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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1922 [new locus tag: SAUSA300_RS10540 ]
- pan locus tag?: SAUPAN005023000
- symbol: sak
- pan gene symbol?: sak
- synonym:
- product: staphylokinase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_1922 [new locus tag: SAUSA300_RS10540 ]
- symbol: sak
- product: staphylokinase
- replicon: chromosome
- strand: -
- coordinates: 2088425..2088916
- length: 492
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3913751 NCBI
- RefSeq: YP_494573 NCBI
- BioCyc: see SAUSA300_RS10540
- MicrobesOnline: 1293437 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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481ATGCTCAAAAGAGGTTTATTATTTTTAACTGTTTTATTGTTATTATTCTCATTTTCTTCA
ATTACTAATGAGGTAAGTGCATCAAGTTCATTCGACAAAGGAAAATATAAAAAAGGCGAT
GACGCGAGTTATTTTGAACCAACAGGCCCGTATTTGATGGTAAATGTGACTGGAGTTGAT
GGTAAAGGAAATGAATTGCTATCCCCTCATTATGTCGAGTTTCCTATTAAACCTGGGACT
ACACTTACAAAAGAAAAAATTGAATACTATGTCGAATGGGCATTAGATGCGACAGCATAT
AAAGAGTTTAGAGTAGTTGAATTAGATCCAAGCGCAAAGATCGAAGTCACTTATTATGAT
AAGAATAAGAAAAAAGAAGAAACGAAGTCTTTCCCTATAACAGAAAAAGGTTTTGTTGTC
CCAGATTTATCAGAGCATATTAAAAACCCTGGATTCAACTTAATTACAAAGGTTATTATA
GAAAAGAAATAA60
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492
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1922 [new locus tag: SAUSA300_RS10540 ]
- symbol: Sak
- description: staphylokinase
- length: 163
- theoretical pI: 7.63429
- theoretical MW: 18474.1
- GRAVY: -0.302454
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.02
- Extracellular Score: 9.98
- Internal Helix: 1
- LocateP: Secretory(released) (with CS)
- Prediction by SwissProt Classification: Extracellular
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: NEVSASSS
- SignalP: Signal peptide SP(Sec/SPI) length 27 aa
- SP(Sec/SPI): 0.976782
- TAT(Tat/SPI): 0.001652
- LIPO(Sec/SPII): 0.019831
- Cleavage Site: CS pos: 27-28. VSA-SS. Pr: 0.8969
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASYFEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKEFRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVIIEKK
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: SaeR (activation) regulon
SaeR (TF) important in Virulence; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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