCAGTA
    AGACTAGAAGTACGTCGTGCAGAACAGCTTAAACAAGGACAAGAAATTGTAGGTAATAGA
    ACTAAAATTAAAGTCGTTAAAAATAAAGTGGCACCACCATTTAGAGTAGCTGAAGTTGAT
    ATTATGTATGGACAAGGTATTTCTAAAGAGGGTGAACTTATTGATTTAGGTGTTGAAAAC
    GACATCGTTGATAAATCAGGAGCATGGTATTCTTACAATGGCGAACGAATGGGTCAAGGT
    AAGGAAAATGTTAAAATGTACTTGAAAGAAAATCCACAAATTAAAGAAGAAATTGATCGT
    AAATTGAGAGAAAAATTAGGTATATCTGATGGTGATGTTGAAGAAACAGAAGATGCACCA
    AAGTCATTATTTGACGAAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1044

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS06370 [old locus tag: SAUSA300_1178 ]
  • symbol: SAUSA300_RS06370
  • description: DNA recombination/repair protein RecA
  • length: 347
  • theoretical pI: 4.85109
  • theoretical MW: 37656.3
  • GRAVY: -0.398847

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair protein RecA (TIGR02012; HMM-score: 565.3)
    and 11 more
    DNA repair and recombination protein RadB (TIGR02237; HMM-score: 60.3)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair and recombination protein RadA (TIGR02236; HMM-score: 41.2)
    meiotic recombinase Dmc1 (TIGR02238; HMM-score: 36.9)
    DNA repair protein RAD51 (TIGR02239; HMM-score: 31.4)
    KaiC domain protein, PAE1156 family (TIGR03881; HMM-score: 20.6)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair protein RadA (TIGR00416; HMM-score: 16.5)
    KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 16.2)
    2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 14)
    Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04520; EC 3.6.3.-; HMM-score: 12.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase (TIGR00521; EC 4.1.1.36,6.3.2.5; HMM-score: 11.4)
    putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 10.5)
  • TheSEED: see SAUSA300_1178
  • PFAM:
    P-loop_NTPase (CL0023) RecA; recA bacterial DNA recombination protein (PF00154; HMM-score: 473.1)
    and 10 more
    Rad51; Rad51 (PF08423; HMM-score: 44.4)
    ATPase; KaiC (PF06745; HMM-score: 36.4)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 20.3)
    AAA_24; AAA domain (PF13479; HMM-score: 16.3)
    AAA_25; AAA domain (PF13481; HMM-score: 16.2)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 15.7)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 13.4)
    H-int (CL0363) PT-HINT; Pretoxin HINT domain (PF07591; HMM-score: 13.2)
    P-loop_NTPase (CL0023) CobU; Cobinamide kinase / cobinamide phosphate guanyltransferase (PF02283; HMM-score: 13)
    NADP_Rossmann (CL0063) DFP; DNA / pantothenate metabolism flavoprotein (PF04127; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.048833
    • TAT(Tat/SPI): 0.065314
    • LIPO(Sec/SPII): 0.00261
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSVTLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEYAQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGDTHVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYSSVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDLGVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVEETEDAPKSLFDEE

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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