PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1141 [new locus tag: SAUSA300_RS06175 ]
• pan locus tag^?: SAUPAN003542000
• symbol: SAUSA300_1141
• pan gene symbol^?: fmhC
• synonym:
• product: endopeptidase resistance protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1141 [new locus tag: SAUSA300_RS06175 ]
• symbol: SAUSA300_1141
• product: endopeptidase resistance protein
• replicon: chromosome
• strand: +
• coordinates: 1250301..1251545
• length: 1245
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912983 NCBI
• RefSeq: YP_493838 NCBI
• BioCyc: see SAUSA300_RS06175
• MicrobesOnline: 1292656 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAAATTTTCAACTTTAAGTGAAGAAGAATTTACCAACTACACCAAAAAGCACTTCAAA
  CATTATACACAGTCTATAGAATTATATAATTATAGAAATAAAATAAATCATGAAGCACAT
  ATTGTGGGAGTGAAGAATGATAAAAATGAAGTTATAGCTGCATGTTTATTAACAGAGGCA
  CGAATTTTTAAATTCTACAAATATTTCTACTCTCATAGAGGTCCTTTACTTGATTATTTC
  GATGCTAAATTAGTTTGTTACTTTTTTAAAGAATTATCTAAATTCATTTATAAAAATAGA
  GGAGTATTTATTCTTGTTGATCCATATTTAATAGAGAATTTAAGAGATGCAAATGGTAGG
  ATAATAAAGAATTATAATAATTCAGTGATAGTAAAGATGCTAGGGAAAATTGGGTATCTC
  CATCAAGGTTATACAACAGGATATTCAAATAAAAGTCAAATTAGGTGGATTTCTGTATTG
  GATTTAAAAGATAAAGATGAGAATCAACTTTTAAAAGAAATGGAATACCAAACTAGAAGA
  AATATAAAAAAGACTATTGAGATTGGTGTTAAGGTTGAAGATTTATCTATTGAAGAAACA
  AATCGATTTTATAAATTGTTTCAAATGGCTGAAGAAAAACATGGTTTTCATTTCATGAAT
  GAAGATTATTTTAAACGAATGCAAGAAATATATAAAGATAAGGCAATGTTAAAGATAGCT
  TGTATAAATCTTAATGAATATCAAGATAAATTAAAAATACAATTATTGAAAATCGAAAAT
  GAAATGATGACTGTGAACAGAGCATTAAATGAAAATCCAAATTCTAAAAAAAATAAATCA
  AAATTAAATCAGTTAAATATGCAATTATCTAGTATTAATAATAGAATTAGTAAAACCGAA
  GAACTAATATTTGAAGATGGACCTGTTTTGGATTTAGCTGCTGCTTTATTTATATGTACT
  GATGATGAAGTTTATTATCTATCAAGTGGATCAAATCCGAAATATAATCAGTATATGGGT
  GCATATCATCTACAATGGCATATGATAAAATATGCAAAATCACATAATATTAATAGGTAT
  AATTTTTATGGAATAACAGGCGTCTTTAGTAATGAGGCGGATGATTTTGGTGTTCAACAA
  TTTAAAAAGGGTTTTAATGCACATGTTGAAGAATTAATTGGTGATTTCATCAAACCAGTA
  AGACCAATTCTATATAAATTTGCAAAACTTATTTATAAGGTTTAA
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  1245
  

⊟Protein

⊟General

• locus tag: SAUSA300_1141 [new locus tag: SAUSA300_RS06175 ]
• symbol: SAUSA300_1141
• description: endopeptidase resistance protein
• length: 414
• theoretical pI: 9.38877
• theoretical MW: 49116.2
• GRAVY: -0.519807

⊟Function

• TIGRFAM:
  Unknown function General FemAB-related protein, PEP-CTERM system-associated (TIGR03019; HMM-score: 22.4)
• TheSEED  :
  • FmhC protein of FemAB family
• PFAM:
  Acetyltrans (CL0257) FemAB; FemAB family (PF02388; HMM-score: 518.7)
  and 6 more
  Acetyltransf_6; Acetyltransferase (GNAT) domain (PF13480; HMM-score: 28.2)
  no clan defined HIND; HIND motif (PF19252; HMM-score: 13.7)
  Snapin_Pallidin; Snapin/Pallidin (PF14712; HMM-score: 11.4)
  EAD9; Effector-associated domain 9 (PF19962; HMM-score: 9.8)
  Acetyltrans (CL0257) FemAB_like; Peptidogalycan biosysnthesis/recognition (PF04339; HMM-score: 8.5)
  no clan defined GED; Dynamin GTPase effector domain (PF02212; HMM-score: 7.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.4119
  • Cytoplasmic Membrane Score: 0.443
  • Cell wall & surface Score: 0.0016
  • Extracellular Score: 0.1436
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.013322
  • TAT(Tat/SPI): 0.000284
  • LIPO(Sec/SPII): 0.002296
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160130 NCBI
• RefSeq: YP_493838 NCBI
• UniProt: A0A0H2XEE6 UniProt

⊟Protein sequence

• MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGVKNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGVFILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVLDLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHFMNEDYFKRMQEIYKDKAMLKIACINLNEYQDKLKIQLLKIENEMMTVNRALNENPNSKKNKSKLNQLNMQLSSINNRISKTEELIFEDGPVLDLAAALFICTDDEVYYLSSGSNPKYNQYMGAYHLQWHMIKYAKSHNINRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEELIGDFIKPVRPILYKFAKLIYKV

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: lytN > SAUSA300_1141

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

FmhC binds to FemB to alter assembly of the pentaglycine cross-bridge linking staphylococcal peptidoglycan. This change converts the fifth glycine of the pentaglycine cross-bridge into a serine providing resistance to lysostaphin and similar staphylococcal cell wall hydrolases. Incorporation of serine at position 5, however, does decrease resistance to beta lactam antibiotics.

⊟Literature

⊟References

⊟Relevant publications