PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1183 [new locus tag: SAUSA300_RS06395 ]
• pan locus tag^?: SAUPAN003593000
• symbol: SAUSA300_1183
• pan gene symbol^?: —
• synonym:
• product: 2-oxoglutarate ferredoxin oxidoreductase subunit beta

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1183 [new locus tag: SAUSA300_RS06395 ]
• symbol: SAUSA300_1183
• product: 2-oxoglutarate ferredoxin oxidoreductase subunit beta
• replicon: chromosome
• strand: +
• coordinates: 1302533..1303399
• length: 867
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913120 NCBI
• RefSeq: YP_493880 NCBI
• BioCyc: see SAUSA300_RS06395
• MicrobesOnline: 1292698 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGCGACATTTAAAGATTTTAGAAATAATGTTAAGCCTAACTGGTGCCCCGGATGTGGC
  GATTTCTCAGTACAAGCTGCAATTCAAAAAGCAGCCGCAAATATAGGGTTAGAACCTGAA
  GAAGTAGCTATCATCACCGGTATAGGATGTTCTGGCCGTCTTTCAGGATATATTAATTCT
  TATGGCGTTCATTCTATTCACGGACGTGCATTACCTTTAGCTCAAGGTGTAAAAATGGCG
  AATAAAGATTTAACTGTTATTGCATCGGGAGGAGATGGTGATGGTTATGCTATAGGTATG
  GGGCATACAATCCATGCTTTAAGAAGAAATATGAACATGACGTATATAGTCATGGATAAT
  CAAATTTATGGTTTGACAAAGGGACAAACATCGCCGTCATCAGCAGTAGGATTTGTTACT
  AAAACAACGCCAAAAGGTAATATAGAAAAAAATGTTGCGCCTTTAGAATTAGCATTATCA
  TCTGGTGCCACATTTGTAGCCCAAGGTTTTTCAAGCGATATTAAAGGATTAACAAAACTA
  ATTGAAGATGCAATTAATCATGATGGATTTTCATTCGTTAATGTCTTTTCACCATGTGTG
  ACTTATAATAAAATTAACACATACGATTGGTTTAAAGAACATTTAACAAGTGTTGATGAC
  ATTGAAAATTATGATTCTACAGATAAACAATTAGCGACTAAAACTGTTATTGAACATGAA
  TCTTTAGTAACTGGTATTGTTTATCAAGATAAAGAAACACCATCATATGAATCTCAAATT
  AAAGAGTTAGATGATACACCACTTGCTAAAAGAGATATCAAAATTACTGAAGACACCTTC
  AATGCATTAACTGAACAATTTATTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1183 [new locus tag: SAUSA300_RS06395 ]
• symbol: SAUSA300_1183
• description: 2-oxoglutarate ferredoxin oxidoreductase subunit beta
• length: 288
• theoretical pI: 5.14363
• theoretical MW: 31331.1
• GRAVY: -0.222222

⊟Function

• TIGRFAM:
  2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family (TIGR02177; HMM-score: 325.8)
  and 7 more
  indolepyruvate ferredoxin oxidoreductase, alpha subunit (TIGR03336; EC 1.2.7.8; HMM-score: 51.9)
  pyruvate:ferredoxin (flavodoxin) oxidoreductase (TIGR02176; EC 1.2.7.1; HMM-score: 43)
  sulfopyruvate decarboxylase, beta subunit (TIGR03846; EC 4.1.1.79; HMM-score: 25.4)
  Metabolism Energy metabolism Sugars 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase (TIGR04377; EC 3.7.1.-; HMM-score: 23.4)
  Metabolism Amino acid biosynthesis Pyruvate family acetolactate synthase, large subunit, biosynthetic type (TIGR00118; EC 2.2.1.6; HMM-score: 18)
  phosphonopyruvate decarboxylase (TIGR03297; EC 4.1.1.82; HMM-score: 17)
  Metabolism Central intermediary metabolism Other sulfoacetaldehyde acetyltransferase (TIGR03457; EC 2.3.3.15; HMM-score: 15.2)
• TheSEED  :
  • 2-oxoglutarate:ferredoxin oxidoreductase, beta subunit (EC 1.2.7.3)
  Carbohydrates Central carbohydrate metabolism TCA Cycle  2-oxoglutarate oxidoreductase, beta subunit (EC 1.2.7.3)
• PFAM:
  THDP-binding (CL0254) TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 112.4)
  and 1 more
  no clan defined PFO_beta_C; Pyruvate ferredoxin oxidoreductase beta subunit C terminal (PF12367; HMM-score: 77.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.035242
  • TAT(Tat/SPI): 0.002523
  • LIPO(Sec/SPII): 0.003897
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160150 NCBI
• RefSeq: YP_493880 NCBI
• UniProt: A0A0H2XFW0 UniProt

⊟Protein sequence

• MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAIITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGHTIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALSSGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSVDDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKITEDTFNALTEQFI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1182 > SAUSA300_1183

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications