PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1978 [new locus tag: SAUSA300_RS10865 ]
• pan locus tag^?: SAUPAN005203000
• symbol: SAUSA300_1978
• pan gene symbol^?: fhuD1
• synonym:
• product: ferric hydroxamate receptor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1978 [new locus tag: SAUSA300_RS10865 ]
• symbol: SAUSA300_1978
• product: ferric hydroxamate receptor
• replicon: chromosome
• strand: -
• coordinates: 2133798..2134709
• length: 912
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913731 NCBI
• RefSeq: YP_494629 NCBI
• BioCyc: see SAUSA300_RS10865
• MicrobesOnline: 1293493 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAAAAGATTAATTGGAATTTTATTATGTAATTTATTTATTTTGACTGCGTGTTCAGCA
  TCTGTCGATAAAACAAGTAACTCGACAAAGACTACAGACTATAAAATTGAAAATGGTGAA
  ACACTGAAAGTACCAGAGAAACCTAAAAGAGTTGCTGTATTAACTGGATTTTATGTTGGT
  GATTTTATAAAGTTAGGAATCAAACCAATTGCTGTTTCAGATATAACTAAAGATTCTTCA
  ATTTTAAAACCTTATTTAAAAGGGGTTGATTATATTGGAGAAAATGATGTTGAAAGAGTT
  GCTAAAGCAAAACCAGATTTAATTGTTGTAGATGCTATGGATAAAAATATAAAAAAATAC
  CAAAAAATAGCGCCAACAATCCCATATACATACAATAAATACAATCATAAAGAAATATTA
  AAAGAAATAGGCAAGTTGACTAATAATGAAGATAAAGCTAAAAAATGGATTGAAGAGTGG
  GACGATAAAACTAGAAAAGATAAAAAAGAAATTCAAAGTAAAATTGGTCAAGCAACAGCA
  TCTGTGTTTGAACCAGATGAAAAGCAAATATACATATATAACTCTACATGGGGTCGTGGT
  TTGGATATTGTTCATGATGCATTCGGTATGCCAATGACAAAGCAATATAAAGATAAATTA
  CAAGAAGATAAAAAAGGCTATGCTTCCATTTCAAAAGAAAATATTAGTAAATATGCTGGT
  GATTATATATTTTTAAGTAAACCTTCGTACGGAAAATTTGATTTTGAAAAAACACATACA
  TGGCAGAATATTGAAGCTGTAAAAAAAGGACATGTAATTTCATATAAAGCAGAAGATTAT
  TGGTTCACAGATCCTATTACATTAGAACATTTGAGAAGTAAATTAAAAAAAGAAATTTTA
  AATAAAAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1978 [new locus tag: SAUSA300_RS10865 ]
• symbol: SAUSA300_1978
• description: ferric hydroxamate receptor
• length: 303
• theoretical pI: 9.74449
• theoretical MW: 34842.9
• GRAVY: -0.646865

⊟Function

• TIGRFAM:
  heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 26.4)
  and 1 more
  putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 15.6)
• TheSEED  :
  • Ferrichrome-binding periplasmic protein precursor (TC 3.A.1.14.3)
• PFAM:
  Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 174.4)
  and 2 more
  no clan defined RXLR; RXLR phytopathogen effector protein, Avirulence activity (PF16810; HMM-score: 15.1)
  NADH_dhqG_C; NADH-ubiquinone oxidoreductase subunit G, C-terminal (PF09326; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.81
  • Cellwall Score: 0.09
  • Extracellular Score: 0.1
  • Internal Helix: 1
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: -0.33
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: FILTACS
• SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
  • SP(Sec/SPI): 0.000952
  • TAT(Tat/SPI): 0.000088
  • LIPO(Sec/SPII): 0.998672
  • Cleavage Site: CS pos: 17-18. LTA-CS. Pr: 0.9992
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161142 NCBI
• RefSeq: YP_494629 NCBI
• UniProt: A0A0H2XGM1 UniProt

⊟Protein sequence

• MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKVPEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAKAKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEWDDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKDKLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYKAEDYWFTDPITLEHLRSKLKKEILNKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications