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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS02875 [old locus tag: SAUSA300_0538 ]
- pan locus tag?: SAUPAN002325000
- symbol: SAUSA300_RS02875
- pan gene symbol?: —
- synonym:
- product: UDP-glucose 4-epimerase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS02875 [old locus tag: SAUSA300_0538 ]
- symbol: SAUSA300_RS02875
- product: UDP-glucose 4-epimerase
- replicon: chromosome
- strand: +
- coordinates: 603879..604844
- length: 966
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (603879..604844) NCBI
- BioCyc: SAUSA300_RS02875 BioCyc
- MicrobesOnline: see SAUSA300_0538
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGAAAAAAATTATGATTACTGGTGCATTAGGACAAATTGGTACAGAATTAGTTGTTAAG
TGCAGAGAAATTTATGGGACAGATAATGTTCTTGCTACAGATATTAGGGAACCTGAAGCA
GACTCACCTGTACAAAATGGACCATTTGAAATCTTAGACGTAACAGATCGTGACCGTATG
TTTGAGTTAGTTAGGGACTTTGAAGCGGATAGTCTAATGCATATGGCAGCATTATTATCA
GCAACTGCTGAGAAAAATCCAATTCTAGCTTGGGATTTAAATATGGGTGGATTAATGAAT
GCATTAGAAGCTGCAAGAACTTATAATTTGCACTTTTTCACACCAAGTTCAATTGGTGCA
TTTGGAGACTCAACTCCTAAAGTTAATACGCCACAAGTAACGATTCAGCAACCTACGACA
ATGTATGGTGTAAATAAAGTAGCTGGAGAATTATTGTGTCAATACTATTTCAAACGTTTT
GGTGTAGATACAAGAAGTGTTAGATTCCCAGGTTTAATCTCGCATGTTAAAGAGCCAGGT
GGCGGTACTACAGACTATGCTGTTGAAATATACTTCAAAGCAGTAAGAGAGGGTCATTAT
ACAAGCTTCATAGATAAAGGCACGTATATGGATATGATGTATATGGATGATGCAATTGAA
GCAATTATTAAACTTATGGAAGCAGACGACGCTAAATTAGAAACTAGAAATGGTTATAAT
TTGAGCGCAATGAGTTTTGATCCAGAGATGGTAAAAGAAGCAATTCAAGAATACTATCCC
AATTTTACATTAGATTACGATGTTGATCCTATTAGACAAGGTATCGCTAATAGTTGGCCG
GATTCTATTGATACAAGCTGTTCACGTGGCGAATGGGGATTTGATCCTAAATATGATTTA
GCGAGCATGACTAAATTAATGTTAGAAGCTATTGAACAAAAAGATACTGTTAAAAATAAT
AACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS02875 [old locus tag: SAUSA300_0538 ]
- symbol: SAUSA300_RS02875
- description: UDP-glucose 4-epimerase
- length: 321
- theoretical pI: 4.35464
- theoretical MW: 36052.7
- GRAVY: -0.311526
⊟Function[edit | edit source]
- reaction: EC 5.1.3.2? ExPASyUDP-glucose 4-epimerase UDP-alpha-D-glucose = UDP-alpha-D-galactose
- TIGRFAM: Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides ADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 44.8)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-4-dehydrorhamnose reductase (TIGR01214; EC 1.1.1.133; HMM-score: 39.5)and 5 moreEnergy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 30.9)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 30.1)NAD dependent epimerase/dehydratase, LLPSF_EDH_00030 family (TIGR04180; HMM-score: 21.6)UDP-N-acetylglucosamine 4,6-dehydratase (inverting) (TIGR03589; EC 4.2.1.115; HMM-score: 21.5)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides CDP-glucose 4,6-dehydratase (TIGR02622; EC 4.2.1.45; HMM-score: 20)
- TheSEED: see SAUSA300_0538
- PFAM: NADP_Rossmann (CL0063) Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 88.8)and 5 moreGDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 56.3)RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 43.7)Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 35.8)3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 22.3)NAD_binding_4; Male sterility protein (PF07993; HMM-score: 16.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.08838
- TAT(Tat/SPI): 0.000732
- LIPO(Sec/SPII): 0.188772
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446645955 NCBI
- RefSeq: WP_000723301 NCBI
- UniProt: see SAUSA300_0538
⊟Protein sequence[edit | edit source]
- MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPVQNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNALEAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRFGVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDAIEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIANSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
SAUSA300_RS05360 dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex [1] (data from MRSA252) SAUSA300_RS06220 30S ribosomal protein S2 [1] (data from MRSA252) SAUSA300_RS08975 pyruvate kinase [1] (data from MRSA252) SAUSA300_RS12065 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_RS12120 30S ribosomal protein S3 [1] (data from MRSA252) SAUSA300_RS14295 arginine deiminase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CcpA see SAUSA300_0538
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)