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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1122 [new locus tag: SAUSA300_RS06070 ]
  • pan locus tag?: SAUPAN003513000
  • symbol: plsX
  • pan gene symbol?: plsX
  • synonym:
  • product: putative glycerol-3-phosphate acyltransferase PlsX

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1122 [new locus tag: SAUSA300_RS06070 ]
  • symbol: plsX
  • product: putative glycerol-3-phosphate acyltransferase PlsX
  • replicon: chromosome
  • strand: +
  • coordinates: 1228616..1229602
  • length: 987
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGTTAAATTAGCAATTGATATGATGGGTGGCGACAATGCGCCTGATATCGTATTAGAA
    GCCGTACAAAAGGCTGTTGAAGACTTTAAAGATCTAGAAATTATACTTTTCGGTGACGAA
    AAAAAGTATAATCTGAACCATGAACGAATCGAATTTAGACATTGTTCTGAAAAGATTGAA
    ATGGAAGATGAGCCTGTTAGAGCGATTAAACGTAAAAAAGATAGCTCAATGGTAAAAATG
    GCTGAAGCTGTGAAATCTGGTGAAGCAGATGGATGTGTGTCAGCAGGTAATACTGGTGCT
    TTAATGTCAGCTGGTTTATTCATTGTTGGACGTATTAAAGGTGTAGCTAGACCGGCTTTA
    GTAGTAACATTGCCAACGATTGATGGAAAAGGTTTTGTCTTTTTAGACGTTGGTGCAAAT
    GCTGATGCTAAACCTGAACACTTATTACAGTATGCGCAACTAGGGGATATTTATGCTCAA
    AAAATTAGAGGTATTGATAATCCGAAAATCTCATTATTAAATATAGGAACCGAGCCAGCT
    AAAGGTAATAGTTTAACGAAAAAATCATATGAGTTATTAAATCATGATCATTCATTGAAT
    TTTGTTGGGAATATTGAAGCGAAGACATTAATGGATGGCGATACAGATGTTGTAGTTACC
    GATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGT
    AAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTG
    AAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTA
    TTATTAGGATTGGAAGGTACTGTAGTTAAAGCACACGGTAGTTCAAATGCTAAAGCTTTT
    TATTCTGCAATTAGACAAGCGAAAATCGCAGGAGAACAAAATATTGTACAAACAATGAAA
    GAGACTGTAGGTGAATCAAATGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    987

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1122 [new locus tag: SAUSA300_RS06070 ]
  • symbol: PlsX
  • description: putative glycerol-3-phosphate acyltransferase PlsX
  • length: 328
  • theoretical pI: 6.27376
  • theoretical MW: 35430.5
  • GRAVY: -0.218902

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.n2?  ExPASy
    Phosphate acyltransferase Acyl-[acyl-carrier-protein] + phosphate = acyl-phosphate + [acyl-carrier-protein]
  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis fatty acid/phospholipid synthesis protein PlsX (TIGR00182; HMM-score: 408.3)
  • TheSEED  :
    • Phosphate:acyl-ACP acyltransferase PlsX
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Phosphate:acyl-ACP acyltransferase PlsX
  • PFAM:
    Iso_DH (CL0270) FA_synthesis; Fatty acid synthesis protein (PF02504; HMM-score: 369.8)
    and 1 more
    PTA_PTB; Phosphate acetyl/butaryl transferase (PF01515; HMM-score: 18)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001652
    • TAT(Tat/SPI): 0.000124
    • LIPO(Sec/SPII): 0.000518
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYNLNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALMSAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQKIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVVVTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEYGGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: FapR* (repression) regulon
    FapR*(TF)important in Fatty acid biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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