From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS04615 [old locus tag: SAUSA300_0855 ]
  • pan locus tag?: SAUPAN003053000
  • symbol: SAUSA300_RS04615
  • pan gene symbol?: mnhA1
  • synonym:
  • product: Na+/H+ antiporter subunit A

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS04615 [old locus tag: SAUSA300_0855 ]
  • symbol: SAUSA300_RS04615
  • product: Na+/H+ antiporter subunit A
  • replicon: chromosome
  • strand: -
  • coordinates: 931689..934094
  • length: 2406
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    GTGAGTTTGTTACATATTGCGGTGATATTACCGTTAATATTCGCACTCATTATTCCTATC
    CTGTATAGATTTTTTAAACGTATACATTTAGGATGGTTCGTATTACCTGTCCCTATCGTC
    ATATTTATTTATATGCTTACATTAATTAAAACGACGATGTCAGGTAATACTGTAATGAAA
    ACCTTAAATTGGATGCCTCATTTTGGTATGAACTTTGATTTATATCTTGATGGCTTAGGT
    TTACTCTTTAGTTTGCTTATTTCAGGAATCGGCAGTTTAGTAGTATTATATTCAATCGGC
    TACTTAAGTAAGTCAGAACAACTAGGGAATTTTTATTGTTATTTATTACTATTTATGGGT
    GCAATGTTAGGTGTCGTACTTTCAGATAATGTTATTATTCTATATTTATTCTGGGAGCTA
    ACGTCATTTTCTAGTTTTTTACTTATTTCATTCTGGCGCGAACGACAAGCATCTATATAT
    GGTGCTCAAAAATCGCTCATAATCACAGTATTTGGTGGTCTATCATTATTAGGTGGTATC
    ATCTTACTCGCTATACCAACACAATCATTTAGCATTCAGTACATGATTCAACATGCTTCT
    GAAATTCAAAATAGCCCATTCTTCATTTTTGCGATGATTTTAATCATGATTGGTGCTTTT
    ACAAAATCTGCTCAGTTTCCATTTTATATTTGGCTACCAGATGCAATGGAAGCACCTACA
    CCAGTCAGTGCTTATCTTCATTCAGCAACAATGGTTAAAGCAGGGTTATATTTAATCGCT
    CGAATGACACCAATCTTCGCAGCATCGCAAGGTTGGGTATGGACTGTCACACTCGTTGGT
    TTAATTACATTATTCTGGGCTTCGCTAAACGCAACAAAACAACAAGACTTGAAAGGTATA
    CTGGCATTTTCAACAGTATCCCAATTAGGTATGATTATGGCGATGCTTGGAATCGGTGCC
    ATTAGTTATCACTATCAAGGCGATGATAGCAAAATATATGCTGCTGCATTTACGGCTGCA
    ATATTCCATTTAATCAACCATGCAACATTTAAAGGTGCCCTATTTATGATTACAGGTGCA
    GTGGATCATTCTACTGGTACACGTGATGTTAAGAAATTAGGTGGCTTACTAACAATTATG
    CCTATTTCATTTACAATCACTGTTATTACAGCATTAAGTATGGCTGGTGTACCACCGTTC
    AATGGTTTCTTATCAAAAGAATCATTTTTAGAAACAACATTTACAGCAAGCCAAGCAAAT
    TTATTCAGTGTTGATACATTAGGCTACCTATTCCCAATCATTGGTATAGTGGGCAGTGTC
    TTCACATTTGTATATTCAATTAAATTTATAATGCATATATTTTTCGGACAATATAAACCT
    GAACAATTACCTAAAAAAGCACACGAAGTATCAATATTAATGCTTTTATCACCAGCTATT
    TTAGCTACATTAGTAATTGTATTTGGTTTATTCCCTGGCATTTTAACTAATTCGATTATT
    GAACCAGCTACATCTTCGATTAATCATACGGTAATTGATGATGTTGAATTCCATATGTTC
    CATGGATTAACGCCTGCCTTTTTATCAACATTAGTGATTTATATTTTAGGTATCTTACTA
    ATTGTGACATTTAGTTATTGGGTGAAATTGTTGCAACGACAACCTGGTAAACTAACATTC
    AATTATTGGTACAACAGAAGTGCAAATGTTATTCCAAACTACTCTGAAAAGATGACGAAT
    AGCTATGTTACCGATTATTCACGTAATAATTTAGTTATCATTTTTGGTGCATTAATTTTA
    TTAACATTTGTCACTATATTCTCAGTACCTTTCAACATTAATTTTAAAGACGTTAGTCCG
    ATTAGAATTTTCGAAGTTTGTATCGTAATTTTATTATTAAGTGCTGCCTTTTTAATTCTT
    TTCGCTAAATCAAGATTATTTAGCATCATTATGTTAAGTGCAGTTGGATATGCTGTGTCT
    GTATTGTTTATATTCTTTAAAGCACCCGACTTAGCATTGACACAGTTTGTCGTTGAATCT
    ATTTCAACAGCATTGTTCTTATTATGCTTCTACCACTTACCAAATTTAAATCGTTATAAT
    GAAAAGCGCTCATTCCAGCTTACTAATGCTTTAATTGCTGGTGGTGTTGGTTTGTCCGTC
    ATTATTATCGGTTTAATTGCATACGGAAATAGACATTTTGAATCGATATCTAAGTTTTAT
    CAAGAGCACGTTTATGATTTAGCTCACGGTAAAAACATGGTAAACGTCATACTTGTTGAT
    TTCCGTGGTATGGATACTTTATTCGAGTCATCTGTGCTTGGTATCGCAGGATTAGCTGTA
    TATACAATGATTAAATTACGTAAAAAGCGTCAAACGCAAGGTAACGAGGTGAAAAATCAT
    GAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2406

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS04615 [old locus tag: SAUSA300_0855 ]
  • symbol: SAUSA300_RS04615
  • description: Na+/H+ antiporter subunit A
  • length: 801
  • theoretical pI: 9.38625
  • theoretical MW: 89395.5
  • GRAVY: 0.760549

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00940; HMM-score: 1436)
    and 6 more
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 332.8)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 203.8)
    NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 157.6)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 129.7)
    Metabolism Transport and binding proteins Cations and iron carrying compounds multicomponent K+:H+antiporter (TIGR00944; HMM-score: 113)
    mesaconyl-CoA isomerase (TIGR04253; EC 5.-.-.-; HMM-score: 11.1)
  • TheSEED: see SAUSA300_0855
  • PFAM:
    ComplexI-N (CL0425) Proton_antipo_M; NADH:quinone oxidoreductase/Mrp antiporter, TM (PF00361; HMM-score: 208.5)
    and 5 more
    no clan defined MbhD; MBH, subunit D (PF13244; HMM-score: 73.6)
    MbhE; MBH, subunit E (PF20501; HMM-score: 60.5)
    Proton_antipo_N; NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus (PF00662; HMM-score: 38)
    DUF2106; Predicted membrane protein (DUF2106) (PF09879; HMM-score: 11.8)
    MNSV_P7B; Melon necrotic spot virus P7B protein (PF06692; HMM-score: 7.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 21
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 1
    • Cell wall & surface Score: 0
    • Extracellular Score: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001449
    • TAT(Tat/SPI): 0.000287
    • LIPO(Sec/SPII): 0.002918
  • predicted transmembrane helices (TMHMM): 21

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIYMLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYLSKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIYGAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIGAFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTVTLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYAAAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITALSMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFIMHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSINHTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNRSANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIFEVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESISTALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFYQEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVKNHE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for JSNZ

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_RS04615&oldid=93329"