PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1428 [new locus tag: SAUSA300_RS07795 ]
• pan locus tag^?: SAUPAN001307000
• symbol: SAUSA300_1428
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1428 [new locus tag: SAUSA300_RS07795 ]
• symbol: SAUSA300_1428
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1586100..1586417
• length: 318
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915114 NCBI
• RefSeq: YP_494125 NCBI
• BioCyc: see SAUSA300_RS07795
• MicrobesOnline: 1292943 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGGCTGAAAATATTAAAACTGAACAACATTATTACACTAAAGATTTCTCAGGATACAGA
  AATGAAGAAGATAACTTTGTAGCAAATCAAGAATTGACAGTAACAATCACATTGAACGAG
  TACAGAAAACTTATTGAAATAAAGGCTGTTAAAGATAAAGAAGAAGATACTTACAGAGGT
  AAGTATTTTGCGGAAGAAAGAAAAAACGAAAAATTGGAAAAAGAAAATATAAAACTAAAA
  AACAAAATTTATGAATTACAAAACGAAGAAGATAACGAGGAGGACGAAGAAAACGAGGAG
  GAAGTCAACAATGACTAA
  60
  120
  180
  240
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  318
  

⊟Protein

⊟General

• locus tag: SAUSA300_1428 [new locus tag: SAUSA300_RS07795 ]
• symbol: SAUSA300_1428
• description: hypothetical protein
• length: 105
• theoretical pI: 4.20894
• theoretical MW: 12779.7
• GRAVY: -1.59238

⊟Function

• TIGRFAM:
• TheSEED  :
  • Phage protein
• PFAM:
  no clan defined Tox-REase-2; Restriction endonuclease fold toxin 2 (PF15646; HMM-score: 16.1)
  E-set (CL0159) Filamin; Filamin/ABP280 repeat (PF00630; HMM-score: 14.3)
  and 3 more
  no clan defined Rootletin; Ciliary rootlet component, centrosome cohesion (PF15035; HMM-score: 11)
  Tubulin (CL0566) Tubulin_2; Tubulin like (PF13809; HMM-score: 8.2)
  no clan defined Bap31; B-cell receptor-associated protein 31-like (PF05529; HMM-score: 7.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002124
  • TAT(Tat/SPI): 0.000212
  • LIPO(Sec/SPII): 0.000449
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160339 NCBI
• RefSeq: YP_494125 NCBI
• UniProt: A0A0H2XEB9 UniProt

⊟Protein sequence

• MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKLIEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEVNND

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications