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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS06305 [old locus tag: SAUSA300_1165 ]
  • pan locus tag?: SAUPAN003573000
  • symbol: SAUSA300_RS06305
  • pan gene symbol?: ribC
  • synonym:
  • product: bifunctional riboflavin kinase/FMN adenylyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS06305 [old locus tag: SAUSA300_1165 ]
  • symbol: SAUSA300_RS06305
  • product: bifunctional riboflavin kinase/FMN adenylyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1280336..1281307
  • length: 972
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAGTCATAGAAGTGACACATCCTATACAATCTAAACAGTATATTACAGAGGATGTT
    GCAATGGCATTCGGATTTTTCGATGGCATGCATAAAGGTCATGACAAAGTCTTTGATATA
    TTAAACGAAATAGCTGAGGCACGCAGTTTAAAAAAAGCGGTGATGACATTTGATCCGCAT
    CCGTCTGTCGTGTTGAATCCTAAAAGAAAACGAACAACGTATTTAACGCCACTTTCAGAT
    AAAATCGAAAAAATTAGCCAACATGATATTGATTATTGTATAGTGGTTAATTTTTCATCT
    AGGTTTGCTAATGTGAGCGTAGAAGATTTTGTTGAAAATTATATAATTAAAAATAATGTA
    AAAGAAGTCATTGCTGGTTTTGATTTTACTTTTGGTAAATTTGGAAAAGGTAATATGACT
    GTACTTCAAGAATATGATGCGTTTAATACGACAATTGTGAGTAAACAAGAAATTGAAAAT
    GAAAAAATTTCTACAACTTCTATTCGTCAAGATTTAATCAATGGTGAGTTGCAAAAAGCG
    AATGATGCTTTAGGCTATATATATTCTATTAAAGGCACTGTAGTGCAAGGTGAAAAAAGG
    GGAAGAACTATTGGCTTCCCAACAGCTAACATTCAACCTAGTGATGATTATTTGTTACCT
    CGTAAAGGTGTTTATGCTGTTAGTATTGAAATCGGCACTGAAAATAAATTATATCGAGGG
    GTAGCTAACATAGGTGTAAAGCCAACATTTCATGATCCTAACAAAGCAGAAGTTGTCATC
    GAAGTGAATATCTTTGACTTTGAGGATAATATTTATGGTGAACGAGTGACCGTGAATTGG
    CATCATTTCTTACGTCCTGAGATTAAATTTGATGGTATCGACCCATTAGTTAAACAAATG
    AACGATGATAAATCGCGTGCTAAATATTTATTAGCAGTTGATTTTGGTGATGAAGTAGCT
    TATAATATCTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    972

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS06305 [old locus tag: SAUSA300_1165 ]
  • symbol: SAUSA300_RS06305
  • description: bifunctional riboflavin kinase/FMN adenylyltransferase
  • length: 323
  • theoretical pI: 5.89394
  • theoretical MW: 36691.4
  • GRAVY: -0.314861

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.2?  ExPASy
    FAD synthetase ATP + FMN = diphosphate + FAD
    EC 2.7.1.26?  ExPASy
    Riboflavin kinase ATP + riboflavin = ADP + FMN
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD riboflavin biosynthesis protein RibF (TIGR00083; EC 2.7.1.26,2.7.7.2; HMM-score: 286.3)
    and 1 more
    cytidyltransferase-like domain (TIGR00125; HMM-score: 15.7)
  • TheSEED: see SAUSA300_1165
  • PFAM:
    HUP (CL0039) FAD_syn; FAD synthetase (PF06574; HMM-score: 164.4)
    and 2 more
    no clan defined Flavokinase; Riboflavin kinase (PF01687; HMM-score: 124.5)
    HUP (CL0039) CTP_transf_like; Cytidylyltransferase-like (PF01467; HMM-score: 15.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004761
    • TAT(Tat/SPI): 0.000202
    • LIPO(Sec/SPII): 0.0003
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEIAEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRFANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIENEKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYLLPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERVTVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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