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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1494 [new locus tag: SAUSA300_RS08155 ]
  • pan locus tag?: SAUPAN004110000
  • symbol: SAUSA300_1494
  • pan gene symbol?: lipM
  • synonym:
  • product: hypothetical protein

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1494 [new locus tag: SAUSA300_RS08155 ]
  • symbol: SAUSA300_1494
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1647044..1647874
  • length: 831
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACTGAAACTTGGAATTTTATTAATACAGGAAGTAAAGACCCATATTATAATATGGCA
    ATGGATGAAGCGTTATTAAATTTTGTCTCTAGAGGTGAAATTGATCCAGTTATACGTTTT
    TACACATGGAATCCTGCAACATTATCAATAGGGTATTTCCAAAGGCTACAAAAAGAAATT
    GATATTGATAAGGTTAAAGAGAAAGGTTTTGGTCTCGTAAGGCGTCAAACTGGTGGCCGT
    GGGGTGCTACATGATAAAGAGTTAACTTACAGTGTGATTGTGCCTGAATCGCATCCGAAT
    ATGCCTTCTACAGTTACAGAAGCGTACAGAGTGATTTCACAAGGATTATTAGAAGGATTT
    AAAAACTTAGGATTTGATACTTATTTTGCGGTACCTAAAACGCCTGAAGAGCGTCAAAAA
    TTAAAACAACCTCGAAGTTCAGTGTGTTTTGATGCACCAAGTTGGTATGAGCTAGTTGTT
    GAAGGCCGTAAGATTGCTGGGAGTGCTCAAACGAGACAAAAGGGCGTAATCCTCCAACAC
    GGTTCGATTTTACAAGATATTGATATTGATGAACTGTTTGATATGTTTATTTATAAAAAT
    GAAAGATTAAAATTAAAAATGAAAGAGGCATTCGTTGAAAAAGCAGTTGCCATCAATGAT
    ATTTCAGATGAACACATTACTATTAGTCAAATGGAAGAAGCTTTCGAGAAAGGTTTTAAG
    AAAGGTTTAAATATAGAATTTAAGCCACTTGAATTAACAGAAGCTCAATTGGCAGAAGTG
    GAAGAATTAACTGAAAAATATCGTTCAGATGAATGGATGTTTAGAAAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    831


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1494 [new locus tag: SAUSA300_RS08155 ]
  • symbol: SAUSA300_1494
  • description: hypothetical protein
  • length: 276
  • theoretical pI: 5.15375
  • theoretical MW: 31915.2
  • GRAVY: -0.477536

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.181?  ExPASy
    Lipoyl(octanoyl) transferase Octanoyl-[acyl-carrier-protein] + protein = protein N6-(octanoyl)lysine + [acyl-carrier-protein]
  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair lipoyltransferase and lipoate-protein ligase (TIGR00545; HMM-score: 100.5)
  • TheSEED  :
    • Octanoate-[acyl-carrier-protein]-protein-N-octanoyltransferase, LipM
    Carbohydrates Central carbohydrate metabolism Dehydrogenase complexes  Lipoate-protein ligase A
    and 1 more
    Cofactors, Vitamins, Prosthetic Groups, Pigments Lipoic acid Lipoic acid metabolism  Lipoate-protein ligase A
  • PFAM:
    tRNA_synt_II (CL0040) LplA-B_cat; Lipoyl protein ligase A/B catalytic domain (PF21948; HMM-score: 153.4)
    and 1 more
    BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 93.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9806
    • Cytoplasmic Membrane Score: 0.0083
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.011
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002278
    • TAT(Tat/SPI): 0.000151
    • LIPO(Sec/SPII): 0.000409
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWNPATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMPSTVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVVEGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAINDISDEHITISQMEEAFEKGFKKGLNIEFKPLELTEAQLAEVEELTEKYRSDEWMFRK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]


⊟Relevant publications[edit | edit source]

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