PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS05175 [old locus tag: SAUSA300_0963 ]
• pan locus tag^?: SAUPAN003270000
• symbol: SAUSA300_RS05175
• pan gene symbol^?: qoxA
• synonym:
• product: quinol oxidase subunit 2

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS05175 [old locus tag: SAUSA300_0963 ]
• symbol: SAUSA300_RS05175
• product: quinol oxidase subunit 2
• replicon: chromosome
• strand: -
• coordinates: 1055592..1056692
• length: 1101
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1055592..1056692) NCBI
• BioCyc: SAUSA300_RS05175 BioCyc
• MicrobesOnline: see SAUSA300_0963

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  
  GTGTCAAAATTTAAGTCTTTGCTTCTATTATTTGGCACACTAATTTTACTTAGTGGTTGT
  TCAAATATAGAAATTTTCAACGCAAAAGGGCCAGTAGCAAGTAGTCAGAAGTTCTTGATT
  CTTTATTCAATCGTCTTCATGCTTGTTATTTGTTTCGTTGTACTTGGCATGTTCGCCATT
  TTTATTTACAAGTATAGTTATAATAAGAATGCCGAATCTGGTAAGATGCACCATAATGCC
  ATCATTGAAACAATATGGTTTGTAATACCTATCATAATCGTTGCTGCTTTAGCTATTCCT
  ACAGTTAAAACTTTATACGATTACGAAAAACCACCGAAGAGTGAGAAAGATCCAATGGTT
  GTATATGCAGTAAGTGCAGGATACAAATGGTTCTTTGCTTATCCAGATGAACATATAGAG
  ACTGTTAATACATTAACAATCCCTAAAGATCGTCCTGTTGTATTTAAGCTTCAAGCTATG
  GATACAATGACAAGTTTCTGGATTCCACAATTAGGTGGTCAAAAATATGCCATGACTGGC
  ATGACAATGAATTGGACGTTAGAAGCATCTCAAACTGGTACGTTCAGAGGTCGTAACTCT
  AACTTCAATGGTGAAGGATTCTCACGTCAAACATTTAAAGTAAATGCCGTTAGTCAAAAA
  GACTACGACAAATGGGTGAAAGAAGTTAAAGGTAAGAAAACGTTAGATCAAGATACATTT
  GATAAACAATTATTACCAAGCACACCTAATAAAGCTTTAGAATTTAATGGTACACATATG
  GCGTTTGTTGATCCAGCAGCCGATCCAGAATACATCTTCTATGCATATAAACGTTTCAAT
  TTCGAATTAAAAGATCCAAACTTCACTAGTGAAGAAAATATGTTTAAAGATGTATCAGAC
  AAACCATTAATACCTGCTCGTAAAGCTCAAATTACAAATGCGAACTATAAACGACATGGT
  ATGAAGTTGATGATTCTTGGAAATGACGAACCATATAACAATGAGTTCAAGAAAGATGAA
  TCGAAAAATGCGAAAGAAATGAAGAAAATTTCAAAAGATGCGCAAGATCAAGACAATGAT
  GATCATGGAGGTGGACATTAA
  60
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  1101
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS05175 [old locus tag: SAUSA300_0963 ]
• symbol: SAUSA300_RS05175
• description: quinol oxidase subunit 2
• length: 366
• theoretical pI: 9.00291
• theoretical MW: 41776.8
• GRAVY: -0.337158

⊟Function

• reaction:
  EC 1.9.3.-?  ExPASy
  EC 1.10.3.-?  ExPASy
  2 a quinol + O₂ = 2 a quinone + 2 H₂O?
• TIGRFAM:
  Metabolism Energy metabolism Electron transport cytochrome aa3 quinol oxidase, subunit II (TIGR01432; EC 1.10.3.-; HMM-score: 430.2)
  and 2 more
  Metabolism Energy metabolism Electron transport ubiquinol oxidase, subunit II (TIGR01433; EC 1.10.3.-; HMM-score: 181.3)
  Metabolism Energy metabolism Electron transport cytochrome c oxidase, subunit II (TIGR02866; EC 1.9.3.1; HMM-score: 135.6)
• TheSEED: see SAUSA300_0963
• PFAM:
  no clan defined COX2_TM; Cytochrome C oxidase subunit II, transmembrane domain (PF02790; HMM-score: 45.7)
  and 8 more
  CU_oxidase (CL0026) COX2; Cytochrome C oxidase subunit II, periplasmic domain (PF00116; HMM-score: 23.6)
  no clan defined Vps55; Vacuolar protein sorting 55 (PF04133; HMM-score: 15.1)
  DUF6796; Family of unknown function (DUF6796) (PF20599; HMM-score: 15.1)
  MpPF26; M penetrans paralogue family 26 (PF07666; HMM-score: 13.8)
  DUF6688; DUF6688 N-terminal domain (PF20394; HMM-score: 13.5)
  Ninjurin; Ninjurin (PF04923; HMM-score: 11.6)
  Bac_export_2; FlhB HrpN YscU SpaS Family (PF01312; HMM-score: 10.9)
  DHHC; DHHC palmitoyltransferase (PF01529; HMM-score: 8.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 3
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9975
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0025
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
  • SP(Sec/SPI): 0.000508
  • TAT(Tat/SPI): 0.000057
  • LIPO(Sec/SPII): 0.998769
  • Cleavage Site: CS pos: 19-20. LSG-CS. Pr: 0.9995
• predicted transmembrane helices (TMHMM): 3

⊟Accession numbers

• GI: 445954981 NCBI
• RefSeq: WP_000032836 NCBI
• UniProt: see SAUSA300_0963

⊟Protein sequence

• MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSIVFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTVKTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAMDTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVSQKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAYKRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNNEFKKDESKNAKEMKKISKDAQDQDNDDHGGGH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for JSNZ, NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications