PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS00320 [old locus tag: SAUSA300_0063 ]
• pan locus tag^?: SAUPAN000812000
• symbol: SAUSA300_RS00320
• pan gene symbol^?: —
• synonym:
• product: Crp/Fnr family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS00320 [old locus tag: SAUSA300_0063 ]
• symbol: SAUSA300_RS00320
• product: Crp/Fnr family transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 70875..71564
• length: 690
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (70875..71564) NCBI
• BioCyc: SAUSA300_RS00320 BioCyc
• MicrobesOnline: see SAUSA300_0063

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTATGAAGAAAATATTTATATTAAAAATTCAGAATATGAATTTGATAATAATCTTAAA
  CAATTAGCATCATACTTAAATATTCCTGTTAGTATTGTTAGACCTTATAAAGAGGATTTA
  ACACTTTATCAATATAAAAAAGGACAAGTCATATATCATTCAACTGATCAAATAAAATTT
  GTATACTTTTTAGTAAATGGTTGTATTTTACATGAATCTTCTAATATTACTGGTGACAAT
  TATTTAAGATTAAGTAAAGACGAAAATATATTTCCAATGAACTTCATATTTAATGAAACC
  CCTGCACCATATGAAATATGTACAGCTTTGACAGATTGTAAAATATTAACTTTACCGAAA
  GATTTACTTGAGTATTTATGTAGAAAGCATAATGAAATATTTGAAAGTCTCTTCAAGAAA
  CTTAATGAGACTATTCAATTTCAAGTAGAATATATTATGGCGTTAAGAGCTAATTCAGCT
  AAAGAAAGAATTGAAAGAATACTACAAATTTTATGCCTTTCAATTGGGGATGATAATGGA
  GAATTCTATGAATTAAAACAAATTATGACTGTTCAATTAATAAGTAATTTATCTGGACTT
  AACAGAAAAACTACTGGTGAAATAATCAGAGAATTAAAAATAGAAAATATTATATATCAA
  GATAAAAGAAATTGGATTATAAAAAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS00320 [old locus tag: SAUSA300_0063 ]
• symbol: SAUSA300_RS00320
• description: Crp/Fnr family transcriptional regulator
• length: 229
• theoretical pI: 5.81356
• theoretical MW: 27098.1
• GRAVY: -0.310917

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions global nitrogen regulator NtcA (TIGR03697; HMM-score: 26.9)
• TheSEED: see SAUSA300_0063
• PFAM:
  Cupin (CL0029) cNMP_binding; Cyclic nucleotide-binding domain (PF00027; HMM-score: 34.7)
  and 2 more
  HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 19.7)
  no clan defined DUF3991; Protein of unknown function (DUF3991) (PF13154; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010074
  • TAT(Tat/SPI): 0.000304
  • LIPO(Sec/SPII): 0.002068
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446194926 NCBI
• RefSeq: WP_000272781 NCBI
• UniProt: see SAUSA300_0063

⊟Protein sequence

• MYEENIYIKNSEYEFDNNLKQLASYLNIPVSIVRPYKEDLTLYQYKKGQVIYHSTDQIKFVYFLVNGCILHESSNITGDNYLRLSKDENIFPMNFIFNETPAPYEICTALTDCKILTLPKDLLEYLCRKHNEIFESLFKKLNETIQFQVEYIMALRANSAKERIERILQILCLSIGDDNGEFYELKQIMTVQLISNLSGLNRKTTGEIIRELKIENIIYQDKRNWIIKK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications